The University of Southampton
University of Southampton Institutional Repository

Influence of temperature and plumbing material selection on biofilm formation and growth of Legionella pneumophila in a model potable water system containing complex microbial flora

Influence of temperature and plumbing material selection on biofilm formation and growth of Legionella pneumophila in a model potable water system containing complex microbial flora
Influence of temperature and plumbing material selection on biofilm formation and growth of Legionella pneumophila in a model potable water system containing complex microbial flora

Survival and growth of Legionella pneumophila in both biofilm and planktonic phases were determined with a two-stage model system. The model used filter-sterilized tap water as the sole source of nutrient to culture a naturally occurring mixed population of microorganisms including virulent L. pneumophila. At 20°C, L. pneumophila accounted for a low proportion of biofilm flora on polybutylene and chlorinated polyvinyl chloride, but was absent from copper surfaces. The pathogen was most abundant on biofilms on plastics at 40°C, where if accounted for up to 50% of the total biofilm flora. Copper surfaces were inhibitory to total biofouling and included only low numbers of L. pneumophila organisms. The pathogen was able to survive in biofilms on the surface of the plastic materials at 50°C, but was absent from the copper surfaces at the same temperature. L. pneumophila could not be detected in the model system at 60°C. In the presence of copper surfaces, biofilms forming on adjacent control glass surfaces were found to incorporate copper ions which subsequently inhibited colonization of their surfaces. This work suggests that the use of copper tubing in water systems may help to limit the colonization of water systems by L. pneumophila.

0099-2240
1585-1592
Rogers, J.
2c2865dd-0bad-4130-b4bf-f9d77c8158c9
Dowsett, A. B.
fb65d746-cf78-4004-8773-9858a4e4f01c
Dennis, P. J.
25bd2523-2802-498f-9a17-80ecea7db112
Lee, J. V.
07a58a30-a604-4d98-ae61-6edbd92aaae2
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Rogers, J.
2c2865dd-0bad-4130-b4bf-f9d77c8158c9
Dowsett, A. B.
fb65d746-cf78-4004-8773-9858a4e4f01c
Dennis, P. J.
25bd2523-2802-498f-9a17-80ecea7db112
Lee, J. V.
07a58a30-a604-4d98-ae61-6edbd92aaae2
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

Rogers, J., Dowsett, A. B., Dennis, P. J., Lee, J. V. and Keevil, C. W. (1994) Influence of temperature and plumbing material selection on biofilm formation and growth of Legionella pneumophila in a model potable water system containing complex microbial flora. Applied and Environmental Microbiology, 60 (5), 1585-1592.

Record type: Article

Abstract

Survival and growth of Legionella pneumophila in both biofilm and planktonic phases were determined with a two-stage model system. The model used filter-sterilized tap water as the sole source of nutrient to culture a naturally occurring mixed population of microorganisms including virulent L. pneumophila. At 20°C, L. pneumophila accounted for a low proportion of biofilm flora on polybutylene and chlorinated polyvinyl chloride, but was absent from copper surfaces. The pathogen was most abundant on biofilms on plastics at 40°C, where if accounted for up to 50% of the total biofilm flora. Copper surfaces were inhibitory to total biofouling and included only low numbers of L. pneumophila organisms. The pathogen was able to survive in biofilms on the surface of the plastic materials at 50°C, but was absent from the copper surfaces at the same temperature. L. pneumophila could not be detected in the model system at 60°C. In the presence of copper surfaces, biofilms forming on adjacent control glass surfaces were found to incorporate copper ions which subsequently inhibited colonization of their surfaces. This work suggests that the use of copper tubing in water systems may help to limit the colonization of water systems by L. pneumophila.

Full text not available from this repository.

More information

Published date: 1 May 1994

Identifiers

Local EPrints ID: 431336
URI: https://eprints.soton.ac.uk/id/eprint/431336
ISSN: 0099-2240
PURE UUID: 087d8758-1768-4ef5-805d-0326d08404ce
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 29 May 2019 16:30
Last modified: 30 May 2019 00:36

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×