Measuring microglial turnover in the adult brain
Measuring microglial turnover in the adult brain
Microglia are the main resident immunocompetent cells of the brain with key roles in brain development, homeostasis, and function. Recent reports have started to shed light on the homeostatic mechanisms regulating the composition and turnover of the microglial population under physiological conditions from development to ageing, but our knowledge of the dynamics of microglia is incomplete. Therefore, it appears relevant to provide a standardized approach to quantify the turnover of microglia, with direct application to create a greater understanding of the dynamics of this cell population, and how it may contribute to the pathogenesis and/or progression of neurological disorders. Here we describe a robust immunohistochemical method to analyze microglial proliferation in mouse brain, aiming at providing a shared and universal approach to analyze microglial dynamics across different laboratories.
207-215
Gomez-Nicola, Diego
0680aa66-9dee-47cf-a8d3-e39c988f85b5
Fryatt, Gemma L.
afd66d34-31f7-4231-9a7c-50bc283646bd
Askew, Katharine E.
ffc96fb4-f94c-4cb7-8479-e9f0b2dae0c7
2019
Gomez-Nicola, Diego
0680aa66-9dee-47cf-a8d3-e39c988f85b5
Fryatt, Gemma L.
afd66d34-31f7-4231-9a7c-50bc283646bd
Askew, Katharine E.
ffc96fb4-f94c-4cb7-8479-e9f0b2dae0c7
Gomez-Nicola, Diego, Fryatt, Gemma L. and Askew, Katharine E.
(2019)
Measuring microglial turnover in the adult brain.
In,
Garaschuk, O. and Verkhratsky, A.
(eds.)
Microglia.
(Methods in Molecular Biology, 2034)
New York, NY.
Humana, .
(doi:10.1007/978-1-4939-9658-2_15).
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Abstract
Microglia are the main resident immunocompetent cells of the brain with key roles in brain development, homeostasis, and function. Recent reports have started to shed light on the homeostatic mechanisms regulating the composition and turnover of the microglial population under physiological conditions from development to ageing, but our knowledge of the dynamics of microglia is incomplete. Therefore, it appears relevant to provide a standardized approach to quantify the turnover of microglia, with direct application to create a greater understanding of the dynamics of this cell population, and how it may contribute to the pathogenesis and/or progression of neurological disorders. Here we describe a robust immunohistochemical method to analyze microglial proliferation in mouse brain, aiming at providing a shared and universal approach to analyze microglial dynamics across different laboratories.
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e-pub ahead of print date: 8 August 2019
Published date: 2019
Identifiers
Local EPrints ID: 433429
URI: http://eprints.soton.ac.uk/id/eprint/433429
ISSN: 1064-3745
PURE UUID: 3632ad4f-d5ab-4264-9cf6-a24dab070b2f
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Date deposited: 22 Aug 2019 16:30
Last modified: 16 Mar 2024 04:04
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Contributors
Author:
Katharine E. Askew
Editor:
O. Garaschuk
Editor:
A. Verkhratsky
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