Subdiffraction resolution microscopy methods for analyzing centrosomes organization
Subdiffraction resolution microscopy methods for analyzing centrosomes organization
In this chapter, we describe the current methods of examining the structure of centrosomes by fluorescence subdiffraction microscopy. By using recently developed microscopy techniques, centrosomal proteins can now be examined in cells with a resolution of only a few nanometers, a level of molecular detail beyond the reach of traditional cell biology methods as confocal and widefield microscopy. We emphasize imaging by three-dimensional structured illumination microscopy, stochastic optical reconstruction microscopy, and quantitative approaches to image data analysis.
Animals, Cell Line, Centrosome/physiology, Drosophila melanogaster, Fluorescent Antibody Technique, Indirect, Microscopy, Fluorescence/methods
129-152
Mennella, Vito
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Hanna, Rachel
ee6c7553-b9fe-4a00-9383-032ffb20a6d4
Kim, Moshe
53536b6b-a5ee-4d6a-a314-448e952cb814
Mennella, Vito
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Hanna, Rachel
ee6c7553-b9fe-4a00-9383-032ffb20a6d4
Kim, Moshe
53536b6b-a5ee-4d6a-a314-448e952cb814
Mennella, Vito, Hanna, Rachel and Kim, Moshe
(2015)
Subdiffraction resolution microscopy methods for analyzing centrosomes organization.
Methods in Cell Biology, 129, .
(doi:10.1016/bs.mcb.2015.03.009).
Abstract
In this chapter, we describe the current methods of examining the structure of centrosomes by fluorescence subdiffraction microscopy. By using recently developed microscopy techniques, centrosomal proteins can now be examined in cells with a resolution of only a few nanometers, a level of molecular detail beyond the reach of traditional cell biology methods as confocal and widefield microscopy. We emphasize imaging by three-dimensional structured illumination microscopy, stochastic optical reconstruction microscopy, and quantitative approaches to image data analysis.
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e-pub ahead of print date: 27 May 2015
Keywords:
Animals, Cell Line, Centrosome/physiology, Drosophila melanogaster, Fluorescent Antibody Technique, Indirect, Microscopy, Fluorescence/methods
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Local EPrints ID: 434096
URI: http://eprints.soton.ac.uk/id/eprint/434096
ISSN: 0091-679X
PURE UUID: da441289-39ae-4e25-a674-4278bf392ae8
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Date deposited: 12 Sep 2019 16:30
Last modified: 16 Mar 2024 04:04
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Author:
Rachel Hanna
Author:
Moshe Kim
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