A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings
A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings
Etiolated seedlings accumulate the chlorophyll biosynthesis intermediate protochlorophyllide (Pchlide) and measuring Pchlide can be important for characterizing photomorphogenic mutants that may be affected in chloroplast development. In this chapter we outline a simple and sensitive method for quantifying Pchlide in extracts of Arabidopsis seedlings using fluorescence spectroscopy. This method can be easily adapted to study chloroplast development in a wide range of plant species.
169-177
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Kacprzak, Sylwia M.
61c4bbb8-06e1-499d-89ee-290992e42a4e
2019
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Kacprzak, Sylwia M.
61c4bbb8-06e1-499d-89ee-290992e42a4e
Terry, Matthew J. and Kacprzak, Sylwia M.
(2019)
A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings.
In,
Hiltbrunner, A.
(ed.)
Methods in Molecular Biology: Phytochromes.
(Methods in Molecular Biology, 2026)
New York, NY.
Humana Press, .
(doi:10.1007/978-1-4939-9612-4_14).
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Abstract
Etiolated seedlings accumulate the chlorophyll biosynthesis intermediate protochlorophyllide (Pchlide) and measuring Pchlide can be important for characterizing photomorphogenic mutants that may be affected in chloroplast development. In this chapter we outline a simple and sensitive method for quantifying Pchlide in extracts of Arabidopsis seedlings using fluorescence spectroscopy. This method can be easily adapted to study chloroplast development in a wide range of plant species.
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Terry & Kacprzak - A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings pre-print
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Published date: 2019
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Local EPrints ID: 432948
URI: http://eprints.soton.ac.uk/id/eprint/432948
ISSN: 1064-3745
PURE UUID: bcd9d8ad-d1e7-486e-93ae-256369241a73
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Date deposited: 01 Aug 2019 16:30
Last modified: 17 Mar 2024 02:43
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Author:
Sylwia M. Kacprzak
Editor:
A. Hiltbrunner
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