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A study of iron acquisition mechanisms of Legionella pneumophila grown in chemostat culture

A study of iron acquisition mechanisms of Legionella pneumophila grown in chemostat culture
A study of iron acquisition mechanisms of Legionella pneumophila grown in chemostat culture

We recently demonstrated that the virulence of a clinical isolate of Legionella pneumophila is significantly attenuated when cultured in an iron- limited environment. In this study the influence of iron limitation on the expression of enzyme activities and iron-transport mechanisms was investigated. Expression of the important pathogenicity factor, the zinc metalloprotease, was reduced fivefold in response to iron limitation. Ferric citrate reductase activity was demonstrated in both iron-limited and replete cell fractions. Activity was located principally in the cytoplasm and periplasm, and was not enhanced by iron restriction. Optimum activity was observed with NADPH as reductant. Siderophores were not elaborated under these culture conditions. Iron-loaded transferrin enhanced the growth of steady-state, iron-limited cultures, demonstrating that transferrin represents a potentially important iron source for L. pneumophila in vivo. Although cell surface transferrin receptors were not detected, in vitro experiments demonstrated digestion of transferrin by the zinc metalloprotease activity of culture supernatants.

0343-8651
238-243
James, Brian W.
2982ef34-33f6-4dd1-bc54-03b0333f3339
Mauchline, W. Stuart
80ed42b2-235d-4e4b-998b-bb928a52daa2
Dennis, P. Julian
25bd2523-2802-498f-9a17-80ecea7db112
Keevil, C. William
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
James, Brian W.
2982ef34-33f6-4dd1-bc54-03b0333f3339
Mauchline, W. Stuart
80ed42b2-235d-4e4b-998b-bb928a52daa2
Dennis, P. Julian
25bd2523-2802-498f-9a17-80ecea7db112
Keevil, C. William
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

James, Brian W., Mauchline, W. Stuart, Dennis, P. Julian and Keevil, C. William (1997) A study of iron acquisition mechanisms of Legionella pneumophila grown in chemostat culture. Current Microbiology, 34 (4), 238-243. (doi:10.1007/s002849900176).

Record type: Article

Abstract

We recently demonstrated that the virulence of a clinical isolate of Legionella pneumophila is significantly attenuated when cultured in an iron- limited environment. In this study the influence of iron limitation on the expression of enzyme activities and iron-transport mechanisms was investigated. Expression of the important pathogenicity factor, the zinc metalloprotease, was reduced fivefold in response to iron limitation. Ferric citrate reductase activity was demonstrated in both iron-limited and replete cell fractions. Activity was located principally in the cytoplasm and periplasm, and was not enhanced by iron restriction. Optimum activity was observed with NADPH as reductant. Siderophores were not elaborated under these culture conditions. Iron-loaded transferrin enhanced the growth of steady-state, iron-limited cultures, demonstrating that transferrin represents a potentially important iron source for L. pneumophila in vivo. Although cell surface transferrin receptors were not detected, in vitro experiments demonstrated digestion of transferrin by the zinc metalloprotease activity of culture supernatants.

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More information

Published date: 1 April 1997

Identifiers

Local EPrints ID: 431349
URI: http://eprints.soton.ac.uk/id/eprint/431349
ISSN: 0343-8651
PURE UUID: 4ff82f6b-937d-48af-b87e-76b09e56f80d
ORCID for C. William Keevil: ORCID iD orcid.org/0000-0003-1917-7706

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Date deposited: 29 May 2019 16:30
Last modified: 10 Nov 2021 03:04

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Contributors

Author: Brian W. James
Author: W. Stuart Mauchline
Author: P. Julian Dennis

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