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Effect of a 6-week “Mediterranean” dietary intervention on in vitro human embryo development: the Preconception Dietary Supplements in Assisted Reproduction double-blinded randomized controlled trial

Effect of a 6-week “Mediterranean” dietary intervention on in vitro human embryo development: the Preconception Dietary Supplements in Assisted Reproduction double-blinded randomized controlled trial
Effect of a 6-week “Mediterranean” dietary intervention on in vitro human embryo development: the Preconception Dietary Supplements in Assisted Reproduction double-blinded randomized controlled trial

Objective: To study the impact of increased dietary intake of omega-3 fatty acids, vitamin D, and olive oil for 6 weeks before in vitro fertilization (IVF) or IVF–intracytoplasmic sperm injection (ICSI) on morphokinetic markers of early embryo development. Design: A double-blinded randomized controlled trial. Setting: Academic IVF unit. Patient(s): A total of 111 couples undergoing IVF or IVF-ICSI were recruited. Interventions(s): Fifty-five couples received the 6-week study intervention of a daily supplement drink enriched with omega-3 fatty acids and vitamin D plus additional olive oil and olive oil–based spread, and 56 couples received the control intervention. Main Outcome Measure(s): The primary end point for the study was the time taken for completion of the second cell cycle after fertilization (CC2). Secondary end points included time to complete the third and fourth cell cycles (CC3 and CC4), the synchrony of the second and third cell cycles (S2 and S3), and the day 3 and day 5 Known Implantation Data Scores (KIDScores). Result(s): There was no difference in CC2 between the two groups. However, CC4 was accelerated in the study group compared with the control group, and a significantly shortened S3 as well as an increase in KIDScore on day 3 were observed, indicating improved embryo quality in the study group. Conclusion(s): This study demonstrates that a short period of dietary supplementation alters the rate of embryo cleavage. Further research is required to investigate the mechanisms that regulate this effect, and whether the impact on embryo development translates into improved clinical outcomes. Clinical Trial Registration Number: ISRCTN50956936

Diet, IVF, embryo development, morphokinetic markers, omega-3
0015-0282
260-269
Kermack, Alexandra
b9294c93-c736-4505-abe4-78bee493f1c2
Lowen, Philippa
f2514089-dc9b-49ce-8d8b-c790cad9bf9a
Wellstead, Susan J.
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Fisk, Helena
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Montag, Markus
db53b97c-cb02-4061-b150-3fc58d8c5835
Cheong, Ying
4efbba2a-3036-4dce-82f1-8b4017952c83
Osmond, Clive
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Houghton, Franchesca D.
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Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Macklon, Nick
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e
Kermack, Alexandra
b9294c93-c736-4505-abe4-78bee493f1c2
Lowen, Philippa
f2514089-dc9b-49ce-8d8b-c790cad9bf9a
Wellstead, Susan J.
26c61d1d-5c6d-425c-b5d3-c5da400a938e
Fisk, Helena
2483d346-75dd-41b3-a481-10f8bb39cd9f
Montag, Markus
db53b97c-cb02-4061-b150-3fc58d8c5835
Cheong, Ying
4efbba2a-3036-4dce-82f1-8b4017952c83
Osmond, Clive
2677bf85-494f-4a78-adf8-580e1b8acb81
Houghton, Franchesca D.
53946041-127e-45a8-9edb-bf4b3c23005f
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Macklon, Nick
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e

Kermack, Alexandra, Lowen, Philippa, Wellstead, Susan J., Fisk, Helena, Montag, Markus, Cheong, Ying, Osmond, Clive, Houghton, Franchesca D., Calder, Philip and Macklon, Nick (2020) Effect of a 6-week “Mediterranean” dietary intervention on in vitro human embryo development: the Preconception Dietary Supplements in Assisted Reproduction double-blinded randomized controlled trial. Fertility and Sterility, 113 (2), 260-269. (doi:10.1016/j.fertnstert.2019.09.041).

Record type: Article

Abstract

Objective: To study the impact of increased dietary intake of omega-3 fatty acids, vitamin D, and olive oil for 6 weeks before in vitro fertilization (IVF) or IVF–intracytoplasmic sperm injection (ICSI) on morphokinetic markers of early embryo development. Design: A double-blinded randomized controlled trial. Setting: Academic IVF unit. Patient(s): A total of 111 couples undergoing IVF or IVF-ICSI were recruited. Interventions(s): Fifty-five couples received the 6-week study intervention of a daily supplement drink enriched with omega-3 fatty acids and vitamin D plus additional olive oil and olive oil–based spread, and 56 couples received the control intervention. Main Outcome Measure(s): The primary end point for the study was the time taken for completion of the second cell cycle after fertilization (CC2). Secondary end points included time to complete the third and fourth cell cycles (CC3 and CC4), the synchrony of the second and third cell cycles (S2 and S3), and the day 3 and day 5 Known Implantation Data Scores (KIDScores). Result(s): There was no difference in CC2 between the two groups. However, CC4 was accelerated in the study group compared with the control group, and a significantly shortened S3 as well as an increase in KIDScore on day 3 were observed, indicating improved embryo quality in the study group. Conclusion(s): This study demonstrates that a short period of dietary supplementation alters the rate of embryo cleavage. Further research is required to investigate the mechanisms that regulate this effect, and whether the impact on embryo development translates into improved clinical outcomes. Clinical Trial Registration Number: ISRCTN50956936

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Kermack et al. PREPARE trial manuscript - Accepted Manuscript
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More information

Accepted/In Press date: 26 September 2019
e-pub ahead of print date: 20 December 2019
Published date: February 2020
Keywords: Diet, IVF, embryo development, morphokinetic markers, omega-3

Identifiers

Local EPrints ID: 434749
URI: http://eprints.soton.ac.uk/id/eprint/434749
ISSN: 0015-0282
PURE UUID: bfde3743-ac53-478e-8181-eeae7320f019
ORCID for Alexandra Kermack: ORCID iD orcid.org/0000-0002-9176-9426
ORCID for Helena Fisk: ORCID iD orcid.org/0000-0002-9534-3246
ORCID for Ying Cheong: ORCID iD orcid.org/0000-0001-7687-4597
ORCID for Clive Osmond: ORCID iD orcid.org/0000-0002-9054-4655
ORCID for Franchesca D. Houghton: ORCID iD orcid.org/0000-0002-5167-1694
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 08 Oct 2019 16:30
Last modified: 26 Nov 2021 07:12

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Contributors

Author: Philippa Lowen
Author: Susan J. Wellstead
Author: Helena Fisk ORCID iD
Author: Markus Montag
Author: Ying Cheong ORCID iD
Author: Clive Osmond ORCID iD
Author: Philip Calder ORCID iD
Author: Nick Macklon

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