The University of Southampton
University of Southampton Institutional Repository

Spatio-temporal properties of neurotrophin signalling in tauopathy

Spatio-temporal properties of neurotrophin signalling in tauopathy
Spatio-temporal properties of neurotrophin signalling in tauopathy
Neuronal cells form a highly organised neuronal network that allows us to function in everyday life. In order to maintain a healthy network, neuronal brain cells synthesise and secrete growth factors. Brain-derived neurotrophic growth factor (BDNF) is one of the growth factors widely expressed in the brain. Its role is to induce cell growth and strengthen connections between neuronal cells.

A significant decrease in the amount of BDNF has been detected in the brains of individuals who have suffered from neurodegenerative diseases such as Alzheimer’s disease, compared to healthy brains. Upregulating BDNF could be a potential therapeutic strategy to strengthen the connections of vulnerable neurons before the onset of neuronal death.

My PhD project looks to investigate to which degree BDNF-TrkB signalling is intact, both locally and over long-distances in neuronal brain cells that express a disease relevant mutation and whether these signals can support the vulnerable neuronal brain cells. In order to investigate this, I expressed a tauopathy related mutation (TauP301L) in embryonic mouse neuronal brain cells. I have observed disruption in BDNF transport and a significant change in the expression of its receptor, TrkB, at distal axons on TauP301L-expressing neurons. To assess BDNF signal relay, I have set-up a microfluidic platform that allows us to investigate both local and long-distance intracellular signalling of BDNF. I have seen a disruption in BDNF signalling in TauP301L-expressing neurons. This research gives us insight into whether growth factors are feasible for therapeutic strategies.
University of Southampton
Patel, Prutha
fb6d4749-ff22-420b-a1fa-b6a6fcaabad8
Patel, Prutha
fb6d4749-ff22-420b-a1fa-b6a6fcaabad8
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1

Patel, Prutha (2019) Spatio-temporal properties of neurotrophin signalling in tauopathy. University of Southampton, Doctoral Thesis, 223pp.

Record type: Thesis (Doctoral)

Abstract

Neuronal cells form a highly organised neuronal network that allows us to function in everyday life. In order to maintain a healthy network, neuronal brain cells synthesise and secrete growth factors. Brain-derived neurotrophic growth factor (BDNF) is one of the growth factors widely expressed in the brain. Its role is to induce cell growth and strengthen connections between neuronal cells.

A significant decrease in the amount of BDNF has been detected in the brains of individuals who have suffered from neurodegenerative diseases such as Alzheimer’s disease, compared to healthy brains. Upregulating BDNF could be a potential therapeutic strategy to strengthen the connections of vulnerable neurons before the onset of neuronal death.

My PhD project looks to investigate to which degree BDNF-TrkB signalling is intact, both locally and over long-distances in neuronal brain cells that express a disease relevant mutation and whether these signals can support the vulnerable neuronal brain cells. In order to investigate this, I expressed a tauopathy related mutation (TauP301L) in embryonic mouse neuronal brain cells. I have observed disruption in BDNF transport and a significant change in the expression of its receptor, TrkB, at distal axons on TauP301L-expressing neurons. To assess BDNF signal relay, I have set-up a microfluidic platform that allows us to investigate both local and long-distance intracellular signalling of BDNF. I have seen a disruption in BDNF signalling in TauP301L-expressing neurons. This research gives us insight into whether growth factors are feasible for therapeutic strategies.

Text
Prutha Patel FINAL Thesis - Version of Record
Available under License University of Southampton Thesis Licence.
Download (41MB)

More information

Published date: June 2019

Identifiers

Local EPrints ID: 432569
URI: http://eprints.soton.ac.uk/id/eprint/432569
PURE UUID: f23cbc30-ed92-4790-b2ba-2c412148e066
ORCID for Katrin Deinhardt: ORCID iD orcid.org/0000-0002-6473-5298

Catalogue record

Date deposited: 18 Jul 2019 16:31
Last modified: 26 Jun 2020 04:01

Export record

Contributors

Author: Prutha Patel
Thesis advisor: Katrin Deinhardt ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×