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Microstructured hybrid scaffolds for aligning neonatal rat ventricular myocytes

Microstructured hybrid scaffolds for aligning neonatal rat ventricular myocytes
Microstructured hybrid scaffolds for aligning neonatal rat ventricular myocytes
In cardiac tissue engineering (TE), in vitro models are essential for the study of healthy and pathological heart tissues in order to understand the underpinning mechanisms. In this scenario, scaffolds are platforms that can realistically mimic the natural architecture of the heart, and they add biorealism to in vitro models. This paper reports a novel and robust technique to fabricate cardiovascular-mimetic scaffolds based on Parylene C and Polydimethylsiloxane (PDMS). Parylene C is employed as a mask material for inducing hybrid and non-hybrid micropatterns to the PDMS layer. Hybrid architectures present striped hydrophobic/hydrophilic surfaces, whereas non-hybrid scaffolds only corrugated topographies. Herein, we demonstrate that wavy features on PDMS can be obtained at the micro- and nanoscale and that PDMS can be integrated into the microfabrication process without changing its intrinsic physical properties. A study of the effects of these scaffolds on the growth of Neonatal Rat Ventricular Myocytes (NRVMs) cultures reveals that cell alignment occurs only for the case of hybrid architectures made of hydrophilic PDMS and hydrophobic Parylene C.
0928-4931
1-11
Sanzari, Ilaria
0044ac56-579f-4666-8ae1-47839b9742bf
Dinelli, F.
0316eeb9-41bd-4feb-abc1-aac06704f46e
Humphrey, Eleanor
87358a32-d973-46c2-af9f-a7cbd0684c42
Prodromakis, Themis
d58c9c10-9d25-4d22-b155-06c8437acfbf
Sanzari, Ilaria
0044ac56-579f-4666-8ae1-47839b9742bf
Dinelli, F.
0316eeb9-41bd-4feb-abc1-aac06704f46e
Humphrey, Eleanor
87358a32-d973-46c2-af9f-a7cbd0684c42
Prodromakis, Themis
d58c9c10-9d25-4d22-b155-06c8437acfbf

Sanzari, Ilaria, Dinelli, F., Humphrey, Eleanor and Prodromakis, Themis (2019) Microstructured hybrid scaffolds for aligning neonatal rat ventricular myocytes. Materials Science and Engineering C, 103, 1-11. (doi:10.1016/j.msec.2019.109783).

Record type: Article

Abstract

In cardiac tissue engineering (TE), in vitro models are essential for the study of healthy and pathological heart tissues in order to understand the underpinning mechanisms. In this scenario, scaffolds are platforms that can realistically mimic the natural architecture of the heart, and they add biorealism to in vitro models. This paper reports a novel and robust technique to fabricate cardiovascular-mimetic scaffolds based on Parylene C and Polydimethylsiloxane (PDMS). Parylene C is employed as a mask material for inducing hybrid and non-hybrid micropatterns to the PDMS layer. Hybrid architectures present striped hydrophobic/hydrophilic surfaces, whereas non-hybrid scaffolds only corrugated topographies. Herein, we demonstrate that wavy features on PDMS can be obtained at the micro- and nanoscale and that PDMS can be integrated into the microfabrication process without changing its intrinsic physical properties. A study of the effects of these scaffolds on the growth of Neonatal Rat Ventricular Myocytes (NRVMs) cultures reveals that cell alignment occurs only for the case of hybrid architectures made of hydrophilic PDMS and hydrophobic Parylene C.

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More information

Submitted date: 12 November 2018
Accepted/In Press date: 20 May 2019
e-pub ahead of print date: 21 May 2019

Identifiers

Local EPrints ID: 431774
URI: https://eprints.soton.ac.uk/id/eprint/431774
ISSN: 0928-4931
PURE UUID: ec44ef16-666a-46cd-908b-830d276b1ae9
ORCID for Themis Prodromakis: ORCID iD orcid.org/0000-0002-6267-6909

Catalogue record

Date deposited: 14 Jun 2019 16:30
Last modified: 20 Jul 2019 00:38

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