Memristive biosensors based on full-size antibodies and antibody fragments
Memristive biosensors based on full-size antibodies and antibody fragments
For the first time memristive biosensors developed by the incorporation of scAbs (single-chain antibody fragments) enabling label-free detection of free Prostate Specific Antigen (fPSA) for early-stage detection of prostate cancer (PC) are reported. In addition, the first ever-published report of a direct relationship between the size of the bio-probes used to transfer specificity to the sensor and the voltage gap is here included. The proposed bio-detection is achieved by means of a voltage gap acquired in the electrical response of memristive sensors obtained by the bio-modification of silicon nanowire arrays. A comparison between memristive biosensors based on full-size antibodies and antibody fragments is also demonstrated by analysis of the surface morphology via Scanning Electron Microscopy. This new kind of biosensors provide excellent analytical sensitivity with a limit-of-detection down to 0.144 pg/mL on PSA.
Antibody fragments, Cancer diagnosis, Memristive biosensors, scAb, Silicon nanowires
346-352
Tzouvadaki, Ioulia
a1025ec1-7606-453d-bc71-1f732a4c1f78
Zapatero-Rodríguez, Julia
dd6b1587-412d-45b5-a61f-8faa78129e7c
Naus, Sébastien
c2259f76-28f6-4169-aba9-6b00788bedf6
de Micheli, Giovanni
23af8e38-a795-4edf-b551-9094fdb781e0
O'Kennedy, Richard
d1196faa-86b1-4529-9a7f-5e39b09fd3f5
Carrara, Sandro
0001b4c5-1f62-4789-b0e9-5a187f58b893
1 May 2019
Tzouvadaki, Ioulia
a1025ec1-7606-453d-bc71-1f732a4c1f78
Zapatero-Rodríguez, Julia
dd6b1587-412d-45b5-a61f-8faa78129e7c
Naus, Sébastien
c2259f76-28f6-4169-aba9-6b00788bedf6
de Micheli, Giovanni
23af8e38-a795-4edf-b551-9094fdb781e0
O'Kennedy, Richard
d1196faa-86b1-4529-9a7f-5e39b09fd3f5
Carrara, Sandro
0001b4c5-1f62-4789-b0e9-5a187f58b893
Tzouvadaki, Ioulia, Zapatero-Rodríguez, Julia, Naus, Sébastien, de Micheli, Giovanni, O'Kennedy, Richard and Carrara, Sandro
(2019)
Memristive biosensors based on full-size antibodies and antibody fragments.
Sensors and Actuators, B: Chemical, 286, .
(doi:10.1016/j.snb.2019.02.001).
Abstract
For the first time memristive biosensors developed by the incorporation of scAbs (single-chain antibody fragments) enabling label-free detection of free Prostate Specific Antigen (fPSA) for early-stage detection of prostate cancer (PC) are reported. In addition, the first ever-published report of a direct relationship between the size of the bio-probes used to transfer specificity to the sensor and the voltage gap is here included. The proposed bio-detection is achieved by means of a voltage gap acquired in the electrical response of memristive sensors obtained by the bio-modification of silicon nanowire arrays. A comparison between memristive biosensors based on full-size antibodies and antibody fragments is also demonstrated by analysis of the surface morphology via Scanning Electron Microscopy. This new kind of biosensors provide excellent analytical sensitivity with a limit-of-detection down to 0.144 pg/mL on PSA.
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More information
Accepted/In Press date: 1 February 2019
e-pub ahead of print date: 1 February 2019
Published date: 1 May 2019
Keywords:
Antibody fragments, Cancer diagnosis, Memristive biosensors, scAb, Silicon nanowires
Identifiers
Local EPrints ID: 431543
URI: http://eprints.soton.ac.uk/id/eprint/431543
ISSN: 0925-4005
PURE UUID: 7e8b5cbf-e38c-4f07-a45e-af9cb89698ca
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Date deposited: 07 Jun 2019 16:30
Last modified: 17 Mar 2024 12:27
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Contributors
Author:
Ioulia Tzouvadaki
Author:
Julia Zapatero-Rodríguez
Author:
Sébastien Naus
Author:
Giovanni de Micheli
Author:
Richard O'Kennedy
Author:
Sandro Carrara
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