The University of Southampton
University of Southampton Institutional Repository

A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings

A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings
A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings
Etiolated seedlings accumulate the chlorophyll biosynthesis intermediate protochlorophyllide (Pchlide) and measuring Pchlide can be important for characterizing photomorphogenic mutants that may be affected in chloroplast development. In this chapter we outline a simple and sensitive method for quantifying Pchlide in extracts of Arabidopsis seedlings using fluorescence spectroscopy. This method can be easily adapted to study chloroplast development in a wide range of plant species.
1064-3745
169-177
Humana Press
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Kacprzak, Sylwia M.
61c4bbb8-06e1-499d-89ee-290992e42a4e
Hiltbrunner, A.
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Kacprzak, Sylwia M.
61c4bbb8-06e1-499d-89ee-290992e42a4e
Hiltbrunner, A.

Terry, Matthew J. and Kacprzak, Sylwia M. (2019) A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings. In, Hiltbrunner, A. (ed.) Methods in Molecular Biology: Phytochromes. (Methods in Molecular Biology, 2026) New York, NY. Humana Press, pp. 169-177. (doi:10.1007/978-1-4939-9612-4_14).

Record type: Book Section

Abstract

Etiolated seedlings accumulate the chlorophyll biosynthesis intermediate protochlorophyllide (Pchlide) and measuring Pchlide can be important for characterizing photomorphogenic mutants that may be affected in chloroplast development. In this chapter we outline a simple and sensitive method for quantifying Pchlide in extracts of Arabidopsis seedlings using fluorescence spectroscopy. This method can be easily adapted to study chloroplast development in a wide range of plant species.

Text
Terry & Kacprzak - A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings pre-print
Download (555kB)

More information

Published date: 2019

Identifiers

Local EPrints ID: 432948
URI: http://eprints.soton.ac.uk/id/eprint/432948
ISSN: 1064-3745
PURE UUID: bcd9d8ad-d1e7-486e-93ae-256369241a73
ORCID for Matthew J. Terry: ORCID iD orcid.org/0000-0001-5002-2708

Catalogue record

Date deposited: 01 Aug 2019 16:30
Last modified: 17 Mar 2024 02:43

Export record

Altmetrics

Contributors

Author: Sylwia M. Kacprzak
Editor: A. Hiltbrunner

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×