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Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome

Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome
Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome

Centrosomes are conserved organelles that are essential for accurate cell division and cilium formation. A centrosome consists of a pair of centrioles surrounded by a protein network of pericentriolar material (PCM) that is essential for the centrosome's function. In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole. When Sas-4 is absent, nascent procentrioles are unstable and lack PCM, and functional centrosomes are not generated. When Sas-4 is mutated, so that it cannot form S-CAP complexes, centrosomes are present but with dramatically reduced levels of PCM. Finally, purified S-CAP complexes or recombinant Sas-4 can bind centrosomes stripped of PCM, whereas recombinant CNN or Asl cannot. In summary, PCM assembly begins in the cytosol where Sas-4 provides a scaffold for pre-assembled cytoplasmic complexes before tethering of the complexes in a centrosome.

Animals, Animals, Genetically Modified, Blotting, Western, Centrosome/chemistry, Chromatography, Liquid, Cytoplasm/metabolism, Drosophila, Drosophila Proteins/genetics, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Fluorescent Antibody Technique, Macromolecular Substances/chemistry, Microscopy, Immunoelectron, Models, Molecular, Tandem Mass Spectrometry, Tubulin/metabolism
2041-1723
1-11
Gopalakrishnan, Jayachandran
e38d0fd6-b754-4e7d-9f1c-dbc0a173bb1a
Mennella, Vito
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Blachon, Stephanie
6393ab26-f8e6-4e5d-86de-daae599e56f2
Zhai, Bo
26277213-fb37-46d2-ae2e-b1e3d28d052f
Smith, Andrew H
f719dbf6-612c-4ecb-9ec8-ae0ac74928eb
Megraw, Timothy L
4238a01d-1391-4788-9628-c6d6cbd54477
Nicastro, Daniela
a36fde81-e08c-40f7-9a4e-b2dc86126bda
Gygi, Steven P
533b5ade-7f3c-4a9d-a324-33b4fdaa3908
Agard, David A
c0733eec-3b98-4a05-9cac-4a965769f5b1
Avidor-Reiss, Tomer
ad760347-f82b-46b6-8ef6-bd1f407acfd5
Gopalakrishnan, Jayachandran
e38d0fd6-b754-4e7d-9f1c-dbc0a173bb1a
Mennella, Vito
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Blachon, Stephanie
6393ab26-f8e6-4e5d-86de-daae599e56f2
Zhai, Bo
26277213-fb37-46d2-ae2e-b1e3d28d052f
Smith, Andrew H
f719dbf6-612c-4ecb-9ec8-ae0ac74928eb
Megraw, Timothy L
4238a01d-1391-4788-9628-c6d6cbd54477
Nicastro, Daniela
a36fde81-e08c-40f7-9a4e-b2dc86126bda
Gygi, Steven P
533b5ade-7f3c-4a9d-a324-33b4fdaa3908
Agard, David A
c0733eec-3b98-4a05-9cac-4a965769f5b1
Avidor-Reiss, Tomer
ad760347-f82b-46b6-8ef6-bd1f407acfd5

Gopalakrishnan, Jayachandran, Mennella, Vito, Blachon, Stephanie, Zhai, Bo, Smith, Andrew H, Megraw, Timothy L, Nicastro, Daniela, Gygi, Steven P, Agard, David A and Avidor-Reiss, Tomer (2011) Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nature Communications, 2, 1-11. (doi:10.1038/ncomms1367).

Record type: Article

Abstract

Centrosomes are conserved organelles that are essential for accurate cell division and cilium formation. A centrosome consists of a pair of centrioles surrounded by a protein network of pericentriolar material (PCM) that is essential for the centrosome's function. In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole. When Sas-4 is absent, nascent procentrioles are unstable and lack PCM, and functional centrosomes are not generated. When Sas-4 is mutated, so that it cannot form S-CAP complexes, centrosomes are present but with dramatically reduced levels of PCM. Finally, purified S-CAP complexes or recombinant Sas-4 can bind centrosomes stripped of PCM, whereas recombinant CNN or Asl cannot. In summary, PCM assembly begins in the cytosol where Sas-4 provides a scaffold for pre-assembled cytoplasmic complexes before tethering of the complexes in a centrosome.

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More information

Accepted/In Press date: 24 May 2011
Published date: 21 June 2011
Keywords: Animals, Animals, Genetically Modified, Blotting, Western, Centrosome/chemistry, Chromatography, Liquid, Cytoplasm/metabolism, Drosophila, Drosophila Proteins/genetics, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Fluorescent Antibody Technique, Macromolecular Substances/chemistry, Microscopy, Immunoelectron, Models, Molecular, Tandem Mass Spectrometry, Tubulin/metabolism

Identifiers

Local EPrints ID: 434037
URI: http://eprints.soton.ac.uk/id/eprint/434037
ISSN: 2041-1723
PURE UUID: 3320d6fb-51aa-4852-944f-dbf4502ccc33
ORCID for Vito Mennella: ORCID iD orcid.org/0000-0002-4842-9012
ORCID for Andrew H Smith: ORCID iD orcid.org/0000-0001-7321-4331

Catalogue record

Date deposited: 11 Sep 2019 16:30
Last modified: 03 Dec 2019 01:20

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Contributors

Author: Jayachandran Gopalakrishnan
Author: Vito Mennella ORCID iD
Author: Stephanie Blachon
Author: Bo Zhai
Author: Andrew H Smith ORCID iD
Author: Timothy L Megraw
Author: Daniela Nicastro
Author: Steven P Gygi
Author: David A Agard
Author: Tomer Avidor-Reiss

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