The University of Southampton
University of Southampton Institutional Repository

LC determination of indinavir in biological matrices with electrochemical detection

LC determination of indinavir in biological matrices with electrochemical detection
LC determination of indinavir in biological matrices with electrochemical detection

A high performance liquid chromatographic (HPLC) method with electrochemical detection for the quantification of Indinavir in cell culture is described. The sample pre-treatment involved a protein precipitation procedure using acetonitrile. Chromatography was carried out on a base-deactivated reversed-phase column with an isocratic mobile phase. The method was validated with regard to specificity, linearity, limits of detection and quantitation, precision and accuracy, recovery and ruggedness. The proposed HPLC assay was utilised to directly evaluate the capability of P-glycoprotein expressing multidrug resistant cells in mediating the transport and efflux of protease inhibitor (PI) Indinavir, a basic compound in AIDS care.

Anti-HIV Agents/analysis, Chromatography, High Pressure Liquid/methods, Electrochemistry, HIV Protease Inhibitors/analysis, Indinavir/analysis, Reproducibility of Results, Sensitivity and Specificity
0731-7085
307-314
Fizzano, M R
6deddd4d-e183-47b4-8ac6-8a62a51adf1d
Valvo, L
fc421f86-f15f-4720-8984-0c357c60391c
Dupuis, M L
29fc2be0-4439-478b-b262-f7dd2ecc642e
Mennella, V
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Cianfriglia, M
d7399b6d-82d2-4243-a6b3-ba0029f6526a
Fizzano, M R
6deddd4d-e183-47b4-8ac6-8a62a51adf1d
Valvo, L
fc421f86-f15f-4720-8984-0c357c60391c
Dupuis, M L
29fc2be0-4439-478b-b262-f7dd2ecc642e
Mennella, V
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Cianfriglia, M
d7399b6d-82d2-4243-a6b3-ba0029f6526a

Fizzano, M R, Valvo, L, Dupuis, M L, Mennella, V and Cianfriglia, M (2000) LC determination of indinavir in biological matrices with electrochemical detection. Journal of Pharmaceutical and Biomedical Analysis, 22 (2), 307-314. (doi:10.1016/s0731-7085(99)00288-5).

Record type: Article

Abstract

A high performance liquid chromatographic (HPLC) method with electrochemical detection for the quantification of Indinavir in cell culture is described. The sample pre-treatment involved a protein precipitation procedure using acetonitrile. Chromatography was carried out on a base-deactivated reversed-phase column with an isocratic mobile phase. The method was validated with regard to specificity, linearity, limits of detection and quantitation, precision and accuracy, recovery and ruggedness. The proposed HPLC assay was utilised to directly evaluate the capability of P-glycoprotein expressing multidrug resistant cells in mediating the transport and efflux of protease inhibitor (PI) Indinavir, a basic compound in AIDS care.

Full text not available from this repository.

More information

e-pub ahead of print date: 21 February 2000
Published date: March 2000
Keywords: Anti-HIV Agents/analysis, Chromatography, High Pressure Liquid/methods, Electrochemistry, HIV Protease Inhibitors/analysis, Indinavir/analysis, Reproducibility of Results, Sensitivity and Specificity

Identifiers

Local EPrints ID: 434032
URI: http://eprints.soton.ac.uk/id/eprint/434032
ISSN: 0731-7085
PURE UUID: 6fadf55c-d39f-4443-ba1c-c8e4bb971135
ORCID for V Mennella: ORCID iD orcid.org/0000-0002-4842-9012

Catalogue record

Date deposited: 11 Sep 2019 16:30
Last modified: 23 May 2020 00:46

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×