Uso di librerie fagiche per isolare in vitro anticorpi monoclonali ricombinanti di predeterminata specificità

Flego, Michela, Ascione, Alessandro, Pini, Alessandro, Mennella, Vito, Dupuis, Maria Luisa, Benagiano, Giuseppe and Cianfriglia, Maurizio (2002) Uso di librerie fagiche per isolare in vitro anticorpi monoclonali ricombinanti di predeterminata specificità. Annali dell'Istituto superiore di sanita, 38 (4), 401-410.

Abstract

The biotechnological generation of monoclonal antibodies of predetermined specificity has traditionally involved the production of hybridomas obtained by somatic cellular fusion of splenocytes from immunized animals with myeloma cell lines bearing selectable markers. Now, monoclonal antibodies could be genetically engineered thus bypassing all the natural systems for making antibodies. Filamentous bacteriophages provides a means to display and select large single chain fragments variable (scFv) repertoires created by cloning the natural rearranged V-immunoglobulin genes or introducing predetermined level of randomization into germline V-gene segments. In this article we demonstrated that by using a well characterized scFv phage synthetic library it is possible to generate in vitro recombinant human monoclonal antibodies directed to a large array of antigens showing different molecular weights, conformations and origins.

This record has no associated files available for download.

Contributors

Author: Vito Mennella

Download statistics