The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Biocide susceptibilities and biofilm-forming capacities of Acinetobacter baumannii clinical isolates from Malaysia

Biocide susceptibilities and biofilm-forming capacities of Acinetobacter baumannii clinical isolates from Malaysia
Biocide susceptibilities and biofilm-forming capacities of Acinetobacter baumannii clinical isolates from Malaysia
Introduction. Acinetobacter baumannii is a Gram-negative nosocomial pathogen that has the capacity to develop resistance to all classes of antimicrobial compounds. However, very little is known regarding its susceptibility to biocides (antiseptics and disinfectants) and capacity to form biofilms, particularly for Malaysian isolates.Aim. To determine the susceptibility of A. baumannii isolates to commonly-used biocides, investigate their biofilm-forming capacities and the prevalence of biocide resistance and biofilm-associated genes.Methodology. The minimum inhibitory concentration (MIC) values of 100 A. baumannii hospital isolates from Terengganu, Malaysia, towards the biocides benzalkonium chloride (BZK), benzethonium chloride (BZT) and chlorhexidine digluconate (CLX), were determined by broth microdilution. The isolates were also examined for their ability to form biofilms in 96-well microplates. The prevalence of biocide resistance genes qacA, qacE and qacDE1 and the biofilm-associated genes bap and abaI were determined by polymerase chain reaction (PCR).Results. Majority of the A. baumannii isolates (43%) showed higher MIC values (> 50 µg/mL) for CLX than for BZK (5% for MIC > 50 µg/mL) and BZT (9% for MIC > 50 µg/mL). The qacDE1 gene was predominant (63%) followed by qacE (28%) whereas no isolate was found harbouring qacA. All isolates were positive for the bap and abaI genes although the biofilm-forming capacity varied among the isolates.Conclusion. The Terengganu A. baumannii isolates showed higher prevalence of qacDE1 compared to qacE although no correlation was found with the biocides’ MIC values. No correlation was also observed between the isolates’ biofilm-forming capacity and the MIC values for the biocides.
Acinetobacter baumannii, biocides, biofilm, chlorhexidine digluconate, benzalkolium chloride, benzethonium chloride
1972-2680
626-633
A'shimi, Muhammad Harith Nor
d80a43d8-0553-40cf-a2e2-99cffbabcd16
Alattraqchi, Ahmed Ghazi
dade11e6-1d45-4351-96a5-ed751091e200
Mohd Rani, Farahiyah
1a1e50e5-e44e-445e-83ef-c7039a713620
Rahman, Nor Iza A.
b4d18282-a17c-4cfd-9f6d-389642907c48
Ismail, Salwani
beb95eb3-3e79-4c48-a040-9994c7b5abce
Abdullah, Fatimah Haslina
ed358a29-5c46-4f41-a666-8e68f37a5fa9
Othman, Norlela
1fa2774c-ab30-4c12-8f43-4e92b9c9bacc
Cleary, David
f4079c6d-d54b-4108-b346-b0069035bec0
Clarke, Stuart
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Yeo, Chew Chieng
fdccc646-7c5d-4231-a9fa-ff01c39f4e5a
A'shimi, Muhammad Harith Nor
d80a43d8-0553-40cf-a2e2-99cffbabcd16
Alattraqchi, Ahmed Ghazi
dade11e6-1d45-4351-96a5-ed751091e200
Mohd Rani, Farahiyah
1a1e50e5-e44e-445e-83ef-c7039a713620
Rahman, Nor Iza A.
b4d18282-a17c-4cfd-9f6d-389642907c48
Ismail, Salwani
beb95eb3-3e79-4c48-a040-9994c7b5abce
Abdullah, Fatimah Haslina
ed358a29-5c46-4f41-a666-8e68f37a5fa9
Othman, Norlela
1fa2774c-ab30-4c12-8f43-4e92b9c9bacc
Cleary, David
f4079c6d-d54b-4108-b346-b0069035bec0
Clarke, Stuart
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Yeo, Chew Chieng
fdccc646-7c5d-4231-a9fa-ff01c39f4e5a

