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Effects of sphagnum leachate on competitive sphagnum microbiome depend on species and time

Effects of sphagnum leachate on competitive sphagnum microbiome depend on species and time
Effects of sphagnum leachate on competitive sphagnum microbiome depend on species and time
Plant specialized metabolites play an important role in soil carbon (C) and nutrient fluxes. Through anti-microbial effects, they can modulate microbial assemblages and associated microbial-driven processes, such as nutrient cycling, so to positively or negatively cascade on plant fitness. As such, plant specialized metabolites can be used as a tool to supplant competitors. These compounds are little studied in bryophytes. This is especially notable in peatlands where Sphagnum mosses can dominate the vegetation and show strong interspecific competition. Sphagnum mosses form carpets where diverse microbial communities live and play a crucial role in Sphagnum fitness by regulating C and nutrient cycling. Here, by means of a microcosm experiment, we assessed to what extent moss metabolites of two Sphagnum species (S. fallax and S. divinum) modulate the competitive Sphagnum microbiome, with particular focus on microbial respiration. Using a reciprocal leachate experiment, we found that interactions between Sphagnum leachates and microbiome are species-specific. We show that both Sphagnum leachates differed in compound richness and compound relative abundance, especially sphagnum acid derivates, and that they include microbial-related metabolites. The addition of S. divinum leachate on the S. fallax microbiome immediately reduced microbial respiration (−95%). Prolonged exposition of S. fallax microbiome to S. divinum leachate destabilized the food web structure due to a modulation of microbial abundance. In particular, leachate addition decreased the biomass of testate amoebae and rotifers but increased that of ciliates. These changes did not influence microbial CO2 respiration, suggesting that the structural plasticity of the food web leads to its functional resistance through the replacement of species that are functionally redundant. In contrast, S. fallax leachate neither affected S. divinum microbial respiration, nor microbial biomass. We, however, found that S. fallax leachate addition stabilized the food web structure associated to S. divinum by changing trophic interactions among species. The differences in allelopathic effects between both Sphagnum leachates might impact their competitiveness and affect species distribution at local scale. Our study further paves the way to better understand the role of moss and microbial specialized metabolites in peatland C dynamics.
allelopathy and allelochemicals, metabolomics, microbial networks, microbial respiration and biomass, peatland, plant competition, plant-exudates, soil food-web
1664-302X
Hamard, Samuel
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Robroek, Bjorn J. M.
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Allard, Pierre-Marie
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Signarbieux, Constant
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Zhou, Shuaizhen
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Saesong, Tongchai
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de Baaker, Flore
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Buttler, Alexandre
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Chiapusio, Genevieve
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Wolfender, Jean-Luc
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Bragazza, Luca
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Jassey, Vincent E. J.
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Hamard, Samuel
faeb3e51-0030-4717-a544-fb33c1e1b92e
Robroek, Bjorn J. M.
06dcb269-687c-41db-ab73-f61899617f92
Allard, Pierre-Marie
aae25ec0-d4ce-48f7-8977-325ac89d9987
Signarbieux, Constant
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Zhou, Shuaizhen
89cc0a5f-3da6-4c49-a48b-9acfa94feed0
Saesong, Tongchai
db8bbf54-9f22-4d20-af32-46642f600f50
de Baaker, Flore
42ba54f0-63dd-4c55-a6d1-e777335d76d9
Buttler, Alexandre
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Chiapusio, Genevieve
1a4fd70c-bc8d-4cbd-9c21-212b3be27d0c
Wolfender, Jean-Luc
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Bragazza, Luca
297b335a-0a17-4c30-afbb-dfc5267f6688
Jassey, Vincent E. J.
17b9b576-bb3b-4739-830e-744034e5e24a

Hamard, Samuel, Robroek, Bjorn J. M., Allard, Pierre-Marie, Signarbieux, Constant, Zhou, Shuaizhen, Saesong, Tongchai, de Baaker, Flore, Buttler, Alexandre, Chiapusio, Genevieve, Wolfender, Jean-Luc, Bragazza, Luca and Jassey, Vincent E. J. (2019) Effects of sphagnum leachate on competitive sphagnum microbiome depend on species and time. Frontiers in Microbiology, 10, [2042]. (doi:10.3389/fmicb.2019.02042).

Record type: Article

Abstract

Plant specialized metabolites play an important role in soil carbon (C) and nutrient fluxes. Through anti-microbial effects, they can modulate microbial assemblages and associated microbial-driven processes, such as nutrient cycling, so to positively or negatively cascade on plant fitness. As such, plant specialized metabolites can be used as a tool to supplant competitors. These compounds are little studied in bryophytes. This is especially notable in peatlands where Sphagnum mosses can dominate the vegetation and show strong interspecific competition. Sphagnum mosses form carpets where diverse microbial communities live and play a crucial role in Sphagnum fitness by regulating C and nutrient cycling. Here, by means of a microcosm experiment, we assessed to what extent moss metabolites of two Sphagnum species (S. fallax and S. divinum) modulate the competitive Sphagnum microbiome, with particular focus on microbial respiration. Using a reciprocal leachate experiment, we found that interactions between Sphagnum leachates and microbiome are species-specific. We show that both Sphagnum leachates differed in compound richness and compound relative abundance, especially sphagnum acid derivates, and that they include microbial-related metabolites. The addition of S. divinum leachate on the S. fallax microbiome immediately reduced microbial respiration (−95%). Prolonged exposition of S. fallax microbiome to S. divinum leachate destabilized the food web structure due to a modulation of microbial abundance. In particular, leachate addition decreased the biomass of testate amoebae and rotifers but increased that of ciliates. These changes did not influence microbial CO2 respiration, suggesting that the structural plasticity of the food web leads to its functional resistance through the replacement of species that are functionally redundant. In contrast, S. fallax leachate neither affected S. divinum microbial respiration, nor microbial biomass. We, however, found that S. fallax leachate addition stabilized the food web structure associated to S. divinum by changing trophic interactions among species. The differences in allelopathic effects between both Sphagnum leachates might impact their competitiveness and affect species distribution at local scale. Our study further paves the way to better understand the role of moss and microbial specialized metabolites in peatland C dynamics.

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Accepted/In Press date: 20 August 2019
Published date: 6 September 2019
Keywords: allelopathy and allelochemicals, metabolomics, microbial networks, microbial respiration and biomass, peatland, plant competition, plant-exudates, soil food-web

Identifiers

Local EPrints ID: 434552
URI: http://eprints.soton.ac.uk/id/eprint/434552
ISSN: 1664-302X
PURE UUID: b544f2ba-868d-4a3a-bdee-9e02a952c19e
ORCID for Bjorn J. M. Robroek: ORCID iD orcid.org/0000-0002-6714-0652

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Date deposited: 01 Oct 2019 16:30
Last modified: 26 Nov 2021 03:09

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Contributors

Author: Samuel Hamard
Author: Bjorn J. M. Robroek ORCID iD
Author: Pierre-Marie Allard
Author: Constant Signarbieux
Author: Shuaizhen Zhou
Author: Tongchai Saesong
Author: Flore de Baaker
Author: Alexandre Buttler
Author: Genevieve Chiapusio
Author: Jean-Luc Wolfender
Author: Luca Bragazza
Author: Vincent E. J. Jassey

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