Stability of the different arms of a DNA tetrahedron and its interaction with a minor groove ligand
Stability of the different arms of a DNA tetrahedron and its interaction with a minor groove ligand
DNA strands can be designed to assemble into stable three-dimensional structures, based on Watson-Crick base pairing rules. The simplest of these is the DNA tetrahedron that is composed of four oligonucleotides. We have re-designed the sequence of a DNA tetrahedron so that it contains a single (AATT) binding site for the minor groove binding ligand Hoechst 33258. We examined the stability of this structure by placing fluorescent groups within each of its edges and have shown that all the edges melt at the same temperature in the absence of the ligand. The minor groove ligand still binds to its recognition sequence within the tetrahedron and increases the melting temperature of the folded complex. This ligand-induced stabilisation is propagated into the adjacent helical arms and the tetrahedron melts as a single entity in a cooperative fashion.
DNA tetrahedron, Hoechst 33258, Minor groove ligand, Nanostructure
Carter, Michael L.J.
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Rusling, David A.
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Gurr, Steven
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Brown, Tom
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Fox, Keith R.
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January 2020
Carter, Michael L.J.
80400cab-7073-469b-bae9-f29e61e33cff
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Gurr, Steven
c15fc593-be16-4b6e-aa10-b1e4efbdc14e
Brown, Tom
2816825b-9f1a-40c7-b7f0-2c660b702cc1
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Carter, Michael L.J., Rusling, David A., Gurr, Steven, Brown, Tom and Fox, Keith R.
(2020)
Stability of the different arms of a DNA tetrahedron and its interaction with a minor groove ligand.
Biophysical Chemistry, 256, [106270].
(doi:10.1016/j.bpc.2019.106270).
Abstract
DNA strands can be designed to assemble into stable three-dimensional structures, based on Watson-Crick base pairing rules. The simplest of these is the DNA tetrahedron that is composed of four oligonucleotides. We have re-designed the sequence of a DNA tetrahedron so that it contains a single (AATT) binding site for the minor groove binding ligand Hoechst 33258. We examined the stability of this structure by placing fluorescent groups within each of its edges and have shown that all the edges melt at the same temperature in the absence of the ligand. The minor groove ligand still binds to its recognition sequence within the tetrahedron and increases the melting temperature of the folded complex. This ligand-induced stabilisation is propagated into the adjacent helical arms and the tetrahedron melts as a single entity in a cooperative fashion.
Text
tetrahedron revised
- Accepted Manuscript
More information
Accepted/In Press date: 2 October 2019
e-pub ahead of print date: 21 October 2019
Published date: January 2020
Keywords:
DNA tetrahedron, Hoechst 33258, Minor groove ligand, Nanostructure
Identifiers
Local EPrints ID: 435171
URI: http://eprints.soton.ac.uk/id/eprint/435171
ISSN: 0301-4622
PURE UUID: bb1acba3-8a4a-4fb4-b0bd-b80c17f1791e
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Date deposited: 25 Oct 2019 16:30
Last modified: 17 Mar 2024 02:34
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Contributors
Author:
Michael L.J. Carter
Author:
David A. Rusling
Author:
Tom Brown
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