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Tuning of salt separation efficiency by flow rate control in microfluidic dynamic dialysis

Tuning of salt separation efficiency by flow rate control in microfluidic dynamic dialysis
Tuning of salt separation efficiency by flow rate control in microfluidic dynamic dialysis
Microliter-scale separation processes are important for biomedical research and point-of-care diagnostics with small-volume clinical samples. Analytical assays such as mass spectrometry and field effect sensing necessitate sample desalting, but too low a salt concentration can disrupt protein structures and biomolecular interactions. In this work, we investigated whether salt extraction from a protein solution can be controlled by dynamic dialysis parameters. A microfluidic counter-flow dialyzer with a 5 kDa molecular weight cut-off cellulose membrane was fabricated by laser cutting and operated with a wide range of feed and dialysis flow rates. It was found that with the appropriate flow conditions, most notably the feed flow rate, retentate salt concentrations from 0.1 to 99% of the input NaCl concentration can be achieved. The experimental data were in good agreement with a theoretical diffusion-based mass transfer model. The salt dialysis performance was similar in the pres- ence of 50 mg/mL albumin, approximating blood plasma protein content, and did not deteriorate with overnight continuous dialysis, indicating minimal membrane fouling. The dialyzer construction method is compatible with all planar membranes, enabling implementation of tuneable dynamic dialysis for a wide range of on-line microfluidic biomolecular separations.
dynamic dialysis, desalting modulation, microfluidics, integrated membrane
1613-4982
1-11
Kalikavunkal, Prameen C.
dd0aa6ee-87db-4bbd-a142-0e3baf36cbc3
Green, Nicolas G.
d9b47269-c426-41fd-a41d-5f4579faa581
De Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Kalikavunkal, Prameen C.
dd0aa6ee-87db-4bbd-a142-0e3baf36cbc3
Green, Nicolas G.
d9b47269-c426-41fd-a41d-5f4579faa581
De Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba

Kalikavunkal, Prameen C., Green, Nicolas G. and De Planque, Maurits R.R. (2019) Tuning of salt separation efficiency by flow rate control in microfluidic dynamic dialysis. Microfluidics and Nanofluidics, 23 (111), 1-11. (doi:10.1007/s10404-019-2277-z).

Record type: Article

Abstract

Microliter-scale separation processes are important for biomedical research and point-of-care diagnostics with small-volume clinical samples. Analytical assays such as mass spectrometry and field effect sensing necessitate sample desalting, but too low a salt concentration can disrupt protein structures and biomolecular interactions. In this work, we investigated whether salt extraction from a protein solution can be controlled by dynamic dialysis parameters. A microfluidic counter-flow dialyzer with a 5 kDa molecular weight cut-off cellulose membrane was fabricated by laser cutting and operated with a wide range of feed and dialysis flow rates. It was found that with the appropriate flow conditions, most notably the feed flow rate, retentate salt concentrations from 0.1 to 99% of the input NaCl concentration can be achieved. The experimental data were in good agreement with a theoretical diffusion-based mass transfer model. The salt dialysis performance was similar in the pres- ence of 50 mg/mL albumin, approximating blood plasma protein content, and did not deteriorate with overnight continuous dialysis, indicating minimal membrane fouling. The dialyzer construction method is compatible with all planar membranes, enabling implementation of tuneable dynamic dialysis for a wide range of on-line microfluidic biomolecular separations.

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Accepted/In Press date: 24 August 2019
e-pub ahead of print date: 11 September 2019
Published date: October 2019
Keywords: dynamic dialysis, desalting modulation, microfluidics, integrated membrane

Identifiers

Local EPrints ID: 434123
URI: https://eprints.soton.ac.uk/id/eprint/434123
ISSN: 1613-4982
PURE UUID: a3885f47-2485-43f9-bc3f-d76d12ee99dd
ORCID for Nicolas G. Green: ORCID iD orcid.org/0000-0001-9230-4455
ORCID for Maurits R.R. De Planque: ORCID iD orcid.org/0000-0002-8787-0513

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Date deposited: 13 Sep 2019 16:30
Last modified: 03 Dec 2019 01:48

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