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Image‐based sorting and negative dielectrophoresis for high purity cell and particle separation

Image‐based sorting and negative dielectrophoresis for high purity cell and particle separation
Image‐based sorting and negative dielectrophoresis for high purity cell and particle separation
Microelectrode arrays are used to sort single fluorescently labeled cells and particles as they flow through a microfluidic channel using dielectrophoresis. Negative dielectrophoresis is used to create a “Dielectrophoretic virtual channel” that runs along the center of the microfluidic channel. By switching the polarity of the electrodes, the virtual channel can be dynamically reconfigured to direct particles along a different path. This is demonstrated by sorting particles into two microfluidic outlets, controlled by an automated system that interprets video data from a color camera and makes complex sorting decisions based on color, intensity, size, and shape. This enables the rejection of particle aggregates and other impurities, and the system is optimized to isolate high purity populations from a heterogeneous sample. Green beads are isolated from an excess of red beads with 100% purity at a rate of up to 0.9 particles per second, in addition application to the sorting of osteosarcoma and human bone marrow cells is evidenced. The extension of Dielectrophoretic Virtual Channels to an arbitrary number of sorting outputs is examined, with design, simulation, and experimental verification of two alternate geometries presented and compared.
1522-2683
Thomas, Rupert
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Mitchell, Peter D.
a6de2a77-02bc-4679-8717-4ad1153464bd
Oreffo, Richard O. C.
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Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Green, Nicolas
d9b47269-c426-41fd-a41d-5f4579faa581
Thomas, Rupert
32ee6e47-de4f-42fd-9af9-ab255cfc64a2
Mitchell, Peter D.
a6de2a77-02bc-4679-8717-4ad1153464bd
Oreffo, Richard O. C.
8f43702d-637e-45f9-b374-3d01c145b16c
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Green, Nicolas
d9b47269-c426-41fd-a41d-5f4579faa581

Thomas, Rupert, Mitchell, Peter D., Oreffo, Richard O. C., Morgan, Hywel and Green, Nicolas (2019) Image‐based sorting and negative dielectrophoresis for high purity cell and particle separation. Electrophoresis. (doi:10.1002/elps.201800489).

Record type: Article

Abstract

Microelectrode arrays are used to sort single fluorescently labeled cells and particles as they flow through a microfluidic channel using dielectrophoresis. Negative dielectrophoresis is used to create a “Dielectrophoretic virtual channel” that runs along the center of the microfluidic channel. By switching the polarity of the electrodes, the virtual channel can be dynamically reconfigured to direct particles along a different path. This is demonstrated by sorting particles into two microfluidic outlets, controlled by an automated system that interprets video data from a color camera and makes complex sorting decisions based on color, intensity, size, and shape. This enables the rejection of particle aggregates and other impurities, and the system is optimized to isolate high purity populations from a heterogeneous sample. Green beads are isolated from an excess of red beads with 100% purity at a rate of up to 0.9 particles per second, in addition application to the sorting of osteosarcoma and human bone marrow cells is evidenced. The extension of Dielectrophoretic Virtual Channels to an arbitrary number of sorting outputs is examined, with design, simulation, and experimental verification of two alternate geometries presented and compared.

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Thomas et al - Accepted Manuscript
Restricted to Repository staff only until 16 June 2020.
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More information

Accepted/In Press date: 21 May 2019
e-pub ahead of print date: 17 June 2019

Identifiers

Local EPrints ID: 432076
URI: https://eprints.soton.ac.uk/id/eprint/432076
ISSN: 1522-2683
PURE UUID: e17200f7-e779-4678-9f54-11ca130b1005
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Nicolas Green: ORCID iD orcid.org/0000-0001-9230-4455

Catalogue record

Date deposited: 01 Jul 2019 16:30
Last modified: 07 Aug 2019 00:43

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