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Vasoactive intestinal peptide (VIP) induces proliferation of human hepatocytes

Vasoactive intestinal peptide (VIP) induces proliferation of human hepatocytes
Vasoactive intestinal peptide (VIP) induces proliferation of human hepatocytes
Background: VIP is a gastrointestinal peptide hormone which regulates cell proliferation and differentiation in many cell types. In vitro, hepatocytes have limited viability and proliferative capacity. Although several growth factors such as epidermal growth factor (EGF) have been studied, role of VIP is still unclear. We have investigated the effect of VIP on proliferation of human hepatocytes.
METHODS: Human hepatocytes were isolated by two-steps collagenase protocol from liver specimens obtained from patients undergoing liver surgery. Cells were grown in Williams’ E media on collagen coated culture plate. Following 4 to 6 hours of cell plating, treatment with VIP or EGF was started and continued for 3 or 5 days. DNA replication was investigated by measuring Bromodeoxyuridine, BrdU incorporation using immunoflorescent staining. In parallel experiments; 1, 3 or 5 days total RNA was isolated and RT–PCR was performed. In the cell culture supernatant, urea and albumin concentrations were detected.
RESULTS: VIP was able to increase total number of hepatocytes and number of proliferating cells in a dose dependent manner markedly at day 3 of treatment. Treatment with VIP was associated with an increase in mRNA expression of ki-67 and H3 genes in a dose dependent process. Although mRNA expression of albumin gene was increased significantly with EGF, no marked alteration was found with VIP treatment. With increasing time, addition of VIP was associated with a decrease in albumin and urea secretion from liver cells.
CONCLUSIONS: VIP was able to induce proliferation of human hepatocytes but with little effects on hepatocytes differentiation.
Khedr, M.E.M.S.
37c876ff-7226-4d51-bd20-dfe0bbca3d73
Abdelmotelb, Ahmed
cebf8ef6-9dbe-433d-b8ac-f9f8c0ee3f94
Bedwell, Tom A.
2db91b75-c2a9-468a-8d30-864bf5ca01e3
Abu-Hilal, Mohammed
2b7464e5-6a59-4bd8-bfe4-27a1918a5c5a
Khakoo, Salim
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273
Khedr, M.E.M.S.
37c876ff-7226-4d51-bd20-dfe0bbca3d73
Abdelmotelb, Ahmed
cebf8ef6-9dbe-433d-b8ac-f9f8c0ee3f94
Bedwell, Tom A.
2db91b75-c2a9-468a-8d30-864bf5ca01e3
Abu-Hilal, Mohammed
2b7464e5-6a59-4bd8-bfe4-27a1918a5c5a
Khakoo, Salim
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273

Khedr, M.E.M.S., Abdelmotelb, Ahmed, Bedwell, Tom A., Abu-Hilal, Mohammed and Khakoo, Salim (2016) Vasoactive intestinal peptide (VIP) induces proliferation of human hepatocytes. Faculty of Medicine Research Conference, University of Southampton, United Kingdom. 23 Jun 2016.

Record type: Conference or Workshop Item (Poster)

Abstract

Background: VIP is a gastrointestinal peptide hormone which regulates cell proliferation and differentiation in many cell types. In vitro, hepatocytes have limited viability and proliferative capacity. Although several growth factors such as epidermal growth factor (EGF) have been studied, role of VIP is still unclear. We have investigated the effect of VIP on proliferation of human hepatocytes.
METHODS: Human hepatocytes were isolated by two-steps collagenase protocol from liver specimens obtained from patients undergoing liver surgery. Cells were grown in Williams’ E media on collagen coated culture plate. Following 4 to 6 hours of cell plating, treatment with VIP or EGF was started and continued for 3 or 5 days. DNA replication was investigated by measuring Bromodeoxyuridine, BrdU incorporation using immunoflorescent staining. In parallel experiments; 1, 3 or 5 days total RNA was isolated and RT–PCR was performed. In the cell culture supernatant, urea and albumin concentrations were detected.
RESULTS: VIP was able to increase total number of hepatocytes and number of proliferating cells in a dose dependent manner markedly at day 3 of treatment. Treatment with VIP was associated with an increase in mRNA expression of ki-67 and H3 genes in a dose dependent process. Although mRNA expression of albumin gene was increased significantly with EGF, no marked alteration was found with VIP treatment. With increasing time, addition of VIP was associated with a decrease in albumin and urea secretion from liver cells.
CONCLUSIONS: VIP was able to induce proliferation of human hepatocytes but with little effects on hepatocytes differentiation.

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More information

Published date: 2016
Venue - Dates: Faculty of Medicine Research Conference, University of Southampton, United Kingdom, 2016-06-23 - 2016-06-23

Identifiers

Local EPrints ID: 432933
URI: http://eprints.soton.ac.uk/id/eprint/432933
PURE UUID: fb9803ba-71c7-4f26-84d7-57e6e64c24b1
ORCID for M.E.M.S. Khedr: ORCID iD orcid.org/0000-0001-9942-4409

Catalogue record

Date deposited: 01 Aug 2019 16:30
Last modified: 18 Feb 2021 17:20

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