A simple artificial urine for the growth of urinary pathogens
A simple artificial urine for the growth of urinary pathogens
A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10
8
cfu ml
-1
, as found in normal urine. Reproducible, steady-state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.
203-206
Brooks, T.
5874d729-1ec3-4045-88ae-c271be824867
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
March 1997
Brooks, T.
5874d729-1ec3-4045-88ae-c271be824867
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Brooks, T. and Keevil, C. W.
(1997)
A simple artificial urine for the growth of urinary pathogens.
Letters in Applied Microbiology, 24 (3), .
(doi:10.1046/j.1472-765X.1997.00378.x).
Abstract
A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10
8
cfu ml
-1
, as found in normal urine. Reproducible, steady-state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.
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Published date: March 1997
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Local EPrints ID: 431348
URI: http://eprints.soton.ac.uk/id/eprint/431348
ISSN: 0266-8254
PURE UUID: 93220330-0328-4003-967e-75578aa89c35
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Date deposited: 29 May 2019 16:30
Last modified: 16 Mar 2024 03:24
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Author:
T. Brooks
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