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Effect of gestational oily fish intake on the risk of allergy in children may be influenced by FADS1/2, ELOVL5 expression and DNA methylation

Effect of gestational oily fish intake on the risk of allergy in children may be influenced by FADS1/2, ELOVL5 expression and DNA methylation
Effect of gestational oily fish intake on the risk of allergy in children may be influenced by FADS1/2, ELOVL5 expression and DNA methylation
Background: evidence suggests that prenatal exposure to n-3 long-chain polyunsaturated fatty acids (LCPUFA) reduces the incidence of allergic disease in children. LCPUFAs are produced from dietary precursors catalyzed by desaturases and elongases encoded by the FADS1/2 and ELOVL5 genes. DNA methylation regulates gene activity and fatty acid supplementation could alter DNA methylation (DNA-M) at these genes. We investigated whether DNA-M and expression of the FADS1/2 and ELOVL5 genes were associated with allergy in children and gestational fish intake. We studied 170 participants from the Isle of Wight 3rd Generation Cohort, UK. Phenotype data and exposure was assessed by questionnaires. Genome-wide DNA-M in cord blood samples was quantified using the Illumina Infinium HumanMethylation450 and EPIC Beadchips. Five SNPs (single-nucleotide polymorphisms) in the FADS gene cluster and one SNP in ELOVL5 were genotyped in offspring. FADS gene expression in offspring cord blood was determined. Results: gestational fish intake was significantly associated with increased methylation of cg12517394 (P = 0.049), which positively correlated with FADS1 mRNA levels (P = 0.021). ELOVL5 rs2397142 was significantly associated with eczema (P = 0.011) and methylation at cg11748354 and cg24524396 (P < 0.001 and P = 0.036, respectively). Gestational fish intake was strongly associated with elevated DNA-M at cg11748354 and cg24524396 (P = 0.029 and P = 0.002, respectively) and reduced ELOVL5 mRNA expression (P = 0.028). Conclusion: the association between induced FADS1/2 and ELOVL5 DNA-M and reduced gene expression due to gestational fish intake provide a mechanistic explanation of the previously observed association between maternal LCPUFA intake and allergy development in early childhood.
1555-8932
1-7
Losol, Purevsuren
85a3c8ff-a437-44d0-8472-9b090039cf63
Rezwan, Faisal I
203f8f38-1f5d-485b-ab11-c546b4276338
Patil, Veeresh, K
b898b9a7-db31-4c1c-b0f0-4165b3e4d29c
Venter, Carina
a9b7dd5e-b0cb-4068-be82-e15b587cc20b
Ewart, Susan
28667421-3cf7-43d7-b1c3-ca27564938f7
Zhang, Hongmei
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Arshad, Syed
917e246d-2e60-472f-8d30-94b01ef28958
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Losol, Purevsuren
85a3c8ff-a437-44d0-8472-9b090039cf63
Rezwan, Faisal I
203f8f38-1f5d-485b-ab11-c546b4276338
Patil, Veeresh, K
b898b9a7-db31-4c1c-b0f0-4165b3e4d29c
Venter, Carina
a9b7dd5e-b0cb-4068-be82-e15b587cc20b
Ewart, Susan
28667421-3cf7-43d7-b1c3-ca27564938f7
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Arshad, Syed
917e246d-2e60-472f-8d30-94b01ef28958
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a

Losol, Purevsuren, Rezwan, Faisal I, Patil, Veeresh, K, Venter, Carina, Ewart, Susan, Zhang, Hongmei, Arshad, Syed, Karmaus, Wilfried and Holloway, John (2019) Effect of gestational oily fish intake on the risk of allergy in children may be influenced by FADS1/2, ELOVL5 expression and DNA methylation. Genes & Nutrition, 14 (20), 1-7. (doi:10.1186/s12263-019-0644-8).

Record type: Article

Abstract

Background: evidence suggests that prenatal exposure to n-3 long-chain polyunsaturated fatty acids (LCPUFA) reduces the incidence of allergic disease in children. LCPUFAs are produced from dietary precursors catalyzed by desaturases and elongases encoded by the FADS1/2 and ELOVL5 genes. DNA methylation regulates gene activity and fatty acid supplementation could alter DNA methylation (DNA-M) at these genes. We investigated whether DNA-M and expression of the FADS1/2 and ELOVL5 genes were associated with allergy in children and gestational fish intake. We studied 170 participants from the Isle of Wight 3rd Generation Cohort, UK. Phenotype data and exposure was assessed by questionnaires. Genome-wide DNA-M in cord blood samples was quantified using the Illumina Infinium HumanMethylation450 and EPIC Beadchips. Five SNPs (single-nucleotide polymorphisms) in the FADS gene cluster and one SNP in ELOVL5 were genotyped in offspring. FADS gene expression in offspring cord blood was determined. Results: gestational fish intake was significantly associated with increased methylation of cg12517394 (P = 0.049), which positively correlated with FADS1 mRNA levels (P = 0.021). ELOVL5 rs2397142 was significantly associated with eczema (P = 0.011) and methylation at cg11748354 and cg24524396 (P < 0.001 and P = 0.036, respectively). Gestational fish intake was strongly associated with elevated DNA-M at cg11748354 and cg24524396 (P = 0.029 and P = 0.002, respectively) and reduced ELOVL5 mRNA expression (P = 0.028). Conclusion: the association between induced FADS1/2 and ELOVL5 DNA-M and reduced gene expression due to gestational fish intake provide a mechanistic explanation of the previously observed association between maternal LCPUFA intake and allergy development in early childhood.

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Losol 2019 Article Effect Of Gestational Oily Fish - Version of Record
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Accepted/In Press date: 5 June 2019
Published date: 18 June 2019

Identifiers

Local EPrints ID: 432209
URI: http://eprints.soton.ac.uk/id/eprint/432209
ISSN: 1555-8932
PURE UUID: 5a584509-ed0d-4360-a445-65224755d302
ORCID for Faisal I Rezwan: ORCID iD orcid.org/0000-0001-9921-222X
ORCID for John Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 04 Jul 2019 16:30
Last modified: 26 Nov 2021 03:00

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Contributors

Author: Purevsuren Losol
Author: Faisal I Rezwan ORCID iD
Author: Veeresh, K Patil
Author: Carina Venter
Author: Susan Ewart
Author: Hongmei Zhang
Author: Syed Arshad
Author: Wilfried Karmaus
Author: John Holloway ORCID iD

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