Khedr, M.E.M.S., Abdelmotelb, Ahmed, Bedwell, Tom A., Abu-Hilal, Mohammed and Khakoo, Salim (2016) Vasoactive intestinal peptide (VIP) induces proliferation of human hepatocytes. Faculty of Medicine Research Conference, University of Southampton, United Kingdom. 23 Jun 2016.
Abstract
Background: VIP is a gastrointestinal peptide hormone which regulates cell proliferation and differentiation in many cell types. In vitro, hepatocytes have limited viability and proliferative capacity. Although several growth factors such as epidermal growth factor (EGF) have been studied, role of VIP is still unclear. We have investigated the effect of VIP on proliferation of human hepatocytes.
METHODS: Human hepatocytes were isolated by two-steps collagenase protocol from liver specimens obtained from patients undergoing liver surgery. Cells were grown in Williams’ E media on collagen coated culture plate. Following 4 to 6 hours of cell plating, treatment with VIP or EGF was started and continued for 3 or 5 days. DNA replication was investigated by measuring Bromodeoxyuridine, BrdU incorporation using immunoflorescent staining. In parallel experiments; 1, 3 or 5 days total RNA was isolated and RT–PCR was performed. In the cell culture supernatant, urea and albumin concentrations were detected.
RESULTS: VIP was able to increase total number of hepatocytes and number of proliferating cells in a dose dependent manner markedly at day 3 of treatment. Treatment with VIP was associated with an increase in mRNA expression of ki-67 and H3 genes in a dose dependent process. Although mRNA expression of albumin gene was increased significantly with EGF, no marked alteration was found with VIP treatment. With increasing time, addition of VIP was associated with a decrease in albumin and urea secretion from liver cells.
CONCLUSIONS: VIP was able to induce proliferation of human hepatocytes but with little effects on hepatocytes differentiation.
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- Current Faculties > Faculty of Medicine > Clinical and Experimental Sciences > Tissue Infection and Repair
Clinical and Experimental Sciences > Tissue Infection and Repair - Faculties (pre 2011 reorg) > Faculty of Medicine Health & Life Sciences (pre 2011 reorg) > Medicine (pre 2011 reorg)
Faculties (pre 2018 reorg) > Faculty of Medicine (pre 2018 reorg) > Medicine (pre 2011 reorg) - Faculties (pre 2018 reorg) > Faculty of Natural and Environmental Sciences (pre 2018 reorg) > Institute for Life Sciences (pre 2018 reorg)
Current Faculties > Faculty of Environmental and Life Sciences > Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg) - Faculties (pre 2018 reorg) > Faculty of Medicine (pre 2018 reorg)
- Current Faculties > Faculty of Medicine > Clinical and Experimental Sciences
Clinical and Experimental Sciences
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