READ ME File For 'Rhinovirus 2A is the key protease responsible for instigating the early block to gene expression in infected cells'

Dataset DOI: 10.5258/SOTON/D0858

ReadMe Author: Christopher John McCormick, University of Southampton [ORCID:0000-0002-6155-9161]

This dataset supports the publication:
AUTHORS: David Smart, Irene Filippi, Cornelia Blume, Benjamin Smalley, Donna Davies, Christopher J. McCormick
TITLE: Rhinovirus 2A is the key protease responsible for instigating the early block to gene expression in infected cells
JOURNAL: Journal of Cell Science



This dataset contains:

Data from luciferase gene expression experiments in cells expressing the 2A and 3C proteases from human rhinovirus. It also contains data from immunofluorescent microscopy analysis looking at 3C epxression levels in the nucleus.

The figures are as follows:

Fig. 1  Combined expression of GFP and epitope-tagged HRV A16 proteases from a series of tricistronic expression constructs. 
Fig. 3  Validating the use of a dynamic luciferase reporter expression cassette
Fig. 4  Transfecting different quantities of a vector expressing the active form of the HRV proteases impacts on the rate at which Nluc activity is inhibited
Fig. 5  Comparison of the different tagged active protease vectors to block gene expression
Fig. 6  Expressing non-tagged HRV proteases in cells and their impact on gene expression
Fig. 7  Assessing the individual contribution made by 2A and 3C on the early shutdown of gene expression
Fig. 8  Visualizing the location of 3C when expressed with and without an NLS tag
Fig. S4 Assessing any early impact that 2A might have on its own EMCV IRES-depedent expression
Fig. S5	Assessing the individual contribution made by epitope tagged 2A and 3C on the early shutdown of gene expression


Date of data collection: November 2017 to November 2019

Information about geographic location of data collection: University of Southampton, U.K.

Licence: CC-BY

Related projects: AAIR charity award "Investigating whether a species-dependent rhinovirus response in infected cells might be linked to respiratory disease outcome" Dec 2015-Sept 2016



Date that the file was created: July, 2019