Basic methods for examining Neisseria gonorrhoeae interactions with host cells in vitro

Almonacid-Mendoza, Hannia Liliana and Christodoulides, Myron (2019) Basic methods for examining Neisseria gonorrhoeae interactions with host cells in vitro. In, Christodoulides, M. (ed.) Methods in Molecular Biology. (Methods in Molecular Biology, 1997) New York. Humana, pp. 281-299. (doi:10.1007/978-1-4939-9496-0_17).

Abstract

The obligate human pathogen Neisseria gonorrhoeae colonizes primarily the mucosal columnar epithelium of the male urethra and the female endocervix. In addition, gonococci can infect the anorectal, pharyngeal, and gingival mucosae and epithelial cells of the conjunctiva. More rarely, the organism can disseminate through the bloodstream, which can involve interactions with other host cell types, including blood vessel endothelial cells and innate immune cells such as dendritic cells, macrophages, and neutrophils. “Disseminated gonococcal infection” is a serious condition with various manifestations resulting from the seeding of organs and tissues with the pathogen. The host response to gonococcal infection is inflammatory. Knowledge of the biology of gonococcal interactions has been served well through the use of a wide variety of ex vivo models using host tissues and eukaryotic cell monocultures. These models have helped identify bacterial surface adhesins and invasins and the corresponding cell surface receptors that play roles in gonococcal pathogenesis. Furthermore, they have been useful for understanding virulence mechanisms as well as innate and adaptive immune responses. In this chapter, readers are provided with protocols for examining the basic interactions between gonococci and a representative human cell line.

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Contributors

Author: Myron Christodoulides

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