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Bacteroides phages recovered from human and animal faecal matrices in rural Kenya

Bacteroides phages recovered from human and animal faecal matrices in rural Kenya
Bacteroides phages recovered from human and animal faecal matrices in rural Kenya
This data describes the recovering and isolation processes of Bacteroides spp. strains from human and cattle faecal sources from rural areas in Siaya County (Kenya), and occurred between 7th and 28th of June 2018. The data also includes the detection of bacteriophages (infecting these Bacteroides spp. host strains) in conjunction with traditional faecal indicator organisms in water sources from Kisumu and Siaya County (Kenya) occurring between June 18th 2018 and June 13th 2019. Exact location (coordinates) of the sample points are also described in the data set. A microbiological technique using Bile Esculin Bacteroides (BBE) agar was used for the recovering and isolation processes of Bacteroides spp. strains. Standard ISO (7899-2, 9308-1, 10705-2 and 10705-4) techniques, such as membrane filtration and the double-agar-layer methods, were used for the detection of bacteriophages and traditional faecal indicator organisms. The purpose of data collection was to develop new markers that could identify cattle and/or human sources of faecal contamination, which could be used as part of a Microbial Source Tracking (MST) tool box. Technicians and researchers from the University of Brighton (UK), University of Southampton (UK), from the Victoria Institute for Research on Environment and Development (VIRED) (KE) and from the Kenya Medical Research Institute (KEMRI) (KE) were responsible for the collection and interpretation of data. This dataset is embargoed until 31st July 2020
NERC Environmental Information Data Centre
Gomes da Silva, Diogo
0a247507-43aa-4853-a41f-25d9324d9866
Ebdon, James
3658faca-9200-40a0-93fa-214e20f25a9b
Mwangi, Thumbi
74b28f8d-fafb-432f-8129-394944f2ed1a
Okotto-Okotto, Joseph
a8cb5abe-ee03-4c93-978b-b02a02350e26
Wright, Jim
94990ecf-f8dd-4649-84f2-b28bf272e464
Gomes da Silva, Diogo
0a247507-43aa-4853-a41f-25d9324d9866
Ebdon, James
3658faca-9200-40a0-93fa-214e20f25a9b
Mwangi, Thumbi
74b28f8d-fafb-432f-8129-394944f2ed1a
Okotto-Okotto, Joseph
a8cb5abe-ee03-4c93-978b-b02a02350e26
Wright, Jim
94990ecf-f8dd-4649-84f2-b28bf272e464

Gomes da Silva, Diogo, Ebdon, James, Mwangi, Thumbi, Okotto-Okotto, Joseph and Wright, Jim (2020) Bacteroides phages recovered from human and animal faecal matrices in rural Kenya. NERC Environmental Information Data Centre doi:10.5285/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c [Dataset]

Record type: Dataset

Abstract

This data describes the recovering and isolation processes of Bacteroides spp. strains from human and cattle faecal sources from rural areas in Siaya County (Kenya), and occurred between 7th and 28th of June 2018. The data also includes the detection of bacteriophages (infecting these Bacteroides spp. host strains) in conjunction with traditional faecal indicator organisms in water sources from Kisumu and Siaya County (Kenya) occurring between June 18th 2018 and June 13th 2019. Exact location (coordinates) of the sample points are also described in the data set. A microbiological technique using Bile Esculin Bacteroides (BBE) agar was used for the recovering and isolation processes of Bacteroides spp. strains. Standard ISO (7899-2, 9308-1, 10705-2 and 10705-4) techniques, such as membrane filtration and the double-agar-layer methods, were used for the detection of bacteriophages and traditional faecal indicator organisms. The purpose of data collection was to develop new markers that could identify cattle and/or human sources of faecal contamination, which could be used as part of a Microbial Source Tracking (MST) tool box. Technicians and researchers from the University of Brighton (UK), University of Southampton (UK), from the Victoria Institute for Research on Environment and Development (VIRED) (KE) and from the Kenya Medical Research Institute (KEMRI) (KE) were responsible for the collection and interpretation of data. This dataset is embargoed until 31st July 2020

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More information

Published date: 31 July 2020

Identifiers

Local EPrints ID: 436584
URI: http://eprints.soton.ac.uk/id/eprint/436584
PURE UUID: f2b2b236-2c0a-4604-a558-b8a83ea114e7
ORCID for Jim Wright: ORCID iD orcid.org/0000-0002-8842-2181

Catalogue record

Date deposited: 16 Dec 2019 20:56
Last modified: 07 Feb 2024 02:41

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Contributors

Creator: Diogo Gomes da Silva
Creator: James Ebdon
Creator: Thumbi Mwangi
Creator: Joseph Okotto-Okotto
Creator: Jim Wright ORCID iD

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