Mittal, Vinita, Nedeljković, Miloš, Carpenter, Lewis Glynn, Khokhar, Ali, Chong, Harold, Mashanovich, Goran, Bartlett, Philip N. and Wilkinson, James S (2019) Waveguide absorption spectroscopy of bovine serum albumin in the mid-infrared fingerprint region. ACS Sensors, 4 (7), 1749-1753. (doi:10.1021/acssensors.9b00215).
Abstract
Protein sensing in biological fluids provides important information to diagnose many clinically relevant diseases. Mid-infrared (MIR) absorption spectroscopy of Bovine Serum Albumin (BSA) is experimentally demonstrated on a germanium on silicon (GOS) waveguide in the 1900-1000 cm-1 (5.3-10.0 µm) region of the MIR. GOS waveguides were shown to guide light up to a wavelength of 12.9 µm. The waveguide absorption spectrum of water, showing molecular bending vibrations, was obtained experimentally and compared with a theoretical model showing good agreement. Measurement of a concentration series of BSA protein in phosphate buffered saline (PBS) from 0.1 mg/ml to 100mg/ml was performed on the waveguide using filter paper as a flow strip and the amide I, II and III peaks were observed and quantified.
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- Current Faculties > Faculty of Engineering and Physical Sciences > School of Chemistry > Electrochemistry
School of Chemistry > Electrochemistry - Current Faculties > Faculty of Engineering and Physical Sciences > Zepler Institute for Photonics and Nanoelectronics > Nanophotonics Group > Photonic Systems Circuits and Sensors Group
Zepler Institute for Photonics and Nanoelectronics > Nanophotonics Group > Photonic Systems Circuits and Sensors Group - Faculties (pre 2018 reorg) > Faculty of Natural and Environmental Sciences (pre 2018 reorg) > Institute for Life Sciences (pre 2018 reorg)
Current Faculties > Faculty of Environmental and Life Sciences > Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg) - Current Faculties > Faculty of Engineering and Physical Sciences > School of Electronics and Computer Science > Sustainable Electronic Technologies
School of Electronics and Computer Science > Sustainable Electronic Technologies
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