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Peptide nucleic acid probe, kit and method for detection and/or quantification of Salmonella SPP. and applications thereof

Peptide nucleic acid probe, kit and method for detection and/or quantification of Salmonella SPP. and applications thereof
Peptide nucleic acid probe, kit and method for detection and/or quantification of Salmonella SPP. and applications thereof
The present invention refers to the development of a Peptide Nucleic acid (PNA) probe for the detection of the Salmonella genus in different types of samples. PNA is a synthetic molecule analogue to DNA that, due to its physicochemical properties, allows a faster analysis with higher sensitivity than the DNA probes. These probes are combined with fluorescence in situ hybridization (FISH), a molecular biology technique that allows the direct visualization of the microorganism in the sample. The combination of these two technologies rendered the FISH procedure faster, simpler and more efficient. This probe can be applied to a great variety of samples such as blood, food, biopsies, feces, water and other clinical, environmental or agriculture and food industry samples. The present invention also includes the development of the kit of detection and respective procedure for the identification of Salmonella spp. using the above referred sample types.
WO2011121544
De Oliveira Azevedo, Nuno Filipe Ribeiro Pinto
aca410f4-7cd7-41d8-b4a7-07a37c7141c5
Da Costa Vieira, Maria Joao Lopes
b9f3c8a7-0d79-41a8-8483-eab06d81e19f
Almeida, Carina Manuela Fernandes
7f7ae90d-4105-4ff5-ba9f-772e908ac0bd
Keevil, Charles William
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

De Oliveira Azevedo, Nuno Filipe Ribeiro Pinto, Da Costa Vieira, Maria Joao Lopes, Almeida, Carina Manuela Fernandes and Keevil, Charles William (Inventors) (2011) Peptide nucleic acid probe, kit and method for detection and/or quantification of Salmonella SPP. and applications thereof. WO2011121544.

Record type: Patent

Abstract

The present invention refers to the development of a Peptide Nucleic acid (PNA) probe for the detection of the Salmonella genus in different types of samples. PNA is a synthetic molecule analogue to DNA that, due to its physicochemical properties, allows a faster analysis with higher sensitivity than the DNA probes. These probes are combined with fluorescence in situ hybridization (FISH), a molecular biology technique that allows the direct visualization of the microorganism in the sample. The combination of these two technologies rendered the FISH procedure faster, simpler and more efficient. This probe can be applied to a great variety of samples such as blood, food, biopsies, feces, water and other clinical, environmental or agriculture and food industry samples. The present invention also includes the development of the kit of detection and respective procedure for the identification of Salmonella spp. using the above referred sample types.

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Published date: 6 October 2011
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Identifiers

Local EPrints ID: 433866
URI: http://eprints.soton.ac.uk/id/eprint/433866
PURE UUID: 1fa0e0f8-b66e-41b5-8f6a-6349904b3e56
ORCID for Charles William Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 05 Sep 2019 16:30
Last modified: 18 Feb 2021 16:58

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Contributors

Inventor: Nuno Filipe Ribeiro Pinto De Oliveira Azevedo
Inventor: Maria Joao Lopes Da Costa Vieira
Inventor: Carina Manuela Fernandes Almeida
Inventor: Charles William Keevil ORCID iD

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