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A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings

A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings
A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings

Etiolated seedlings accumulate the chlorophyll biosynthesis intermediate protochlorophyllide (Pchlide) and measuring Pchlide can be important for characterizing photomorphogenic mutants that may be affected in chloroplast development. In this chapter we outline a simple and sensitive method for quantifying Pchlide in extracts of Arabidopsis seedlings using fluorescence spectroscopy. This method can be easily adapted to study chloroplast development in a wide range of plant species.

1064-3745
169-177
Humana Press
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Kacprzak, Sylwia M.
61c4bbb8-06e1-499d-89ee-290992e42a4e
Hiltbrunner, A.
Terry, Matthew J.
a8c2cd6b-8d35-4053-8d77-3841c2427c3b
Kacprzak, Sylwia M.
61c4bbb8-06e1-499d-89ee-290992e42a4e
Hiltbrunner, A.

Terry, Matthew J. and Kacprzak, Sylwia M. (2019) A simple method for quantification of protochlorophyllide in etiolated Arabidopsis seedlings. In, Hiltbrunner, A. (ed.) Methods in Molecular Biology: Phytochromes. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-9612-4_14), 2026) New York, NY. Humana Press, pp. 169-177. (doi:10.1007/978-1-4939-9612-4_14).

Record type: Book Section

Abstract

Etiolated seedlings accumulate the chlorophyll biosynthesis intermediate protochlorophyllide (Pchlide) and measuring Pchlide can be important for characterizing photomorphogenic mutants that may be affected in chloroplast development. In this chapter we outline a simple and sensitive method for quantifying Pchlide in extracts of Arabidopsis seedlings using fluorescence spectroscopy. This method can be easily adapted to study chloroplast development in a wide range of plant species.

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Published date: 2019

Identifiers

Local EPrints ID: 432948
URI: http://eprints.soton.ac.uk/id/eprint/432948
ISSN: 1064-3745
PURE UUID: bcd9d8ad-d1e7-486e-93ae-256369241a73
ORCID for Matthew J. Terry: ORCID iD orcid.org/0000-0001-5002-2708

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Date deposited: 01 Aug 2019 16:30
Last modified: 12 Nov 2019 01:59

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