Exploring protein structures by DNP-enhanced methyl solid-state NMR spectroscopy

Abstract

Although the rapid development of sensitivity-enhanced solid-state NMR (ssNMR) spectroscopy based on dynamic nuclear polarization (DNP) has enabled a broad range of novel applications in material and life sciences, further methodological improvements are needed to unleash the full potential of DNP-ssNMR. Here, a new methyl-based toolkit for exploring protein structures is presented, which combines signal-enhancement by DNP with heteronuclear Overhauser effect (hetNOE), carbon–carbon-spin diffusion (SD) and strategically designed isotope-labeling schemes. It is demonstrated that within this framework, methyl groups can serve as dynamic sensors for probing local molecular packing within proteins. Furthermore, they can be used as “NMR torches” to selectively enlighten their molecular environment, e.g., to selectively enhance the polarization of nuclei within residues of ligand-binding pockets. Finally, the use of ¹³C–¹³C spin diffusion enables probing carbon–carbon distances within the subnanometer range, which bridges the gap between conventional ¹³C-ssNMR methods and EPR spectroscopy. The applicability of these methods is directly shown on a large membrane protein, the light-driven proton pump green proteorhodopsin (GPR), which offers new insight into the functional mechanism of the early step of its photocycle.

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Identifiers

ORCID for Alexander J. Leeder: orcid.org/0000-0001-9271-6038

ORCID for Lynda J. Brown: orcid.org/0000-0002-5678-0814

ORCID for Richard C.D. Brown: orcid.org/0000-0003-0156-7087

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