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An analytical differential resistance pulse system relying on a time shift signal analysis – applications in Coulter counting

An analytical differential resistance pulse system relying on a time shift signal analysis – applications in Coulter counting
An analytical differential resistance pulse system relying on a time shift signal analysis – applications in Coulter counting
Improving the sensitivity and ultimately the range of particle sizes that can be detected with a single pore extends the versatility of the Coulter counting technique. Here, to enable a pore to have greater sensitivity, we have developed and tested a novel differential resistive pulse sensing (DiS) system for sizing particles. To do this, the response was generated through a time shift approach utilizing a “self-servoing regime” to enable the final signal to operate with a zero background in the absence of particle translocation. The detection and characterization of a series of polystyrene particles, forced to translocate through a cylindrical glass microchannel (GMC) by a suitable static pressure difference using this approach, is demonstrated. An analytical response, which scales with the size of the particles employed, was verified. Parasitic capacitive effects are discussed; however, translocations on the millisecond time scale can be detected with high sensitivity and accuracy using the approach described.
translation, Coulter, differential, microchannel, sensitivity
2379-3694
2190-2195
Birkin, Peter R.
ba466560-f27c-418d-89fc-67ea4f81d0a7
Linfield, Steven
0c96c9cf-24f8-4729-b635-15deb2d26e9c
Denuault, Guy
5c76e69f-e04e-4be5-83c5-e729887ffd4e
Jones, Ronald
e8ab1ea4-a401-4043-8732-adc0e1d24b5a
Youngs, Jack
bdc2f78f-ae97-4faf-8930-1970ca697867
Wain, Emily
3d4db12d-122a-4dcd-ae93-12d6af2ab269
Birkin, Peter R.
ba466560-f27c-418d-89fc-67ea4f81d0a7
Linfield, Steven
0c96c9cf-24f8-4729-b635-15deb2d26e9c
Denuault, Guy
5c76e69f-e04e-4be5-83c5-e729887ffd4e
Jones, Ronald
e8ab1ea4-a401-4043-8732-adc0e1d24b5a
Youngs, Jack
bdc2f78f-ae97-4faf-8930-1970ca697867
Wain, Emily
3d4db12d-122a-4dcd-ae93-12d6af2ab269

Birkin, Peter R., Linfield, Steven, Denuault, Guy, Jones, Ronald, Youngs, Jack and Wain, Emily (2019) An analytical differential resistance pulse system relying on a time shift signal analysis – applications in Coulter counting. ACS Sensors, 4 (8), 2190-2195. (doi:10.1021/acssensors.9b01087).

Record type: Article

Abstract

Improving the sensitivity and ultimately the range of particle sizes that can be detected with a single pore extends the versatility of the Coulter counting technique. Here, to enable a pore to have greater sensitivity, we have developed and tested a novel differential resistive pulse sensing (DiS) system for sizing particles. To do this, the response was generated through a time shift approach utilizing a “self-servoing regime” to enable the final signal to operate with a zero background in the absence of particle translocation. The detection and characterization of a series of polystyrene particles, forced to translocate through a cylindrical glass microchannel (GMC) by a suitable static pressure difference using this approach, is demonstrated. An analytical response, which scales with the size of the particles employed, was verified. Parasitic capacitive effects are discussed; however, translocations on the millisecond time scale can be detected with high sensitivity and accuracy using the approach described.

Text
Birkin et al. Differential sensing manuscript updated accepted but not proof corrected - Accepted Manuscript
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More information

Accepted/In Press date: 10 July 2019
e-pub ahead of print date: 10 July 2019
Published date: 23 August 2019
Keywords: translation, Coulter, differential, microchannel, sensitivity

Identifiers

Local EPrints ID: 433115
URI: http://eprints.soton.ac.uk/id/eprint/433115
ISSN: 2379-3694
PURE UUID: c5435a8e-cfd1-44a9-b072-097c21aff8a7
ORCID for Peter R. Birkin: ORCID iD orcid.org/0000-0002-6656-4074
ORCID for Guy Denuault: ORCID iD orcid.org/0000-0002-8630-9492

Catalogue record

Date deposited: 08 Aug 2019 16:30
Last modified: 07 Oct 2020 06:48

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Contributors

Author: Peter R. Birkin ORCID iD
Author: Steven Linfield
Author: Guy Denuault ORCID iD
Author: Ronald Jones
Author: Jack Youngs
Author: Emily Wain

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