Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography
Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography
Low inherent contrast in soft tissues has been limiting the use of X-ray absorption micro-computed tomography (micro-CT) to access high-resolution structural information of animal organs. The staining agents used in micro-CT to improve the contrast fail in providing high-quality images of whole organs of animals due to diffusion problems of the staining agent into the sample. We demonstrate a staining protocol that incorporates a biochemical conditioning step prior to exposure to the staining agent that succeeds in overcoming the diffusion problems, thus quickly providing high-quality micro-CT images of whole organs of mammals. Besides of yielding non-distorted three-dimensional information at the same spatial resolution accessible in histological sections, micro-CT images of whole organs stained by our method enable easy screening of slices along any direction of the volume thus demonstrating new possibilities of structural analysis in biomedical science.
de S. e Silva, Juliana Martins
15900462-57c8-4f3a-b65a-a0802607fa27
Zanette, Irene
39ee899c-0aae-4fac-aec6-826f848a8022
Noel, Peter B.
61fa1092-4ad9-44a7-a2de-1f8a076dd0da
Cardoso, Mateus B.
553dc9ff-0751-4977-91a0-9a94b5b43544
Kimm, Melanie A.
aa709d60-c815-40bc-bf3b-48404c92959f
Pfeiffer, Franz
90bfb684-c442-458c-8f10-64d0e89cbef1
1 November 2015
de S. e Silva, Juliana Martins
15900462-57c8-4f3a-b65a-a0802607fa27
Zanette, Irene
39ee899c-0aae-4fac-aec6-826f848a8022
Noel, Peter B.
61fa1092-4ad9-44a7-a2de-1f8a076dd0da
Cardoso, Mateus B.
553dc9ff-0751-4977-91a0-9a94b5b43544
Kimm, Melanie A.
aa709d60-c815-40bc-bf3b-48404c92959f
Pfeiffer, Franz
90bfb684-c442-458c-8f10-64d0e89cbef1
de S. e Silva, Juliana Martins, Zanette, Irene, Noel, Peter B., Cardoso, Mateus B., Kimm, Melanie A. and Pfeiffer, Franz
(2015)
Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography.
Scientific Reports, 5, [14088].
(doi:10.1038/srep14088).
Abstract
Low inherent contrast in soft tissues has been limiting the use of X-ray absorption micro-computed tomography (micro-CT) to access high-resolution structural information of animal organs. The staining agents used in micro-CT to improve the contrast fail in providing high-quality images of whole organs of animals due to diffusion problems of the staining agent into the sample. We demonstrate a staining protocol that incorporates a biochemical conditioning step prior to exposure to the staining agent that succeeds in overcoming the diffusion problems, thus quickly providing high-quality micro-CT images of whole organs of mammals. Besides of yielding non-distorted three-dimensional information at the same spatial resolution accessible in histological sections, micro-CT images of whole organs stained by our method enable easy screening of slices along any direction of the volume thus demonstrating new possibilities of structural analysis in biomedical science.
Text
Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography
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Accepted/In Press date: 18 August 2015
e-pub ahead of print date: 25 September 2015
Published date: 1 November 2015
Identifiers
Local EPrints ID: 433295
URI: http://eprints.soton.ac.uk/id/eprint/433295
ISSN: 2045-2322
PURE UUID: 4a7e93ba-fce0-4521-a3ea-f81d444b5dc4
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Date deposited: 13 Aug 2019 16:30
Last modified: 16 Mar 2024 03:23
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Contributors
Author:
Juliana Martins de S. e Silva
Author:
Irene Zanette
Author:
Peter B. Noel
Author:
Mateus B. Cardoso
Author:
Melanie A. Kimm
Author:
Franz Pfeiffer
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