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Basic methods for examining Neisseria gonorrhoeae interactions with host cells in vitro

Basic methods for examining Neisseria gonorrhoeae interactions with host cells in vitro
Basic methods for examining Neisseria gonorrhoeae interactions with host cells in vitro

The obligate human pathogen Neisseria gonorrhoeae colonizes primarily the mucosal columnar epithelium of the male urethra and the female endocervix. In addition, gonococci can infect the anorectal, pharyngeal, and gingival mucosae and epithelial cells of the conjunctiva. More rarely, the organism can disseminate through the bloodstream, which can involve interactions with other host cell types, including blood vessel endothelial cells and innate immune cells such as dendritic cells, macrophages, and neutrophils. “Disseminated gonococcal infection” is a serious condition with various manifestations resulting from the seeding of organs and tissues with the pathogen. The host response to gonococcal infection is inflammatory. Knowledge of the biology of gonococcal interactions has been served well through the use of a wide variety of ex vivo models using host tissues and eukaryotic cell monocultures. These models have helped identify bacterial surface adhesins and invasins and the corresponding cell surface receptors that play roles in gonococcal pathogenesis. Furthermore, they have been useful for understanding virulence mechanisms as well as innate and adaptive immune responses. In this chapter, readers are provided with protocols for examining the basic interactions between gonococci and a representative human cell line.

Association, Cell culture, Cell death, Cytokine production, Invasion, Microscopy
1064-3745
281-299
Humana
Almonacid-Mendoza, Hannia Liliana
5ef98c79-11f8-48c8-a9dc-fdcbec45c96b
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Christodoulides, M.
Almonacid-Mendoza, Hannia Liliana
5ef98c79-11f8-48c8-a9dc-fdcbec45c96b
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Christodoulides, M.

Almonacid-Mendoza, Hannia Liliana and Christodoulides, Myron (2019) Basic methods for examining Neisseria gonorrhoeae interactions with host cells in vitro. In, Christodoulides, M. (ed.) Methods in Molecular Biology. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-9496-0_17), 1997) New York. Humana, pp. 281-299. (doi:10.1007/978-1-4939-9496-0_17).

Record type: Book Section

Abstract

The obligate human pathogen Neisseria gonorrhoeae colonizes primarily the mucosal columnar epithelium of the male urethra and the female endocervix. In addition, gonococci can infect the anorectal, pharyngeal, and gingival mucosae and epithelial cells of the conjunctiva. More rarely, the organism can disseminate through the bloodstream, which can involve interactions with other host cell types, including blood vessel endothelial cells and innate immune cells such as dendritic cells, macrophages, and neutrophils. “Disseminated gonococcal infection” is a serious condition with various manifestations resulting from the seeding of organs and tissues with the pathogen. The host response to gonococcal infection is inflammatory. Knowledge of the biology of gonococcal interactions has been served well through the use of a wide variety of ex vivo models using host tissues and eukaryotic cell monocultures. These models have helped identify bacterial surface adhesins and invasins and the corresponding cell surface receptors that play roles in gonococcal pathogenesis. Furthermore, they have been useful for understanding virulence mechanisms as well as innate and adaptive immune responses. In this chapter, readers are provided with protocols for examining the basic interactions between gonococci and a representative human cell line.

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More information

e-pub ahead of print date: 23 May 2019
Keywords: Association, Cell culture, Cell death, Cytokine production, Invasion, Microscopy

Identifiers

Local EPrints ID: 431870
URI: https://eprints.soton.ac.uk/id/eprint/431870
ISSN: 1064-3745
PURE UUID: e868257c-65fe-410c-8d1a-b5b119bfd08c

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Date deposited: 20 Jun 2019 16:30
Last modified: 20 Jun 2019 16:30

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