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Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” (Water Research (2017) 126 (101–110), (S0043135417306942), (10.1016/j.watres.2017.08.032))

Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” (Water Research (2017) 126 (101–110), (S0043135417306942), (10.1016/j.watres.2017.08.032))
Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” (Water Research (2017) 126 (101–110), (S0043135417306942), (10.1016/j.watres.2017.08.032))

It has been brought to our attention that there is an error in the Methods, section 2.6 “Real-time PCR”. The article as published states “Each reaction contained 12.5 μl of IQ SYBR Supermix con- taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 ml of each primer (final concentration 5 mM) and 1 ml of template DNA at 100 ng/μl; the final volume was 25 μl.”. However this should be “Each reaction contained 12.5 μl of IQ SYBR Supermix con- taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 μl of each primer (final concentration 0.4 μM) and 1 μl of template DNA at 100 ng/μl; the final volume was 25 μl.”

0043-1354
652
Walker, David I.
f80437ee-76da-496e-bf2a-ac1ac170159f
McQuillan, Jonathan
697cdf72-f353-4779-b64a-45494f29772f
Taiwo, Michael
3ac7811b-4d5d-487b-9a49-4453595c2e61
Parks, Rachel
342f6345-2cbb-4480-921f-97e5d8a6265d
Stenton, Craig A.
e7880000-c3b9-4c7d-8e67-26483e5386f9
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Mowlem, Matthew C.
6f633ca2-298f-48ee-a025-ce52dd62124f
Lees, David N.
276df848-c299-43bb-a340-43ebd8604541
Walker, David I.
f80437ee-76da-496e-bf2a-ac1ac170159f
McQuillan, Jonathan
697cdf72-f353-4779-b64a-45494f29772f
Taiwo, Michael
3ac7811b-4d5d-487b-9a49-4453595c2e61
Parks, Rachel
342f6345-2cbb-4480-921f-97e5d8a6265d
Stenton, Craig A.
e7880000-c3b9-4c7d-8e67-26483e5386f9
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Mowlem, Matthew C.
6f633ca2-298f-48ee-a025-ce52dd62124f
Lees, David N.
276df848-c299-43bb-a340-43ebd8604541

Walker, David I., McQuillan, Jonathan, Taiwo, Michael, Parks, Rachel, Stenton, Craig A., Morgan, H., Mowlem, Matthew C. and Lees, David N. (2019) Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” (Water Research (2017) 126 (101–110), (S0043135417306942), (10.1016/j.watres.2017.08.032)). Water Research, 161, 652. (doi:10.1016/j.watres.2019.06.070).

Record type: Letter

Abstract

It has been brought to our attention that there is an error in the Methods, section 2.6 “Real-time PCR”. The article as published states “Each reaction contained 12.5 μl of IQ SYBR Supermix con- taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 ml of each primer (final concentration 5 mM) and 1 ml of template DNA at 100 ng/μl; the final volume was 25 μl.”. However this should be “Each reaction contained 12.5 μl of IQ SYBR Supermix con- taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 μl of each primer (final concentration 0.4 μM) and 1 μl of template DNA at 100 ng/μl; the final volume was 25 μl.”

Full text not available from this repository.

More information

e-pub ahead of print date: 4 July 2019
Published date: 15 September 2019

Identifiers

Local EPrints ID: 432380
URI: https://eprints.soton.ac.uk/id/eprint/432380
ISSN: 0043-1354
PURE UUID: 096c965b-5298-45b6-b51e-c0890d3a9f3d
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 12 Jul 2019 16:30
Last modified: 17 Sep 2019 00:52

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