Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics
Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics
Atomic-level information about the structure and dynamics of biomolecules is critical for an understanding of their function. Nuclear magnetic resonance (NMR) spectroscopy provides unique insights into the dynamic nature of biomolecules and their interactions, capturing transient conformers and their features. However, relaxation-induced line broadening and signal overlap make it challenging to apply NMR spectroscopy to large biological systems. Here we took advantage of the high sensitivity and broad chemical shift range of 19F nuclei and leveraged the remarkable relaxation properties of the aromatic 19F-13C spin pair to disperse 19F resonances in a two-dimensional transverse relaxation-optimized spectroscopy spectrum. We demonstrate the application of 19F-13C transverse relaxation-optimized spectroscopy to investigate proteins and nucleic acids. This experiment expands the scope of 19F NMR in the study of the structure, dynamics, and function of large and complex biological systems and provides a powerful background-free NMR probe.
333-340
Boeszoermenyi, Andras
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Chhabra, Sandeep
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Dubey, Abhinav
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Radeva, Denitsa L.
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Burdzhiev, Nikola T.
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Chanev, Christo D.
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Petrov, Ognyan I.
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Gelev, Vladimir M.
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Zhang, Meng
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Anklin, Clemens
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Kovacs, Helena
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Wagner, Gerhard
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Kuprov, Ilya
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Takeuchi, Koh
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Arthanari, Haribabu
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Boeszoermenyi, Andras
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Chhabra, Sandeep
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Dubey, Abhinav
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Radeva, Denitsa L.
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Burdzhiev, Nikola T.
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Chanev, Christo D.
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Petrov, Ognyan I.
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Gelev, Vladimir M.
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Zhang, Meng
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Anklin, Clemens
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Kovacs, Helena
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Wagner, Gerhard
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Kuprov, Ilya
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Takeuchi, Koh
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Arthanari, Haribabu
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Boeszoermenyi, Andras, Chhabra, Sandeep, Dubey, Abhinav, Radeva, Denitsa L., Burdzhiev, Nikola T., Chanev, Christo D., Petrov, Ognyan I., Gelev, Vladimir M., Zhang, Meng, Anklin, Clemens, Kovacs, Helena, Wagner, Gerhard, Kuprov, Ilya, Takeuchi, Koh and Arthanari, Haribabu
(2019)
Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics.
Nature Methods, 16, .
(doi:10.1038/s41592-019-0334-x).
Abstract
Atomic-level information about the structure and dynamics of biomolecules is critical for an understanding of their function. Nuclear magnetic resonance (NMR) spectroscopy provides unique insights into the dynamic nature of biomolecules and their interactions, capturing transient conformers and their features. However, relaxation-induced line broadening and signal overlap make it challenging to apply NMR spectroscopy to large biological systems. Here we took advantage of the high sensitivity and broad chemical shift range of 19F nuclei and leveraged the remarkable relaxation properties of the aromatic 19F-13C spin pair to disperse 19F resonances in a two-dimensional transverse relaxation-optimized spectroscopy spectrum. We demonstrate the application of 19F-13C transverse relaxation-optimized spectroscopy to investigate proteins and nucleic acids. This experiment expands the scope of 19F NMR in the study of the structure, dynamics, and function of large and complex biological systems and provides a powerful background-free NMR probe.
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NIHMS1520040
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Boeszoermenyi_2019_Aromatic_19F-13C_TROSY_a_background-free_approach_to_probe_biomolecular_structrue_function_and_dynamics
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Accepted/In Press date: 30 January 2019
e-pub ahead of print date: 11 March 2019
Identifiers
Local EPrints ID: 431493
URI: http://eprints.soton.ac.uk/id/eprint/431493
ISSN: 1548-7091
PURE UUID: 2557753f-2796-4bd2-9522-92abc30ce2b3
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Date deposited: 05 Jun 2019 16:30
Last modified: 16 Mar 2024 07:41
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Contributors
Author:
Andras Boeszoermenyi
Author:
Sandeep Chhabra
Author:
Abhinav Dubey
Author:
Denitsa L. Radeva
Author:
Nikola T. Burdzhiev
Author:
Christo D. Chanev
Author:
Ognyan I. Petrov
Author:
Vladimir M. Gelev
Author:
Meng Zhang
Author:
Clemens Anklin
Author:
Helena Kovacs
Author:
Gerhard Wagner
Author:
Koh Takeuchi
Author:
Haribabu Arthanari
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