READ ME File For 'Data for Mast cell mediators: their sub cellular localisation, bacterial influence on their release and expression and their potential value as markers for predicting the severity of allergic reactions'

Dataset DOI: 10.5258/SOTON/D0947

ReadMe Author: Rana S. Abadalkareem, University of Southampton

This dataset supports the thesis entitled "Mast cell mediators: their sub cellular localisation, bacterial influence on their release and expression and their potential value as markers for predicting the severity of allergic reactions"
AWARDED BY: Faculty of Medicine, University of Southampton
DATE OF AWARD: 1st May 2018

DESCRIPTION OF THE DATA
This PhD project focused on the role of mast cell proteases in predicting the severity of allergic reactions to drugs or food. The project involved analysis of serum levels of mast cell proteases and a panel of pro-inflammatory cytokines in patients with allergic reactions provoked by drugs or food. The clinical and immunological data were combined and analysed by topological data analysis. In addition, factors that can modulate the release and expression of mast cell mediators and their subcellular localization were investigated. 
We found that carboxypeptidase A3 (CPA3), a mast cell protease, have the potential to predict the severity of allergic reactions. Topological data analysis identified four novel multidimensional endotypes. Studies with LAD2 mast cell line culture showed that S. aureus could have an inhibitory effect on release and expression of mast cell mediators.  
The potential for bacterial infection to interfere with mast cell responses could reduce the susceptibility to allergic reactions and as the mechanisms involved deserve consideration as a novel therapeutic approach to prevent development of severe reactions. Serum levels of CPA3 have the potential to predict the severity of allergic reactions to drugs or food. Identification of four multidimensional endotypes underlines the connection between levels of mast cell proteases and pro-inflammatory cytokines and their association with clinical features of patients who have drug or food allergies. The application of the characteristics of these endotypes clinically can help to identify those at particular risk of allergic reactions and allow optimal interventions to be undertaken


This dataset contains:
Spreadsheets and SPSS files of clinical and immunological parameters (log transformed) for two cohort studies (Southampton and Qatar). It also contains prism files of the effect of Staph aureus infection on the sensitization of LAD2 mast cell line, including levels of b-hexasominidase and cytokines and gene expression results. The results of MAPK analysis is also included (spreadsheet and prism file)




Date of data collection: Clinical data was collected from January 2015 to December 2016

Information about geographic location of data collection: University of Southampton, U.K.

Ethical approval: The study was approved by the Isle of Wight, Portsmouth and South East Hampshire Region Ethics Committee (registration number 08-H0501-17), and by the University Hospital Southampton NHS Foundation Trust Research and Development Department

Related projects/Funders:
The project was entirely funded by Iraqi Ministry of Higher Education and Scientific Research


Date that the file was created: May, 2019