Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae

Christodoulides, Myron (2019) Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae. In, Methods in Molecular Biology. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-9496-0_6), 1997) Humana Press Inc., pp. 87-96. (doi:10.1007/978-1-4939-9496-0_6).

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Abstract

Neisseria gonorrhoeae is a gram-negative obligate human pathogen that contains lipooligosaccharide (LOS) as a major constituent within the outer membrane. LOS plays a major role in pathogenesis by inducing host inflammatory responses and also enabling evasion of host innate immunity through sialylation. Epitopes within LOS are also potential vaccine candidates. In this chapter, we describe a general method based on the Westphal hot phenol extraction process to purify whole LOS from N. gonorrhoeae for structural analyses and for use in in vivo and in vitro biological assays.

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e-pub ahead of print date: 23 May 2019

Keywords: Electrodialysis, Lipooligosaccharide, LOS, Phenol extraction, Silver staining

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Local EPrints ID: 431817

URI: https://eprints.soton.ac.uk/id/eprint/431817

DOI: doi:10.1007/978-1-4939-9496-0_6

ISSN: 1064-3745

PURE UUID: 891d209a-4c84-413a-b695-27f272e6524c

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Date deposited: 19 Jun 2019 16:30

Last modified: 19 Jun 2019 16:30

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Author: Myron Christodoulides

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