The University of Southampton
University of Southampton Institutional Repository

Comparison of selective agars for the isolation and identification of Klebsiella oxytoca and Escherichia coli from environmental drinking water samples

Comparison of selective agars for the isolation and identification of Klebsiella oxytoca and Escherichia coli from environmental drinking water samples
Comparison of selective agars for the isolation and identification of Klebsiella oxytoca and Escherichia coli from environmental drinking water samples

Various selective media were assessed for their ability to detect and differentiate Klebsiella oxytoca and Escherichia coli in environmental water samples. Only two, Membrane Lauryl Sulphate agar and Deoxycholate Agar, could differentiate the two coliforms from each other and from the ‘background’ heterotrophs in water and this was a consequence of E. coli's ability to grow at 44°C and 37°C whereas Kl. oxytoca could only grow at 37°C. Modified M‐FC medium effectively differentiated Kl. oxytoca but not E. coli in environmental samples. Other media characterized the different coliforms in pure culture but failed to do likewise in environmental samples. For example, pure cultures of E. coli fluoresced when MUG was added to the medium but single colonies on a mixed species plate failed to do so. MT7 agar distinguished the two coliforms from water heterotrophs but not from each other.

0266-8254
303-307
Packer, P. J.
eae01aba-76b6-4784-9dab-d4e9d3921fe6
Mackerness, C. W.
414804a3-28d0-4b56-99c0-ac77c1f37ca3
Riches, M.
2b8c45c6-b310-40fb-83c0-e26d2473ab10
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Packer, P. J.
eae01aba-76b6-4784-9dab-d4e9d3921fe6
Mackerness, C. W.
414804a3-28d0-4b56-99c0-ac77c1f37ca3
Riches, M.
2b8c45c6-b310-40fb-83c0-e26d2473ab10
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

Packer, P. J., Mackerness, C. W., Riches, M. and Keevil, C. W. (1995) Comparison of selective agars for the isolation and identification of Klebsiella oxytoca and Escherichia coli from environmental drinking water samples. Letters in Applied Microbiology, 20 (5), 303-307. (doi:10.1111/j.1472-765X.1995.tb00451.x).

Record type: Article

Abstract

Various selective media were assessed for their ability to detect and differentiate Klebsiella oxytoca and Escherichia coli in environmental water samples. Only two, Membrane Lauryl Sulphate agar and Deoxycholate Agar, could differentiate the two coliforms from each other and from the ‘background’ heterotrophs in water and this was a consequence of E. coli's ability to grow at 44°C and 37°C whereas Kl. oxytoca could only grow at 37°C. Modified M‐FC medium effectively differentiated Kl. oxytoca but not E. coli in environmental samples. Other media characterized the different coliforms in pure culture but failed to do likewise in environmental samples. For example, pure cultures of E. coli fluoresced when MUG was added to the medium but single colonies on a mixed species plate failed to do so. MT7 agar distinguished the two coliforms from water heterotrophs but not from each other.

Full text not available from this repository.

More information

Accepted/In Press date: 20 December 1994
Published date: May 1995

Identifiers

Local EPrints ID: 431343
URI: http://eprints.soton.ac.uk/id/eprint/431343
ISSN: 0266-8254
PURE UUID: fe3749cc-18a0-4ca2-8d67-805f88a1b581
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 29 May 2019 16:30
Last modified: 30 May 2019 00:36

Export record

Altmetrics

Contributors

Author: P. J. Packer
Author: C. W. Mackerness
Author: M. Riches
Author: C. W. Keevil ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×