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Coaggregation amongst aquatic biofilm bacteria

Coaggregation amongst aquatic biofilm bacteria
Coaggregation amongst aquatic biofilm bacteria

Aquatic autochthonous flora isolated directly from a water biofilm model was tested for its capacity to take part in coaggregation. Two assay methods were compared and a visual semi-quantitative method was found to be the most consistent and easy to use. All isolates demonstrated coaggregation with at least one other isolate to some extent, giving rise to visible flocs. One isolate, identified as Micrococcus luteus, was found to coaggregate with all of the other isolates to different degrees, highlighting a possible role as a bridging organism in the biofilm consortium. The addition of certain simple sugars reversed coaggregation between particular isolates suggesting that lectin-like adhesins were operating between these members of the aquatic autochthonous flora by a mechanism similar to that reported to occur between dental plaque bacteria. The time taken for coaggregation to begin varied considerably between different pairs of isolates (1-16 h), suggesting that this may play a role in determining the successional order of isolates during biofilm development.

1364-5072
477-484
Buswell, C. M.
0d632734-a8a8-4f9d-8eef-6c25b00fe725
Herlihy, Y. M.
4522a096-5dce-40ae-ad9b-a321ac6aced2
Marsh, P. D.
9d226405-bfd2-432b-ac22-ea619f706805
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Leach, S. A.
33d08c43-c8b8-4f9b-9232-ad887ac6026d
Buswell, C. M.
0d632734-a8a8-4f9d-8eef-6c25b00fe725
Herlihy, Y. M.
4522a096-5dce-40ae-ad9b-a321ac6aced2
Marsh, P. D.
9d226405-bfd2-432b-ac22-ea619f706805
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Leach, S. A.
33d08c43-c8b8-4f9b-9232-ad887ac6026d

Buswell, C. M., Herlihy, Y. M., Marsh, P. D., Keevil, C. W. and Leach, S. A. (1997) Coaggregation amongst aquatic biofilm bacteria. Journal of Applied Microbiology, 83 (4), 477-484. (doi:10.1046/j.1365-2672.1997.00260.x).

Record type: Article

Abstract

Aquatic autochthonous flora isolated directly from a water biofilm model was tested for its capacity to take part in coaggregation. Two assay methods were compared and a visual semi-quantitative method was found to be the most consistent and easy to use. All isolates demonstrated coaggregation with at least one other isolate to some extent, giving rise to visible flocs. One isolate, identified as Micrococcus luteus, was found to coaggregate with all of the other isolates to different degrees, highlighting a possible role as a bridging organism in the biofilm consortium. The addition of certain simple sugars reversed coaggregation between particular isolates suggesting that lectin-like adhesins were operating between these members of the aquatic autochthonous flora by a mechanism similar to that reported to occur between dental plaque bacteria. The time taken for coaggregation to begin varied considerably between different pairs of isolates (1-16 h), suggesting that this may play a role in determining the successional order of isolates during biofilm development.

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More information

Published date: September 1997

Identifiers

Local EPrints ID: 431347
URI: http://eprints.soton.ac.uk/id/eprint/431347
ISSN: 1364-5072
PURE UUID: bd9db260-2756-4247-8c94-34132cbbf85f
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

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Date deposited: 29 May 2019 16:30
Last modified: 16 Mar 2024 03:24

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Contributors

Author: C. M. Buswell
Author: Y. M. Herlihy
Author: P. D. Marsh
Author: C. W. Keevil ORCID iD
Author: S. A. Leach

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