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Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei

Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei
Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei

Shrimp aquaculture is severely affected by WSSV. Despite an increasing effort to understand host/virus interaction by characterizing changes in gene expression (GE) following WSSV infection, the majority of published studies have focussed on a single time-point, providing limited insight on the development of host-pathogen interaction over the infection cycle. Using RNA-seq, we contrasted GE in gills of Litopenaeus vannamei at 1.5, 18 and 56 hours-post-infection (hpi), between WSSV-challenged and control shrimps. Time course analysis revealed 5097 differentially expressed genes: 63 DEGs were viral genes and their expression in WSSV group either peaked at 18 hpi (and decreased at 56 hpi) or increased linearly up to 56 hpi, suggesting a different role played by these genes during the course of infection. The remaining DEGs showed that WSSV altered the expression of metabolic, immune, apoptotic and cytoskeletal genes and was able to inhibit NF-κB and JAK/STAT pathways. Interestingly, GE changes were not consistent through the course of infection but were dynamic with time, suggesting the complexity of host-pathogen interaction. These data offer novel insights into the cellular functions that are affected during the course of infection and ultimately provide a valuable resource towards our understanding of the host-pathogen dynamics and its variation with time.

2045-2322
Peruzza, Luca
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Shekhar, M. S.
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Kumar, K. Vinaya
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Swathi, A.
487276ba-dcbc-4bce-8850-b375c864e492
Karthic, K.
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Hauton, Chris
7706f6ba-4497-42b2-8c6d-00df81676331
Vijayan, K. K.
0b05a8b9-f6c8-4c37-8b34-6012fc746830
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Shekhar, M. S.
7cc1c236-cf3e-42d5-a131-792ddd0af0c1
Kumar, K. Vinaya
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Swathi, A.
487276ba-dcbc-4bce-8850-b375c864e492
Karthic, K.
32f22523-7047-4fcd-bfbc-9bc703ad92ab
Hauton, Chris
7706f6ba-4497-42b2-8c6d-00df81676331
Vijayan, K. K.
0b05a8b9-f6c8-4c37-8b34-6012fc746830

Peruzza, Luca, Shekhar, M. S., Kumar, K. Vinaya, Swathi, A., Karthic, K., Hauton, Chris and Vijayan, K. K. (2019) Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei. Scientific Reports, 9 (1), [13509]. (doi:10.1038/s41598-019-49836-0).

Record type: Article

Abstract

Shrimp aquaculture is severely affected by WSSV. Despite an increasing effort to understand host/virus interaction by characterizing changes in gene expression (GE) following WSSV infection, the majority of published studies have focussed on a single time-point, providing limited insight on the development of host-pathogen interaction over the infection cycle. Using RNA-seq, we contrasted GE in gills of Litopenaeus vannamei at 1.5, 18 and 56 hours-post-infection (hpi), between WSSV-challenged and control shrimps. Time course analysis revealed 5097 differentially expressed genes: 63 DEGs were viral genes and their expression in WSSV group either peaked at 18 hpi (and decreased at 56 hpi) or increased linearly up to 56 hpi, suggesting a different role played by these genes during the course of infection. The remaining DEGs showed that WSSV altered the expression of metabolic, immune, apoptotic and cytoskeletal genes and was able to inhibit NF-κB and JAK/STAT pathways. Interestingly, GE changes were not consistent through the course of infection but were dynamic with time, suggesting the complexity of host-pathogen interaction. These data offer novel insights into the cellular functions that are affected during the course of infection and ultimately provide a valuable resource towards our understanding of the host-pathogen dynamics and its variation with time.

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s41598-019-49836-0 - Version of Record
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More information

Accepted/In Press date: 30 August 2019
e-pub ahead of print date: 18 September 2019
Published date: 18 September 2019

Identifiers

Local EPrints ID: 434578
URI: http://eprints.soton.ac.uk/id/eprint/434578
ISSN: 2045-2322
PURE UUID: 1495face-52d9-47dd-a710-5b24cb728cce
ORCID for Chris Hauton: ORCID iD orcid.org/0000-0002-2313-4226

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Date deposited: 02 Oct 2019 16:30
Last modified: 26 Nov 2021 02:38

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Contributors

Author: Luca Peruzza
Author: M. S. Shekhar
Author: K. Vinaya Kumar
Author: A. Swathi
Author: K. Karthic
Author: Chris Hauton ORCID iD
Author: K. K. Vijayan

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