LC determination of indinavir in biological matrices with electrochemical detection
LC determination of indinavir in biological matrices with electrochemical detection
A high performance liquid chromatographic (HPLC) method with electrochemical detection for the quantification of Indinavir in cell culture is described. The sample pre-treatment involved a protein precipitation procedure using acetonitrile. Chromatography was carried out on a base-deactivated reversed-phase column with an isocratic mobile phase. The method was validated with regard to specificity, linearity, limits of detection and quantitation, precision and accuracy, recovery and ruggedness. The proposed HPLC assay was utilised to directly evaluate the capability of P-glycoprotein expressing multidrug resistant cells in mediating the transport and efflux of protease inhibitor (PI) Indinavir, a basic compound in AIDS care.
Anti-HIV Agents/analysis, Chromatography, High Pressure Liquid/methods, Electrochemistry, HIV Protease Inhibitors/analysis, Indinavir/analysis, Reproducibility of Results, Sensitivity and Specificity
307-314
Fizzano, M R
6deddd4d-e183-47b4-8ac6-8a62a51adf1d
Valvo, L
fc421f86-f15f-4720-8984-0c357c60391c
Dupuis, M L
29fc2be0-4439-478b-b262-f7dd2ecc642e
Mennella, V
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Cianfriglia, M
d7399b6d-82d2-4243-a6b3-ba0029f6526a
March 2000
Fizzano, M R
6deddd4d-e183-47b4-8ac6-8a62a51adf1d
Valvo, L
fc421f86-f15f-4720-8984-0c357c60391c
Dupuis, M L
29fc2be0-4439-478b-b262-f7dd2ecc642e
Mennella, V
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Cianfriglia, M
d7399b6d-82d2-4243-a6b3-ba0029f6526a
Fizzano, M R, Valvo, L, Dupuis, M L, Mennella, V and Cianfriglia, M
(2000)
LC determination of indinavir in biological matrices with electrochemical detection.
Journal of Pharmaceutical and Biomedical Analysis, 22 (2), .
(doi:10.1016/s0731-7085(99)00288-5).
Abstract
A high performance liquid chromatographic (HPLC) method with electrochemical detection for the quantification of Indinavir in cell culture is described. The sample pre-treatment involved a protein precipitation procedure using acetonitrile. Chromatography was carried out on a base-deactivated reversed-phase column with an isocratic mobile phase. The method was validated with regard to specificity, linearity, limits of detection and quantitation, precision and accuracy, recovery and ruggedness. The proposed HPLC assay was utilised to directly evaluate the capability of P-glycoprotein expressing multidrug resistant cells in mediating the transport and efflux of protease inhibitor (PI) Indinavir, a basic compound in AIDS care.
This record has no associated files available for download.
More information
e-pub ahead of print date: 21 February 2000
Published date: March 2000
Keywords:
Anti-HIV Agents/analysis, Chromatography, High Pressure Liquid/methods, Electrochemistry, HIV Protease Inhibitors/analysis, Indinavir/analysis, Reproducibility of Results, Sensitivity and Specificity
Identifiers
Local EPrints ID: 434032
URI: http://eprints.soton.ac.uk/id/eprint/434032
ISSN: 0731-7085
PURE UUID: 6fadf55c-d39f-4443-ba1c-c8e4bb971135
Catalogue record
Date deposited: 11 Sep 2019 16:30
Last modified: 16 Mar 2024 04:04
Export record
Altmetrics
Contributors
Author:
M R Fizzano
Author:
L Valvo
Author:
M L Dupuis
Author:
M Cianfriglia
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
