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Soft X-ray contact microscopy of botanical material using laser produced plasmas or synchrotron radiation

Soft X-ray contact microscopy of botanical material using laser produced plasmas or synchrotron radiation
Soft X-ray contact microscopy of botanical material using laser produced plasmas or synchrotron radiation
The use of soft x-ray contact microscopy (SXCM) allows biological specimens to be imaged with a resolution better than that possible with the light microscope (LM), but not as good as that obtained with the transmission electron microscope (TEM). Unlike TEM however, it is possible to image wet biological specimens that require no pretreatment before imaging. For TEM specimens need to be chemically fixed, dehydrated, embedded and sectioned before examination, any one of these preparative steps can, and does, introduce artefacts, thus the development of any technique which eliminates or reduces the possibility of artefacts will be of great advantage.
433-437
Springer
Stead, A.D.
6c6f0e9d-2452-43ca-8a92-2c677c5b4017
Ford, T.W.
32791d78-9f83-4f20-9751-d8b9513d6852
Eason, R.W.
e38684c3-d18c-41b9-a4aa-def67283b020
Michette, A.G.
02e07bc0-d224-449e-bd35-8dafef7554cc
Nyring, W.
4b8ff0bd-861e-43a2-830e-3985858dd48a
Roser, R.
19ab55a7-6198-4a9f-8617-4c2136c6fe22
Stead, A.D.
6c6f0e9d-2452-43ca-8a92-2c677c5b4017
Ford, T.W.
32791d78-9f83-4f20-9751-d8b9513d6852
Eason, R.W.
e38684c3-d18c-41b9-a4aa-def67283b020
Michette, A.G.
02e07bc0-d224-449e-bd35-8dafef7554cc
Nyring, W.
4b8ff0bd-861e-43a2-830e-3985858dd48a
Roser, R.
19ab55a7-6198-4a9f-8617-4c2136c6fe22

Stead, A.D., Ford, T.W., Eason, R.W., Michette, A.G., Nyring, W. and Roser, R. (1988) Soft X-ray contact microscopy of botanical material using laser produced plasmas or synchrotron radiation. In X-Ray Microscopy II. vol. 56, Springer. pp. 433-437 . (doi:10.1007/978-3-540-39246-0_78).

Record type: Conference or Workshop Item (Paper)

Abstract

The use of soft x-ray contact microscopy (SXCM) allows biological specimens to be imaged with a resolution better than that possible with the light microscope (LM), but not as good as that obtained with the transmission electron microscope (TEM). Unlike TEM however, it is possible to image wet biological specimens that require no pretreatment before imaging. For TEM specimens need to be chemically fixed, dehydrated, embedded and sectioned before examination, any one of these preparative steps can, and does, introduce artefacts, thus the development of any technique which eliminates or reduces the possibility of artefacts will be of great advantage.

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Published date: 1988

Identifiers

Local EPrints ID: 435105
URI: http://eprints.soton.ac.uk/id/eprint/435105
PURE UUID: b3fdd042-f9de-42c9-9581-d2889a167dd4
ORCID for R.W. Eason: ORCID iD orcid.org/0000-0001-9704-2204

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Date deposited: 23 Oct 2019 16:30
Last modified: 18 Feb 2021 16:36

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