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Waveguide absorption spectroscopy of bovine serum albumin in the mid-infrared fingerprint region

Waveguide absorption spectroscopy of bovine serum albumin in the mid-infrared fingerprint region
Waveguide absorption spectroscopy of bovine serum albumin in the mid-infrared fingerprint region
Protein sensing in biological fluids provides important information to diagnose many clinically relevant diseases. Mid-infrared (MIR) absorption spectroscopy of Bovine Serum Albumin (BSA) is experimentally demonstrated on a germanium on silicon (GOS) waveguide in the 1900-1000 cm-1 (5.3-10.0 µm) region of the MIR. GOS waveguides were shown to guide light up to a wavelength of 12.9 µm. The waveguide absorption spectrum of water, showing molecular bending vibrations, was obtained experimentally and compared with a theoretical model showing good agreement. Measurement of a concentration series of BSA protein in phosphate buffered saline (PBS) from 0.1 mg/ml to 100mg/ml was performed on the waveguide using filter paper as a flow strip and the amide I, II and III peaks were observed and quantified.
mid-infrared, waveguide, germanium, absorption spectroscopy, protein, amide
2379-3694
1749-1753
Mittal, Vinita
fd5ee9dd-7770-416f-8f47-50ca158b39b0
Nedeljković, Milos
b64e21c2-1b95-479d-a35c-3456dff8c796
Carpenter, Lewis Glynn
0daa548e-0d42-4b06-b914-45bfbec41759
Khokhar, Ali
2eedd1cc-8ac5-4f8e-be25-930bd3eae396
Chong, Harold
795aa67f-29e5-480f-b1bc-9bd5c0d558e1
Mashanovich, Goran
c806e262-af80-4836-b96f-319425060051
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Wilkinson, James S
73483cf3-d9f2-4688-9b09-1c84257884ca
Mittal, Vinita
fd5ee9dd-7770-416f-8f47-50ca158b39b0
Nedeljković, Milos
b64e21c2-1b95-479d-a35c-3456dff8c796
Carpenter, Lewis Glynn
0daa548e-0d42-4b06-b914-45bfbec41759
Khokhar, Ali
2eedd1cc-8ac5-4f8e-be25-930bd3eae396
Chong, Harold
795aa67f-29e5-480f-b1bc-9bd5c0d558e1
Mashanovich, Goran
c806e262-af80-4836-b96f-319425060051
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Wilkinson, James S
73483cf3-d9f2-4688-9b09-1c84257884ca

Mittal, Vinita, Nedeljković, Milos, Carpenter, Lewis Glynn, Khokhar, Ali, Chong, Harold, Mashanovich, Goran, Bartlett, Philip N. and Wilkinson, James S (2019) Waveguide absorption spectroscopy of bovine serum albumin in the mid-infrared fingerprint region. ACS Sensors, 4 (7), 1749-1753. (doi:10.1021/acssensors.9b00215).

Record type: Article

Abstract

Protein sensing in biological fluids provides important information to diagnose many clinically relevant diseases. Mid-infrared (MIR) absorption spectroscopy of Bovine Serum Albumin (BSA) is experimentally demonstrated on a germanium on silicon (GOS) waveguide in the 1900-1000 cm-1 (5.3-10.0 µm) region of the MIR. GOS waveguides were shown to guide light up to a wavelength of 12.9 µm. The waveguide absorption spectrum of water, showing molecular bending vibrations, was obtained experimentally and compared with a theoretical model showing good agreement. Measurement of a concentration series of BSA protein in phosphate buffered saline (PBS) from 0.1 mg/ml to 100mg/ml was performed on the waveguide using filter paper as a flow strip and the amide I, II and III peaks were observed and quantified.

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Waveguide Absorption Spectroscopy of Bovine Serum Albumin in the Mid-Infrared Fingerprint Region - Accepted Manuscript
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Supplementary Information: Waveguide Absorption Spectroscopy of Bovine Serum Albumin in the Mid-Infrared Fingerprint Region - Accepted Manuscript
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Submitted date: 29 May 2019
Accepted/In Press date: 2 July 2019
e-pub ahead of print date: 5 July 2019
Published date: 26 July 2019
Keywords: mid-infrared, waveguide, germanium, absorption spectroscopy, protein, amide

Identifiers

Local EPrints ID: 432545
URI: https://eprints.soton.ac.uk/id/eprint/432545
ISSN: 2379-3694
PURE UUID: 96873a00-fd24-448d-8716-f52f013ca9c4
ORCID for Vinita Mittal: ORCID iD orcid.org/0000-0003-4836-5327
ORCID for Milos Nedeljković: ORCID iD orcid.org/0000-0002-9170-7911
ORCID for Harold Chong: ORCID iD orcid.org/0000-0002-7110-5761
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900
ORCID for James S Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 17 Jul 2019 16:33
Last modified: 12 Oct 2019 04:01

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