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Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae

Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae
Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae

Neisseria gonorrhoeae is a gram-negative obligate human pathogen that contains lipooligosaccharide (LOS) as a major constituent within the outer membrane. LOS plays a major role in pathogenesis by inducing host inflammatory responses and also enabling evasion of host innate immunity through sialylation. Epitopes within LOS are also potential vaccine candidates. In this chapter, we describe a general method based on the Westphal hot phenol extraction process to purify whole LOS from N. gonorrhoeae for structural analyses and for use in in vivo and in vitro biological assays.

Electrodialysis, Lipooligosaccharide, LOS, Phenol extraction, Silver staining
1064-3745
87-96
Humana Press Inc.
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078

Christodoulides, Myron (2019) Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae. In, Methods in Molecular Biology. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-9496-0_6), 1997) Humana Press Inc., pp. 87-96. (doi:10.1007/978-1-4939-9496-0_6).

Record type: Book Section

Abstract

Neisseria gonorrhoeae is a gram-negative obligate human pathogen that contains lipooligosaccharide (LOS) as a major constituent within the outer membrane. LOS plays a major role in pathogenesis by inducing host inflammatory responses and also enabling evasion of host innate immunity through sialylation. Epitopes within LOS are also potential vaccine candidates. In this chapter, we describe a general method based on the Westphal hot phenol extraction process to purify whole LOS from N. gonorrhoeae for structural analyses and for use in in vivo and in vitro biological assays.

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e-pub ahead of print date: 23 May 2019
Keywords: Electrodialysis, Lipooligosaccharide, LOS, Phenol extraction, Silver staining
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Identifiers

Local EPrints ID: 431817
URI: http://eprints.soton.ac.uk/id/eprint/431817
DOI: doi:10.1007/978-1-4939-9496-0_6
ISSN: 1064-3745
PURE UUID: 891d209a-4c84-413a-b695-27f272e6524c
ORCID for Myron Christodoulides: ORCID iD orcid.org/0000-0002-9663-4731

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Date deposited: 19 Jun 2019 16:30
Last modified: 18 Feb 2021 16:37

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Author: Myron Christodoulides ORCID iD

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