Super-resolution microscopy: from single molecules to supramolecular assemblies
Super-resolution microscopy: from single molecules to supramolecular assemblies
Super-resolution microscopy (SRM) methods have allowed scientists to exceed the diffraction limit of light, enabling the discovery and investigation of cellular structures at the nanometer scale, from individual proteins to entire organelles. In this review we survey the application of SRM in elucidating the structure of macromolecules in the native cellular environment. We emphasize how SRM can generate molecular maps of protein complexes and extract quantitative information on the number, size, distribution, and spatial organization of macromolecules. We discuss both the novel information that can be generated through SRM as well as the experimental considerations to examine while conducting such studies. With the increasing popularity of SRM in the biological sciences, this review will serve as a tool to navigate the range of applications and harness the power of SRM to elucidate biological structures.
Animals, Humans, Macromolecular Substances/ultrastructure, Microscopy, Electron/methods, Molecular Imaging/methods, Nanotechnology/methods, Organelles/ultrastructure
730-748
Sydor, Andrew M
93a9eb99-194a-4251-897d-6e7776b1e304
Czymmek, Kirk J
e01b7945-65bc-48b2-9c75-2a1446682bd7
Puchner, Elias M
7aa11501-f517-44ed-889b-309acd5403cf
Mennella, Vito
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
December 2015
Sydor, Andrew M
93a9eb99-194a-4251-897d-6e7776b1e304
Czymmek, Kirk J
e01b7945-65bc-48b2-9c75-2a1446682bd7
Puchner, Elias M
7aa11501-f517-44ed-889b-309acd5403cf
Mennella, Vito
43c60e29-c0a7-4ab8-8e5c-fcb59f70a28a
Sydor, Andrew M, Czymmek, Kirk J, Puchner, Elias M and Mennella, Vito
(2015)
Super-resolution microscopy: from single molecules to supramolecular assemblies.
Trends in Cell Biology, 25 (12), .
(doi:10.1016/j.tcb.2015.10.004).
Abstract
Super-resolution microscopy (SRM) methods have allowed scientists to exceed the diffraction limit of light, enabling the discovery and investigation of cellular structures at the nanometer scale, from individual proteins to entire organelles. In this review we survey the application of SRM in elucidating the structure of macromolecules in the native cellular environment. We emphasize how SRM can generate molecular maps of protein complexes and extract quantitative information on the number, size, distribution, and spatial organization of macromolecules. We discuss both the novel information that can be generated through SRM as well as the experimental considerations to examine while conducting such studies. With the increasing popularity of SRM in the biological sciences, this review will serve as a tool to navigate the range of applications and harness the power of SRM to elucidate biological structures.
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e-pub ahead of print date: 3 November 2015
Published date: December 2015
Keywords:
Animals, Humans, Macromolecular Substances/ultrastructure, Microscopy, Electron/methods, Molecular Imaging/methods, Nanotechnology/methods, Organelles/ultrastructure
Identifiers
Local EPrints ID: 434097
URI: http://eprints.soton.ac.uk/id/eprint/434097
ISSN: 0962-8924
PURE UUID: d862a6c9-b98c-45b4-8705-37a8058479ed
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Date deposited: 12 Sep 2019 16:30
Last modified: 16 Mar 2024 04:03
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Author:
Andrew M Sydor
Author:
Kirk J Czymmek
Author:
Elias M Puchner
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