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Data from: The consequences of daily cyclic hypoxia on a European grass shrimp: from short-term responses to long-term effects

Data from: The consequences of daily cyclic hypoxia on a European grass shrimp: from short-term responses to long-term effects
Data from: The consequences of daily cyclic hypoxia on a European grass shrimp: from short-term responses to long-term effects
1. Salt marshes are a key coastal environment for their important role as nursery habitats for marine and estuarine fish and crustaceans. Salt marshes are variable environments where species can experience daily cyclic hypoxic stress, characterized by profound variations in oxygen partial pressure (pO2) from supersaturated conditions (~42kPa) to extremely hypoxic conditions (~3kPa) in ~12-hours. 2. Here, under laboratory conditions, we assessed the physiological consequences of exposing the shrimp Palaemon varians, a species commonly found in the salt marshes of northern Europe, to the daily cyclic hypoxic regime currently experienced in its habitat in August (7.1±1.8 hours day-1 below 4.0kPa). In the laboratory adults were kept at water pO2 <4.5kPa for 7-hours each night and in normoxic conditions for the rest of the time. 3. We recorded an acceleration of P. varians’ moult cycle, which was 15% shorter in animals kept in cyclic hypoxia compared to animals in normoxia. Similarly, the pattern of expression of two cuticular proteins over an entire moult cycle indicated an effect of cyclic hypoxia on moult stage-related genes. After 16 days, morphological changes to the gills were detected, with shrimps in cyclic hypoxia having a 13.6% larger lamellar surface area (measured in µm2/mg animal) than normoxic animals, which could improve gas exchange capacity. Overall, phenotypic and morphological data indicate that faster moulting is triggered in response to cyclic hypoxia, with the benefit that gill modifications can be prompted more rapidly in order to meet oxygen requirements of the body. 4. On the first experimental day, in cyclic hypoxic exposed animals, we recorded a 50% decrease in feeding rates (during hypoxic conditions) in comparison to normoxic animals. Similarly, ammonium excretion was reduced by 66-75% during the 1st and 21st experimental day. Body size was reduced by ~4% after 28 days. Females that reproduced in cyclic hypoxic conditions reduced the amount of yolk in each egg by ~24%. Overall, results underline how, in a decapod shrimp living in a key coastal environment, many physiological parameters are impaired by a cyclic hypoxic regime that is currently found in its natural habitat.
gill morphology, salt marshes, Northern Europe, feeding, Palaemon varians, Cyclic hypoxia, reproduction, ammonium excretion, moult cycle, transcriptomics, shrimp, body size
Dryad Digital Repository
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Gerdol, Marco
06665026-fd50-4cb6-b310-ede42435ab4f
Oliphant, Andrew
a080aa80-9deb-4e70-aadb-7c0b02600735
Wilcockson, David
ba84a5a7-c06c-4d54-ba4d-1113c607e28e
Pallavicini, Alberto
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Hawkins, Lawrence
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Thatje, Sven
f1011fe3-1048-40c0-97c1-e93b796e6533
Hauton, Christopher
7706f6ba-4497-42b2-8c6d-00df81676331
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Gerdol, Marco
06665026-fd50-4cb6-b310-ede42435ab4f
Oliphant, Andrew
a080aa80-9deb-4e70-aadb-7c0b02600735
Wilcockson, David
ba84a5a7-c06c-4d54-ba4d-1113c607e28e
Pallavicini, Alberto
250ed385-c46f-4011-92d8-34cb0039a627
Hawkins, Lawrence
9c4d1845-82db-4305-acb5-31b218ac9c0e
Thatje, Sven
f1011fe3-1048-40c0-97c1-e93b796e6533
Hauton, Christopher
7706f6ba-4497-42b2-8c6d-00df81676331

Peruzza, Luca, Gerdol, Marco, Oliphant, Andrew, Wilcockson, David, Pallavicini, Alberto, Hawkins, Lawrence, Thatje, Sven and Hauton, Christopher (2018) Data from: The consequences of daily cyclic hypoxia on a European grass shrimp: from short-term responses to long-term effects. Dryad Digital Repository doi:10.5061/DRYAD.D12635V [Dataset]

Record type: Dataset

Abstract

1. Salt marshes are a key coastal environment for their important role as nursery habitats for marine and estuarine fish and crustaceans. Salt marshes are variable environments where species can experience daily cyclic hypoxic stress, characterized by profound variations in oxygen partial pressure (pO2) from supersaturated conditions (~42kPa) to extremely hypoxic conditions (~3kPa) in ~12-hours. 2. Here, under laboratory conditions, we assessed the physiological consequences of exposing the shrimp Palaemon varians, a species commonly found in the salt marshes of northern Europe, to the daily cyclic hypoxic regime currently experienced in its habitat in August (7.1±1.8 hours day-1 below 4.0kPa). In the laboratory adults were kept at water pO2 <4.5kPa for 7-hours each night and in normoxic conditions for the rest of the time. 3. We recorded an acceleration of P. varians’ moult cycle, which was 15% shorter in animals kept in cyclic hypoxia compared to animals in normoxia. Similarly, the pattern of expression of two cuticular proteins over an entire moult cycle indicated an effect of cyclic hypoxia on moult stage-related genes. After 16 days, morphological changes to the gills were detected, with shrimps in cyclic hypoxia having a 13.6% larger lamellar surface area (measured in µm2/mg animal) than normoxic animals, which could improve gas exchange capacity. Overall, phenotypic and morphological data indicate that faster moulting is triggered in response to cyclic hypoxia, with the benefit that gill modifications can be prompted more rapidly in order to meet oxygen requirements of the body. 4. On the first experimental day, in cyclic hypoxic exposed animals, we recorded a 50% decrease in feeding rates (during hypoxic conditions) in comparison to normoxic animals. Similarly, ammonium excretion was reduced by 66-75% during the 1st and 21st experimental day. Body size was reduced by ~4% after 28 days. Females that reproduced in cyclic hypoxic conditions reduced the amount of yolk in each egg by ~24%. Overall, results underline how, in a decapod shrimp living in a key coastal environment, many physiological parameters are impaired by a cyclic hypoxic regime that is currently found in its natural habitat.

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More information

Published date: 2018
Keywords: gill morphology, salt marshes, Northern Europe, feeding, Palaemon varians, Cyclic hypoxia, reproduction, ammonium excretion, moult cycle, transcriptomics, shrimp, body size

Identifiers

Local EPrints ID: 436504
URI: http://eprints.soton.ac.uk/id/eprint/436504
PURE UUID: c5cf8eb1-fbd3-49db-8fc5-0cc617282961
ORCID for Lawrence Hawkins: ORCID iD orcid.org/0000-0002-9236-2396
ORCID for Christopher Hauton: ORCID iD orcid.org/0000-0002-2313-4226

Catalogue record

Date deposited: 11 Dec 2019 17:31
Last modified: 12 Dec 2019 01:39

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Contributors

Creator: Luca Peruzza
Creator: Marco Gerdol
Creator: Andrew Oliphant
Creator: David Wilcockson
Creator: Alberto Pallavicini
Creator: Lawrence Hawkins ORCID iD
Creator: Sven Thatje

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