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Maternal obesity during pregnancy alters daily activity and feeding cycles, and hypothalamic clock gene expression in adult male mouse offspring

Maternal obesity during pregnancy alters daily activity and feeding cycles, and hypothalamic clock gene expression in adult male mouse offspring
Maternal obesity during pregnancy alters daily activity and feeding cycles, and hypothalamic clock gene expression in adult male mouse offspring
An obesogenic diet adversely affects the endogenous mammalian circadian clock, altering daily activity and metabolism, and resulting in obesity. We investigated whether an obese pregnancy can alter the molecular clock in the offspring hypothalamus, resulting in changes to their activity and feeding rhythms. Female mice were fed a control (C, 7% kcal fat) or high fat diet (HF, 45% kcal fat) before mating and throughout pregnancy. Male offspring were fed the C or HF diet postweaning, resulting in four offspring groups: C/C, C/HF, HF/C, and HF/HF. Daily activity and food intake were monitored, and at 15 weeks of age were killed at six time-points over 24 h. The clock genes Clock, Bmal1, Per2, and Cry2 in the suprachiasmatic nucleus (SCN) and appetite genes Npy and Pomc in the arcuate nucleus (ARC) were measured. Daily activity and feeding cycles in the HF/C, C/HF, and HF/HF offspring were altered, with increased feeding bouts and activity during the day and increased food intake but reduced activity at night. Gene expression patterns and levels of Clock, Bmal1, Per2, and Cry2 in the SCN and Npy and Pomc in the ARC were altered in HF diet-exposed offspring. The altered expression of hypothalamic molecular clock components and appetite genes, together with changes in activity and feeding rhythms, could be contributing to offspring obesity.
high fat diet; maternal obesity; pregnancy; circadian clocks; appetite; suprachiasmatic nucleus; arcuate nucleus; mouse; activity
1422-0067
1-19
Cleal, Jane
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Bruce, Kimberley D.
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Shearer, Jasmine L.
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Thomas, Hugh
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Plume, Jack
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Gregory, Louise
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Shepard, James
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Spiers-Fitzgerald, Kerry
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Mani, Ravi
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Lewis, Rohan
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Lillycrop, Karen
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Hanson, Mark
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Byrne, Christopher
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Cagampang, Felino
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Cleal, Jane
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Bruce, Kimberley D.
2065fe20-0e9e-4f1a-8463-71b832776fa1
Shearer, Jasmine L.
c9599d59-4fc0-4fe7-ada2-2a8acd88dae5
Thomas, Hugh
7466ae4b-c7c8-4b1c-9e44-c4a591c7a72d
Plume, Jack
4fe11537-a246-4226-9c79-936db1e11b36
Gregory, Louise
61c62645-4e07-4e70-8524-5b8de5db5190
Shepard, James
b058b392-40c7-463a-98a2-8c892ddfe86a
Spiers-Fitzgerald, Kerry
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Mani, Ravi
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Lewis, Rohan
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Lillycrop, Karen
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Hanson, Mark
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Byrne, Christopher
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Cagampang, Felino
7cf57d52-4a65-4554-8306-ed65226bc50e

Cleal, Jane, Bruce, Kimberley D., Shearer, Jasmine L., Thomas, Hugh, Plume, Jack, Gregory, Louise, Shepard, James, Spiers-Fitzgerald, Kerry, Mani, Ravi, Lewis, Rohan, Lillycrop, Karen, Hanson, Mark, Byrne, Christopher and Cagampang, Felino (2019) Maternal obesity during pregnancy alters daily activity and feeding cycles, and hypothalamic clock gene expression in adult male mouse offspring. International Journal of Molecular Sciences, 20 (21), 1-19, [5408]. (doi:10.3390/ijms20215408).

Record type: Special issue

Abstract

An obesogenic diet adversely affects the endogenous mammalian circadian clock, altering daily activity and metabolism, and resulting in obesity. We investigated whether an obese pregnancy can alter the molecular clock in the offspring hypothalamus, resulting in changes to their activity and feeding rhythms. Female mice were fed a control (C, 7% kcal fat) or high fat diet (HF, 45% kcal fat) before mating and throughout pregnancy. Male offspring were fed the C or HF diet postweaning, resulting in four offspring groups: C/C, C/HF, HF/C, and HF/HF. Daily activity and food intake were monitored, and at 15 weeks of age were killed at six time-points over 24 h. The clock genes Clock, Bmal1, Per2, and Cry2 in the suprachiasmatic nucleus (SCN) and appetite genes Npy and Pomc in the arcuate nucleus (ARC) were measured. Daily activity and feeding cycles in the HF/C, C/HF, and HF/HF offspring were altered, with increased feeding bouts and activity during the day and increased food intake but reduced activity at night. Gene expression patterns and levels of Clock, Bmal1, Per2, and Cry2 in the SCN and Npy and Pomc in the ARC were altered in HF diet-exposed offspring. The altered expression of hypothalamic molecular clock components and appetite genes, together with changes in activity and feeding rhythms, could be contributing to offspring obesity.

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Accepted/In Press date: 28 October 2019
Published date: 30 October 2019
Keywords: high fat diet; maternal obesity; pregnancy; circadian clocks; appetite; suprachiasmatic nucleus; arcuate nucleus; mouse; activity

Identifiers

Local EPrints ID: 435601
URI: http://eprints.soton.ac.uk/id/eprint/435601
ISSN: 1422-0067
PURE UUID: e48002ac-6aa4-4937-b2c9-78a1b72c1a9e
ORCID for Jane Cleal: ORCID iD orcid.org/0000-0001-7978-4327
ORCID for Rohan Lewis: ORCID iD orcid.org/0000-0003-4044-9104
ORCID for Karen Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489
ORCID for Mark Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for Christopher Byrne: ORCID iD orcid.org/0000-0001-6322-7753
ORCID for Felino Cagampang: ORCID iD orcid.org/0000-0003-4404-9853

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Date deposited: 13 Nov 2019 17:30
Last modified: 15 Apr 2021 01:39

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Contributors

Author: Jane Cleal ORCID iD
Author: Kimberley D. Bruce
Author: Jasmine L. Shearer
Author: Hugh Thomas
Author: Jack Plume
Author: Louise Gregory
Author: James Shepard
Author: Kerry Spiers-Fitzgerald
Author: Ravi Mani
Author: Rohan Lewis ORCID iD
Author: Karen Lillycrop ORCID iD
Author: Mark Hanson ORCID iD

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