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UBE2S is associated with malignant characteristics of breast cancer cells

UBE2S is associated with malignant characteristics of breast cancer cells
UBE2S is associated with malignant characteristics of breast cancer cells
Ubiquitination is essential for various biological processes, such as signal transduction, intracellular trafficking, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskeleton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.
1010-4283
763-772
Akter, Khondker Ayesha
158fdaf8-1b26-4513-b123-f0e1729578b0
Hyodo, Toshinori
d7721c0c-bfff-4899-b244-2686bfadd145
Asano, Eri
ebc3d41e-dbcb-4d22-94a6-2b5818672545
Sato, Naoki
6e38b2e2-cc47-426a-bd8b-c36869a9a9f2
Mansour, Mohammed
03d901e1-959e-4c7d-acc6-33c6a4b129f7
Ito, Satoko
1c0f236c-2128-41d0-8a88-00aaba826efb
Hamaguchi, Michinari
557c7853-d4ce-49b2-9069-6962047ed541
Senga, Takeshi
3d9abecc-8bf9-4c1c-a64a-f598d5767cfc
Akter, Khondker Ayesha
158fdaf8-1b26-4513-b123-f0e1729578b0
Hyodo, Toshinori
d7721c0c-bfff-4899-b244-2686bfadd145
Asano, Eri
ebc3d41e-dbcb-4d22-94a6-2b5818672545
Sato, Naoki
6e38b2e2-cc47-426a-bd8b-c36869a9a9f2
Mansour, Mohammed
03d901e1-959e-4c7d-acc6-33c6a4b129f7
Ito, Satoko
1c0f236c-2128-41d0-8a88-00aaba826efb
Hamaguchi, Michinari
557c7853-d4ce-49b2-9069-6962047ed541
Senga, Takeshi
3d9abecc-8bf9-4c1c-a64a-f598d5767cfc

Akter, Khondker Ayesha, Hyodo, Toshinori, Asano, Eri, Sato, Naoki, Mansour, Mohammed, Ito, Satoko, Hamaguchi, Michinari and Senga, Takeshi (2016) UBE2S is associated with malignant characteristics of breast cancer cells. Tumor Biology, 763-772. (doi:10.1007/s13277-015-3863-7).

Record type: Article

Abstract

Ubiquitination is essential for various biological processes, such as signal transduction, intracellular trafficking, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskeleton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.

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More information

Accepted/In Press date: 29 July 2015
e-pub ahead of print date: 5 August 2015
Published date: January 2016

Identifiers

Local EPrints ID: 432623
URI: http://eprints.soton.ac.uk/id/eprint/432623
ISSN: 1010-4283
PURE UUID: cd6bed22-aa9f-40c7-992e-942070b7b0b3

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Date deposited: 22 Jul 2019 16:30
Last modified: 22 Jul 2019 16:30

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Contributors

Author: Khondker Ayesha Akter
Author: Toshinori Hyodo
Author: Eri Asano
Author: Naoki Sato
Author: Satoko Ito
Author: Michinari Hamaguchi
Author: Takeshi Senga

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