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A primate-specific short GluN2A-NMDA receptor isoform is expressed in the human brain

A primate-specific short GluN2A-NMDA receptor isoform is expressed in the human brain
A primate-specific short GluN2A-NMDA receptor isoform is expressed in the human brain
Glutamate receptors of the N-methyl-D-aspartate (NMDA) family are coincident detectors of pre- and postsynaptic activity, allowing Ca2+ influx into neurons. These properties are central to neurological disease mechanisms and are proposed to be the basis of associative learning and memory. In addition to the well-characterised canonical GluN2A NMDAR isoform, large-scale open reading frames in human tissues had suggested the expression of a primate-specific short GluN2A isoform referred to as GluN2A-S. Here, we confirm the expression of both GluN2A transcripts in human and primate but not rodent brain tissue, and show that they are translated to two corresponding GluN2A proteins present in human brain. Furthermore, we demonstrate that recombinant GluN2A-S co-assembles with the obligatory NMDAR subunit GluN1 to form functional NMDA receptors. These findings suggest a more complex NMDAR repertoire in human brain than previously thought.
1756-6606
1-8
Vargas-Caballero, Mariana
de2178ac-77fd-4748-9fe5-109ab8ad93e1
Warming, Hannah, Kate
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Pegasiou, Chrysia-Maria
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Pitera, Aleksandra, Paulina
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Kariis, Hanna
45afcebb-fd3f-45ca-af2c-ec7c3c6b0ff3
Houghton, Steven
b7d64445-1c7a-4b7e-87e4-98978460fc6e
Kurbatskaya, Ksenia
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Ahmed, Aminul
5f4bb605-0cee-47c0-8172-8c87c1ef8ba4
Grundy, Paul
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Vajramani, Girish V.
c3065130-3c48-45fb-b771-299ed0c66fa9
Bulters, Diederik
d6f9644a-a32f-45d8-b5ed-be54486ec21d
Altafaj, Xavier
07b9f486-b379-45d0-ae8c-8a02551884f0
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Vargas-Caballero, Mariana
de2178ac-77fd-4748-9fe5-109ab8ad93e1
Warming, Hannah, Kate
52e41d3f-0cf4-440f-b0c9-cec47ed3b23a
Pegasiou, Chrysia-Maria
70534d16-7533-40c3-b017-2840366f6618
Pitera, Aleksandra, Paulina
6cf28d67-483b-494b-8323-c01a64c24f71
Kariis, Hanna
45afcebb-fd3f-45ca-af2c-ec7c3c6b0ff3
Houghton, Steven
b7d64445-1c7a-4b7e-87e4-98978460fc6e
Kurbatskaya, Ksenia
11d25414-e10d-413a-aaf3-fb6b6c2cf890
Ahmed, Aminul
5f4bb605-0cee-47c0-8172-8c87c1ef8ba4
Grundy, Paul
0ceddba7-59f8-469f-8b50-4d08936e92a0
Vajramani, Girish V.
c3065130-3c48-45fb-b771-299ed0c66fa9
Bulters, Diederik
d6f9644a-a32f-45d8-b5ed-be54486ec21d
Altafaj, Xavier
07b9f486-b379-45d0-ae8c-8a02551884f0
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1

Vargas-Caballero, Mariana, Warming, Hannah, Kate, Pegasiou, Chrysia-Maria, Pitera, Aleksandra, Paulina, Kariis, Hanna, Houghton, Steven, Kurbatskaya, Ksenia, Ahmed, Aminul, Grundy, Paul, Vajramani, Girish V., Bulters, Diederik, Altafaj, Xavier and Deinhardt, Katrin (2019) A primate-specific short GluN2A-NMDA receptor isoform is expressed in the human brain. Molecular Brain, 12 (1), 1-8, [64]. (doi:10.1186/s13041-019-0485-9).

Record type: Article

Abstract

Glutamate receptors of the N-methyl-D-aspartate (NMDA) family are coincident detectors of pre- and postsynaptic activity, allowing Ca2+ influx into neurons. These properties are central to neurological disease mechanisms and are proposed to be the basis of associative learning and memory. In addition to the well-characterised canonical GluN2A NMDAR isoform, large-scale open reading frames in human tissues had suggested the expression of a primate-specific short GluN2A isoform referred to as GluN2A-S. Here, we confirm the expression of both GluN2A transcripts in human and primate but not rodent brain tissue, and show that they are translated to two corresponding GluN2A proteins present in human brain. Furthermore, we demonstrate that recombinant GluN2A-S co-assembles with the obligatory NMDAR subunit GluN1 to form functional NMDA receptors. These findings suggest a more complex NMDAR repertoire in human brain than previously thought.

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Accepted/In Press date: 21 June 2019
Published date: 4 July 2019

Identifiers

Local EPrints ID: 432715
URI: http://eprints.soton.ac.uk/id/eprint/432715
ISSN: 1756-6606
PURE UUID: ecfcd679-9976-422e-a5a9-068ef2f8d93d
ORCID for Mariana Vargas-Caballero: ORCID iD orcid.org/0000-0003-2326-4001
ORCID for Katrin Deinhardt: ORCID iD orcid.org/0000-0002-6473-5298

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Date deposited: 24 Jul 2019 16:30
Last modified: 18 Feb 2021 17:20

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Contributors

Author: Chrysia-Maria Pegasiou
Author: Aleksandra, Paulina Pitera
Author: Hanna Kariis
Author: Steven Houghton
Author: Aminul Ahmed
Author: Paul Grundy
Author: Girish V. Vajramani
Author: Diederik Bulters
Author: Xavier Altafaj

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