Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae
Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae
Neisseria gonorrhoeae is a gram-negative obligate human pathogen that contains lipooligosaccharide (LOS) as a major constituent within the outer membrane. LOS plays a major role in pathogenesis by inducing host inflammatory responses and also enabling evasion of host innate immunity through sialylation. Epitopes within LOS are also potential vaccine candidates. In this chapter, we describe a general method based on the Westphal hot phenol extraction process to purify whole LOS from N. gonorrhoeae for structural analyses and for use in in vivo and in vitro biological assays.
Electrodialysis, Lipooligosaccharide, LOS, Phenol extraction, Silver staining
87-96
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Christodoulides, Myron
(2019)
Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae.
In,
Methods in Molecular Biology.
(Methods in Molecular Biology, 1997)
Humana New York, .
(doi:10.1007/978-1-4939-9496-0_6).
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Abstract
Neisseria gonorrhoeae is a gram-negative obligate human pathogen that contains lipooligosaccharide (LOS) as a major constituent within the outer membrane. LOS plays a major role in pathogenesis by inducing host inflammatory responses and also enabling evasion of host innate immunity through sialylation. Epitopes within LOS are also potential vaccine candidates. In this chapter, we describe a general method based on the Westphal hot phenol extraction process to purify whole LOS from N. gonorrhoeae for structural analyses and for use in in vivo and in vitro biological assays.
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e-pub ahead of print date: 23 May 2019
Keywords:
Electrodialysis, Lipooligosaccharide, LOS, Phenol extraction, Silver staining
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Local EPrints ID: 431817
URI: http://eprints.soton.ac.uk/id/eprint/431817
ISSN: 1064-3745
PURE UUID: 891d209a-4c84-413a-b695-27f272e6524c
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Date deposited: 19 Jun 2019 16:30
Last modified: 16 Mar 2024 02:38
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