A'shimi, Muhammad Harith Nor, Alattraqchi, Ahmed Ghazi, Mohd Rani, Farahiyah, Rahman, Nor Iza A., Ismail, Salwani, Abdullah, Fatimah Haslina, Othman, Norlela, Cleary, David, Clarke, Stuart and Yeo, Chew Chieng (2019) Biocide susceptibilities and biofilm-forming capacities of Acinetobacter baumannii clinical isolates from Malaysia. The Journal of Infection in Developing Countries, 13 (7), 626-633. (doi:10.3855/jidc.11455).

Record type: Article

Abstract

Introduction. Acinetobacter baumannii is a Gram-negative nosocomial pathogen that has the capacity to develop resistance to all classes of antimicrobial compounds. However, very little is known regarding its susceptibility to biocides (antiseptics and disinfectants) and capacity to form biofilms, particularly for Malaysian isolates.Aim. To determine the susceptibility of A. baumannii isolates to commonly-used biocides, investigate their biofilm-forming capacities and the prevalence of biocide resistance and biofilm-associated genes.Methodology. The minimum inhibitory concentration (MIC) values of 100 A. baumannii hospital isolates from Terengganu, Malaysia, towards the biocides benzalkonium chloride (BZK), benzethonium chloride (BZT) and chlorhexidine digluconate (CLX), were determined by broth microdilution. The isolates were also examined for their ability to form biofilms in 96-well microplates. The prevalence of biocide resistance genes qacA, qacE and qacDE1 and the biofilm-associated genes bap and abaI were determined by polymerase chain reaction (PCR).Results. Majority of the A. baumannii isolates (43%) showed higher MIC values (> 50 µg/mL) for CLX than for BZK (5% for MIC > 50 µg/mL) and BZT (9% for MIC > 50 µg/mL). The qacDE1 gene was predominant (63%) followed by qacE (28%) whereas no isolate was found harbouring qacA. All isolates were positive for the bap and abaI genes although the biofilm-forming capacity varied among the isolates.Conclusion. The Terengganu A. baumannii isolates showed higher prevalence of qacDE1 compared to qacE although no correlation was found with the biocides’ MIC values. No correlation was also observed between the isolates’ biofilm-forming capacity and the MIC values for the biocides.

Text
Biocide susceptibilities and biofilm-forming capacities of Acinetobacter baumannii clinical isolates from Malaysia - Accepted Manuscript
Available under License Creative Commons Attribution.
Download (85kB)
Text
Biocide susceptibilities - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 26 June 2019
e-pub ahead of print date: 31 July 2019
Published date: 31 July 2019
Keywords: Acinetobacter baumannii, biocides, biofilm, chlorhexidine digluconate, benzalkolium chloride, benzethonium chloride

Identifiers

Local EPrints ID: 434104
URI: http://eprints.soton.ac.uk/id/eprint/434104
ISSN: 1972-2680
PURE UUID: 99934d70-769b-4d49-bf70-d79b28a16099
ORCID for David Cleary: ORCID iD orcid.org/0000-0003-4533-0700
ORCID for Stuart Clarke: ORCID iD orcid.org/0000-0002-7009-1548

Catalogue record

Date deposited: 12 Sep 2019 16:30
Last modified: 26 Nov 2021 03:02

Export record

Altmetrics

Contributors

Author: Muhammad Harith Nor A'shimi
Author: Ahmed Ghazi Alattraqchi
Author: Farahiyah Mohd Rani
Author: Nor Iza A. Rahman
Author: Salwani Ismail
Author: Fatimah Haslina Abdullah
Author: Norlela Othman
Author: David Cleary ORCID iD
Author: Stuart Clarke ORCID iD
Author: Chew Chieng Yeo

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×