Single-cell gene expression data of mESC differentiation time-course toward the neuronal lineage.
Single-cell gene expression data of mESC differentiation time-course toward the neuronal lineage.
Single-cell gene expression data generated using 96x96 fluidigm dynamic arrays. Experimental details including steps to reproduce data are included in the associated manuscript. Briefly, mouse embryonic stem cells kept in 2i+LIF conditions for four passages differentiated towards the neuronal lineage in N2B27 medium as described in the literature (Ying et al. 2003 - DOI: 10.1038/nbt780). At 0h, 24h, 48h, 72h, 96h, 120h and 168h individual cells were sorted by FACS based on light-scatter properties and deposited into 96-well plates containing lysis buffer and RT reagents before processing for qPCR using the Biomark HD.
Gene Expression, Quantitative Polymerase Chain Reaction, Pluripotent Stem Cell Differentiation, Time Series
Stumpf, Patrick
dfdb286c-b321-46d3-8ba2-85b3b4a7f092
Stumpf, Patrick
dfdb286c-b321-46d3-8ba2-85b3b4a7f092
Stumpf, Patrick
(2017)
Single-cell gene expression data of mESC differentiation time-course toward the neuronal lineage.
Mendeley Data
doi:10.17632/g2md5gbhz7.1
[Dataset]
Abstract
Single-cell gene expression data generated using 96x96 fluidigm dynamic arrays. Experimental details including steps to reproduce data are included in the associated manuscript. Briefly, mouse embryonic stem cells kept in 2i+LIF conditions for four passages differentiated towards the neuronal lineage in N2B27 medium as described in the literature (Ying et al. 2003 - DOI: 10.1038/nbt780). At 0h, 24h, 48h, 72h, 96h, 120h and 168h individual cells were sorted by FACS based on light-scatter properties and deposited into 96-well plates containing lysis buffer and RT reagents before processing for qPCR using the Biomark HD.
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Published date: 27 September 2017
Keywords:
Gene Expression, Quantitative Polymerase Chain Reaction, Pluripotent Stem Cell Differentiation, Time Series
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Local EPrints ID: 436622
URI: http://eprints.soton.ac.uk/id/eprint/436622
PURE UUID: 0d58340f-36ef-4fa8-87b5-b44d7e432d41
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Date deposited: 18 Dec 2019 17:31
Last modified: 04 Jan 2024 04:45
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Creator:
Patrick Stumpf
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