Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” (Water Research (2017) 126 (101–110), (S0043135417306942), (10.1016/j.watres.2017.08.032))

Walker, David I., McQuillan, Jonathan, Taiwo, Michael, Parks, Rachel, Stenton, Craig A., Morgan, H., Mowlem, Matthew C. and Lees, David N. (2019) Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” (Water Research (2017) 126 (101–110), (S0043135417306942), (10.1016/j.watres.2017.08.032)). Water Research, 161, 652. (doi:10.1016/j.watres.2019.06.070).

Abstract

It has been brought to our attention that there is an error in the Methods, section 2.6 “Real-time PCR”. The article as published states “Each reaction contained 12.5 μl of IQ SYBR Supermix con- taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 ml of each primer (final concentration 5 mM) and 1 ml of template DNA at 100 ng/μl; the final volume was 25 μl.”. However this should be “Each reaction contained 12.5 μl of IQ SYBR Supermix con- taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 μl of each primer (final concentration 0.4 μM) and 1 μl of template DNA at 100 ng/μl; the final volume was 25 μl.”

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Contributors

Author: H. Morgan

Author: Matthew C. Mowlem

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