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Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates

Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates
Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates
The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.
Biofilm, reproducibility, guidelines, microplate, spectrophotometry, fluorometry
1479-0505
1-8
Allkja, J.
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Bjarnsholt, T.
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Coenye, T.
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Cos, P.
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Fallarero, A.
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Joe, J.
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Harrison, J.J.
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Oliver, A.
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Lopes, S.P.
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Pereira, M. O.
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Ramage, G.
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Shirtliff, M.E.
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Stoodley, Paul
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Webb, J.S.
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Zaat, S.A.J.
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Goeres, D.M.
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Azevedo, N.F.
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Allkja, J.
7089f368-5f5d-44fe-bb05-3ee3ee61f718
Bjarnsholt, T.
2064713a-3108-4997-ad17-c7cc775c3d31
Coenye, T.
2bc8218a-b635-45e2-ad49-e38510476d5d
Cos, P.
2b51c90d-5af8-4ca3-b98d-7752f8f892d4
Fallarero, A.
25996fff-8fcc-4c0d-b40a-84229f540e56
Joe, J.
3aca9514-0eb7-4262-b69d-c2ff4f368c44
Harrison, J.J.
fd786a3c-405d-4f10-83b2-384aae351d76
Oliver, A.
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Lopes, S.P.
839c7a67-4334-4119-892b-7011b02e22d4
Pereira, M. O.
c8d6fc98-bc58-4007-afa3-e4b032820a93
Ramage, G.
4ff6ce2a-433e-4617-ba6e-6114856e0942
Shirtliff, M.E.
a56f2867-1b95-4254-b990-dc45868164db
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Webb, J.S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Zaat, S.A.J.
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Goeres, D.M.
330c64ec-0071-4a8b-b304-3159c7087f4f
Azevedo, N.F.
b8f06100-6f5d-47cd-99e1-68fc8a1da46e

Allkja, J., Bjarnsholt, T., Coenye, T., Cos, P., Fallarero, A., Joe, J., Harrison, J.J., Oliver, A., Lopes, S.P., Pereira, M. O., Ramage, G., Shirtliff, M.E., Stoodley, Paul, Webb, J.S., Zaat, S.A.J., Goeres, D.M. and Azevedo, N.F. (2020) Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates. Biofilms, 2, 1-8, [100010]. (doi:10.1016/j.bioflm.2019.100010).

Record type: Article

Abstract

The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.

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Accepted/In Press date: 10 November 2019
e-pub ahead of print date: 19 November 2019
Published date: December 2020
Keywords: Biofilm, reproducibility, guidelines, microplate, spectrophotometry, fluorometry

Identifiers

Local EPrints ID: 435937
URI: http://eprints.soton.ac.uk/id/eprint/435937
ISSN: 1479-0505
PURE UUID: 1a7ade58-8ea7-4217-9af2-9daf8f336a6c
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X
ORCID for J.S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

Catalogue record

Date deposited: 25 Nov 2019 17:30
Last modified: 19 Jan 2022 02:40

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Contributors

Author: J. Allkja
Author: T. Bjarnsholt
Author: T. Coenye
Author: P. Cos
Author: A. Fallarero
Author: J. Joe
Author: J.J. Harrison
Author: A. Oliver
Author: S.P. Lopes
Author: M. O. Pereira
Author: G. Ramage
Author: M.E. Shirtliff
Author: Paul Stoodley ORCID iD
Author: J.S. Webb ORCID iD
Author: S.A.J. Zaat
Author: D.M. Goeres
Author: N.F. Azevedo

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