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Engineering multi-layered tissue constructs using acoustic levitation

Engineering multi-layered tissue constructs using acoustic levitation
Engineering multi-layered tissue constructs using acoustic levitation

Engineering tissue structures that mimic those found in vivo remains a challenge for modern biology. We demonstrate a new technique for engineering composite structures of cells comprising layers of heterogeneous cell types. An acoustofluidic bioreactor is used to assemble epithelial cells into a sheet-like structure. On transferring these cell sheets to a confluent layer of fibroblasts, the epithelial cells cover the fibroblast surface by collective migration maintaining distinct epithelial and fibroblast cell layers. The collective behaviour of the epithelium is dependent on the formation of cell-cell junctions during levitation and contrasts with the behaviour of mono-dispersed epithelial cells where cell-matrix interactions dominate and hinder formation of discrete cell layers. The multilayered tissue model is shown to form a polarised epithelial barrier and respond to apical challenge. The method is useful for engineering a wide range of layered tissue types and mechanistic studies on collective cell migration.

2045-2322
Tait, Angela
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Glynne-Jones, Peter
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Hill, Alison R.
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Smart, David E.
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Blume, Cornelia
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Hammarstrom, Bjorn
e68f865f-bb5e-4170-bc91-cf7b4ea60ba3
Fisher, Adam L.
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Grossel, Martin C.
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Swindle, Emily J.
fe393c7a-a513-4de4-b02e-27369bd7e84f
Hill, Martyn
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Davies, Donna E.
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Tait, Angela
91d3e403-54d5-4efd-94c5-8b476e6af283
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Hill, Alison R.
de7a9d4f-7c5c-440c-9619-5b390f6347fd
Smart, David E.
3468eafa-5280-40a1-9fda-1bdc07dd914f
Blume, Cornelia
aa391c64-8718-4238-906b-d6bb1551a07b
Hammarstrom, Bjorn
e68f865f-bb5e-4170-bc91-cf7b4ea60ba3
Fisher, Adam L.
c8608350-dbc2-4571-8e45-7261b58df8b8
Grossel, Martin C.
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Swindle, Emily J.
fe393c7a-a513-4de4-b02e-27369bd7e84f
Hill, Martyn
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Davies, Donna E.
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Tait, Angela, Glynne-Jones, Peter, Hill, Alison R., Smart, David E., Blume, Cornelia, Hammarstrom, Bjorn, Fisher, Adam L., Grossel, Martin C., Swindle, Emily J., Hill, Martyn and Davies, Donna E. (2019) Engineering multi-layered tissue constructs using acoustic levitation. Scientific Reports, 9 (1), [9789]. (doi:10.1038/s41598-019-46201-z).

Record type: Article

Abstract

Engineering tissue structures that mimic those found in vivo remains a challenge for modern biology. We demonstrate a new technique for engineering composite structures of cells comprising layers of heterogeneous cell types. An acoustofluidic bioreactor is used to assemble epithelial cells into a sheet-like structure. On transferring these cell sheets to a confluent layer of fibroblasts, the epithelial cells cover the fibroblast surface by collective migration maintaining distinct epithelial and fibroblast cell layers. The collective behaviour of the epithelium is dependent on the formation of cell-cell junctions during levitation and contrasts with the behaviour of mono-dispersed epithelial cells where cell-matrix interactions dominate and hinder formation of discrete cell layers. The multilayered tissue model is shown to form a polarised epithelial barrier and respond to apical challenge. The method is useful for engineering a wide range of layered tissue types and mechanistic studies on collective cell migration.

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More information

Accepted/In Press date: 14 June 2019
e-pub ahead of print date: 5 July 2019
Published date: 1 December 2019

Identifiers

Local EPrints ID: 432612
URI: http://eprints.soton.ac.uk/id/eprint/432612
ISSN: 2045-2322
PURE UUID: ef749565-1298-435d-8b35-c239b91aa48a
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for Alison R. Hill: ORCID iD orcid.org/0000-0001-5397-873X
ORCID for Cornelia Blume: ORCID iD orcid.org/0000-0001-6133-7318
ORCID for Martin C. Grossel: ORCID iD orcid.org/0000-0001-7469-6854
ORCID for Emily J. Swindle: ORCID iD orcid.org/0000-0003-3644-7747
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for Donna E. Davies: ORCID iD orcid.org/0000-0002-5117-2991

Catalogue record

Date deposited: 22 Jul 2019 16:30
Last modified: 18 Feb 2021 17:31

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