The University of Southampton
University of Southampton Institutional Repository

Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system

Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system
Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system
The Mla pathway is believed to be involved in maintaining the asymmetrical Gram-negative outer membrane via retrograde phospholipid transport. The pathway is composed of three components: the outer membrane MlaA–OmpC/F complex, a soluble periplasmic protein, MlaC, and the inner membrane ATPase, MlaFEDB complex. Here, we solve the crystal structure of MlaC in its phospholipid-free closed apo conformation, revealing a pivoting β-sheet mechanism that functions to open and close the phospholipid-binding pocket. Using the apo form of MlaC, we provide evidence that the inner-membrane MlaFEDB machinery exports phospholipids to MlaC in the periplasm. Furthermore, we confirm that the phospholipid export process occurs through the MlaD component of the MlaFEDB complex and that this process is independent of ATP. Our data provide evidence of an apparatus for lipid export away from the inner membrane and suggest that the Mla pathway may have a role in anterograde phospholipid transport.
biochemistry, biological techniques, enzyme mechanisms, Lipids, pathogens
2058-5276
Hughes, Gareth W.
59c4356a-f958-40ae-8e90-a82d89c47b77
Hall, Stephen C.L.
7914ab4d-9474-41de-a002-b420c9557313
Laxton, Claire S.
6770c631-fc1b-46ce-b59f-b173ab5b529d
Sridhar, Pooja
abee4fd2-0481-44ab-bac9-bbaf009d63b8
Mahadi, Amirul H.
ff225f09-8cbd-4546-8c5b-757d332a7a0e
Hatton, Caitlin
0e5113b5-cd09-4947-a417-befe5a6a056f
Piggot, Thomas
75829b71-d73b-43d1-b24f-3e70c2c4d0c8
Wotherspoon, Peter J.
2aab2ac9-3456-464a-a198-c9594b915e15
Leney, Aneika C.
2a9b64c3-0804-41f8-92ca-5bf2ab92972c
Ward, Douglas G.
1bc9ecb2-2695-4f8d-b19c-ff2556d389c5
Jamshad, Mohammed
7e860c1a-8b79-4f98-9f0b-1d552e15739b
Spana, Vaclav
c6b722ca-7bb9-4536-a66b-2b006a3b67af
Cadby, Ian T.
a6e10011-3061-456c-8c85-db50573ad97f
Harding, Christopher
ccc303c5-864a-4be2-ba57-b903d2208c7d
Isom, Georgia L.
c301ebcb-5d31-40c0-92e6-870ebaf9dfff
Bryant, Jack A.
56bcf917-ce31-483e-b1c6-d64b42038457
Parr, Rebecca J.
81ceaccf-9af7-4f20-a15b-448a63be4961
Yakub, Yasin
a2ba919e-db8c-4310-8122-64eeb5210b86
Jeeves, Mark
58280e91-211f-429a-8be7-a063fa569a43
Huber, Damon
e1432c73-7831-4897-973b-a3e8b9c864c1
Henderson, Ian R.
c7d47d93-2054-4adc-88e6-a056fa01a807
Clifton, Luke A.
025a4531-f08a-4d2c-87c5-24d17f8afda4
Lovering, Andrew L.
836e794a-429e-4af7-80d0-20d1631355d4
Knowles, Timothy J.
d706116f-b201-4180-94ad-81ed0e1b33ad
Hughes, Gareth W.
59c4356a-f958-40ae-8e90-a82d89c47b77
Hall, Stephen C.L.
7914ab4d-9474-41de-a002-b420c9557313
Laxton, Claire S.
6770c631-fc1b-46ce-b59f-b173ab5b529d
Sridhar, Pooja
abee4fd2-0481-44ab-bac9-bbaf009d63b8
Mahadi, Amirul H.
ff225f09-8cbd-4546-8c5b-757d332a7a0e
Hatton, Caitlin
0e5113b5-cd09-4947-a417-befe5a6a056f
Piggot, Thomas
75829b71-d73b-43d1-b24f-3e70c2c4d0c8
Wotherspoon, Peter J.
2aab2ac9-3456-464a-a198-c9594b915e15
Leney, Aneika C.
2a9b64c3-0804-41f8-92ca-5bf2ab92972c
Ward, Douglas G.
1bc9ecb2-2695-4f8d-b19c-ff2556d389c5
Jamshad, Mohammed
7e860c1a-8b79-4f98-9f0b-1d552e15739b
Spana, Vaclav
c6b722ca-7bb9-4536-a66b-2b006a3b67af
Cadby, Ian T.
a6e10011-3061-456c-8c85-db50573ad97f
Harding, Christopher
ccc303c5-864a-4be2-ba57-b903d2208c7d
Isom, Georgia L.
c301ebcb-5d31-40c0-92e6-870ebaf9dfff
Bryant, Jack A.
56bcf917-ce31-483e-b1c6-d64b42038457
Parr, Rebecca J.
81ceaccf-9af7-4f20-a15b-448a63be4961
Yakub, Yasin
a2ba919e-db8c-4310-8122-64eeb5210b86
Jeeves, Mark
58280e91-211f-429a-8be7-a063fa569a43
Huber, Damon
e1432c73-7831-4897-973b-a3e8b9c864c1
Henderson, Ian R.
c7d47d93-2054-4adc-88e6-a056fa01a807
Clifton, Luke A.
025a4531-f08a-4d2c-87c5-24d17f8afda4
Lovering, Andrew L.
836e794a-429e-4af7-80d0-20d1631355d4
Knowles, Timothy J.
d706116f-b201-4180-94ad-81ed0e1b33ad

