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Comparison of SC fibers for fs Ti:Sapphire based hyperspectral CARS microscopy

Comparison of SC fibers for fs Ti:Sapphire based hyperspectral CARS microscopy
Comparison of SC fibers for fs Ti:Sapphire based hyperspectral CARS microscopy

Hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy is a rapidly developing field enabling label-free, chemically selective bio-imaging based on Raman signatures [1]. A significant factor limiting its clinical application is the complexity of current laser sources. A solution immediately relevant to two-photon excited fluorescence imaging laboratories is coherently broadening a fs Ti :Sapphire laser seed in a fiber to provide the Raman wavelengths via spectral-focusing (SF) CARS. The NKT fiber with two Zero Dispersion Wavelengths (ZDWs) is one option but the spectrum exhibits low Power Spectral Density (PSD) because of the large (>800 nm) spectral broadening. Here we perform the first systematic comparison sweeping (i) input pump power, (ii) pump wavelength and (iii) fiber length comparing the coherent SC from a femtoWHITE-CARS (2 ZDWs) fiber, a fiber with one ZDW offset above the seed wavelength, and an all-normal dispersion (ANDi) fiber. Starting with the seed laser polarisation aligned to a principal fiber axis, we show the total experimentally measured spectral output and importantly the polarisation resolved spectral component on the orthogonal axis, which is a measure of the nonlinear power-dependent depolarisation[2]. This orthogonal component will degrade the efficiency of the CARS signal but still contributes to the bio-toxicity that limits the maximum power for imaging. Finally, we show representative SF-CARS microscopy images to showcase the power of this technique.

Institute of Electrical and Electronics Engineers Inc.
Herdzik, K. P.
c94de69f-61c5-4ba0-bed5-dd13c5d9ec0b
Bourdakos, K. N.
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Richardson, D. J.
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3
Horak, Peter
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Price, J. H.V.
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Mahajan, S.
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Herdzik, K. P.
c94de69f-61c5-4ba0-bed5-dd13c5d9ec0b
Bourdakos, K. N.
83f6fc3a-db12-476b-9a78-4aad8756f82f
Richardson, D. J.
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3
Horak, Peter
520489b5-ccc7-4d29-bb30-c1e36436ea03
Price, J. H.V.
fddcce17-291b-4d01-bd38-8fb0453abdc8
Mahajan, S.
3e8fb3d0-f384-4182-ac26-b3063056a3c6

Herdzik, K. P., Bourdakos, K. N., Richardson, D. J., Horak, Peter, Price, J. H.V. and Mahajan, S. (2019) Comparison of SC fibers for fs Ti:Sapphire based hyperspectral CARS microscopy. In 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019. Institute of Electrical and Electronics Engineers Inc.. (doi:10.1109/CLEOE-EQEC.2019.8873199).

Record type: Conference or Workshop Item (Paper)

Abstract

Hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy is a rapidly developing field enabling label-free, chemically selective bio-imaging based on Raman signatures [1]. A significant factor limiting its clinical application is the complexity of current laser sources. A solution immediately relevant to two-photon excited fluorescence imaging laboratories is coherently broadening a fs Ti :Sapphire laser seed in a fiber to provide the Raman wavelengths via spectral-focusing (SF) CARS. The NKT fiber with two Zero Dispersion Wavelengths (ZDWs) is one option but the spectrum exhibits low Power Spectral Density (PSD) because of the large (>800 nm) spectral broadening. Here we perform the first systematic comparison sweeping (i) input pump power, (ii) pump wavelength and (iii) fiber length comparing the coherent SC from a femtoWHITE-CARS (2 ZDWs) fiber, a fiber with one ZDW offset above the seed wavelength, and an all-normal dispersion (ANDi) fiber. Starting with the seed laser polarisation aligned to a principal fiber axis, we show the total experimentally measured spectral output and importantly the polarisation resolved spectral component on the orthogonal axis, which is a measure of the nonlinear power-dependent depolarisation[2]. This orthogonal component will degrade the efficiency of the CARS signal but still contributes to the bio-toxicity that limits the maximum power for imaging. Finally, we show representative SF-CARS microscopy images to showcase the power of this technique.

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More information

Published date: 1 June 2019
Venue - Dates: 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019, ICM – International Congress Centre, Munich, Germany, 2019-06-22 - 2019-06-26

Identifiers

Local EPrints ID: 436331
URI: http://eprints.soton.ac.uk/id/eprint/436331
PURE UUID: 1c711ad4-fbab-4a77-90d7-4fabac2d366b
ORCID for D. J. Richardson: ORCID iD orcid.org/0000-0002-7751-1058
ORCID for Peter Horak: ORCID iD orcid.org/0000-0002-8710-8764
ORCID for J. H.V. Price: ORCID iD orcid.org/0000-0003-0256-9172

Catalogue record

Date deposited: 06 Dec 2019 17:30
Last modified: 10 Nov 2021 03:06

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Contributors

Author: K. P. Herdzik
Author: K. N. Bourdakos
Author: Peter Horak ORCID iD
Author: J. H.V. Price ORCID iD
Author: S. Mahajan

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