Tait, Angela, Glynne-Jones, Peter, Hill, Alison R., Smart, David E., Blume, Cornelia, Hammarstrom, Bjorn, Fisher, Adam L., Grossel, Martin C., Swindle, Emily J., Hill, Martyn and Davies, Donna E. (2019) Engineering multi-layered tissue constructs using acoustic levitation. Scientific Reports, 9 (1), [9789]. (doi:10.1038/s41598-019-46201-z).
Abstract
Engineering tissue structures that mimic those found in vivo remains a challenge for modern biology. We demonstrate a new technique for engineering composite structures of cells comprising layers of heterogeneous cell types. An acoustofluidic bioreactor is used to assemble epithelial cells into a sheet-like structure. On transferring these cell sheets to a confluent layer of fibroblasts, the epithelial cells cover the fibroblast surface by collective migration maintaining distinct epithelial and fibroblast cell layers. The collective behaviour of the epithelium is dependent on the formation of cell-cell junctions during levitation and contrasts with the behaviour of mono-dispersed epithelial cells where cell-matrix interactions dominate and hinder formation of discrete cell layers. The multilayered tissue model is shown to form a polarised epithelial barrier and respond to apical challenge. The method is useful for engineering a wide range of layered tissue types and mechanistic studies on collective cell migration.
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- Current Faculties > Faculty of Medicine > NIHR Southampton Biomedical Research Centre
NIHR Southampton Biomedical Research Centre - Faculties (pre 2018 reorg) > Faculty of Engineering and the Environment (pre 2018 reorg) > Mechanical Engineering (pre 2018 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > School of Engineering > Mechanical Engineering > Mechanical Engineering (pre 2018 reorg)
Mechanical Engineering > Mechanical Engineering (pre 2018 reorg) - Faculties (pre 2018 reorg) > Faculty of Natural and Environmental Sciences (pre 2018 reorg) > Institute for Life Sciences (pre 2018 reorg)
Current Faculties > Faculty of Environmental and Life Sciences > Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
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