Hughes, Gareth W., Hall, Stephen C.L., Laxton, Claire S., Sridhar, Pooja, Mahadi, Amirul H., Hatton, Caitlin, Piggot, Thomas, Wotherspoon, Peter J., Leney, Aneika C., Ward, Douglas G., Jamshad, Mohammed, Spana, Vaclav, Cadby, Ian T., Harding, Christopher, Isom, Georgia L., Bryant, Jack A., Parr, Rebecca J., Yakub, Yasin, Jeeves, Mark, Huber, Damon, Henderson, Ian R., Clifton, Luke A., Lovering, Andrew L. and Knowles, Timothy J. (2019) Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system. Nature Microbiology. (doi:10.1038/s41564-019-0481-y).

Record type: Article

Abstract

The Mla pathway is believed to be involved in maintaining the asymmetrical Gram-negative outer membrane via retrograde phospholipid transport. The pathway is composed of three components: the outer membrane MlaA–OmpC/F complex, a soluble periplasmic protein, MlaC, and the inner membrane ATPase, MlaFEDB complex. Here, we solve the crystal structure of MlaC in its phospholipid-free closed apo conformation, revealing a pivoting β-sheet mechanism that functions to open and close the phospholipid-binding pocket. Using the apo form of MlaC, we provide evidence that the inner-membrane MlaFEDB machinery exports phospholipids to MlaC in the periplasm. Furthermore, we confirm that the phospholipid export process occurs through the MlaD component of the MlaFEDB complex and that this process is independent of ATP. Our data provide evidence of an apparatus for lipid export away from the inner membrane and suggest that the Mla pathway may have a role in anterograde phospholipid transport.

Full text not available from this repository.

More information

Accepted/In Press date: 3 May 2019
e-pub ahead of print date: 24 June 2019
Keywords: biochemistry, biological techniques, enzyme mechanisms, Lipids, pathogens

Identifiers

Local EPrints ID: 433439
URI: http://eprints.soton.ac.uk/id/eprint/433439
ISSN: 2058-5276
PURE UUID: 52cab591-e439-4399-89da-e6167e1ff20b

Catalogue record

Date deposited: 22 Aug 2019 16:30
Last modified: 21 Oct 2021 17:18

Export record

Altmetrics

Contributors

Author: Gareth W. Hughes
Author: Stephen C.L. Hall
Author: Claire S. Laxton
Author: Pooja Sridhar
Author: Amirul H. Mahadi
Author: Caitlin Hatton
Author: Thomas Piggot
Author: Peter J. Wotherspoon
Author: Aneika C. Leney
Author: Douglas G. Ward
Author: Mohammed Jamshad
Author: Vaclav Spana
Author: Ian T. Cadby
Author: Christopher Harding
Author: Georgia L. Isom
Author: Jack A. Bryant
Author: Rebecca J. Parr
Author: Yasin Yakub
Author: Mark Jeeves
Author: Damon Huber
Author: Ian R. Henderson
Author: Luke A. Clifton
Author: Andrew L. Lovering
Author: Timothy J. Knowles

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×