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Epigenome wide comparison of DNA methylation profile between paired umbilical cord blood and neonatal blood on Guthrie cards

Epigenome wide comparison of DNA methylation profile between paired umbilical cord blood and neonatal blood on Guthrie cards
Epigenome wide comparison of DNA methylation profile between paired umbilical cord blood and neonatal blood on Guthrie cards

DNA methylation (DNAm) in blood (umbilical cord blood and capillary blood collected after birth on Guthrie cards) during the perinatal period is being increasingly studied with the aim of identifying epigenetic markers of in utero environmental exposures or later disease development. However, the comparability in DNAm between these two sources is unknown. To this end, DNAm from the cord blood and capillary blood of 34 subjects in the Isle of Wight 3rd Generation Birth Cohort (68 samples) were included to assess the comparability. Differences in average DNAm (overall agreement), correlations in DNAm, and intra-class correlation coefficients (ICC) in DNAm between the two sources, at each of the 430,742 CpG sites, were evaluated. The results showed that a high proportion (70.1%) of the CpGs DNAm agreed between cord blood and neonatal blood on Guthrie cards. A small portion of CpGs showed high correlation (correlation ≥0.5) or high ICC (ICC ≥0.5) in DNAm of the whole genome. This proportion increased dramatically in differentially methylated regions (DMRs) that are associated with exposure to maternal smoking, between the two sources.

1559-2294
1-8
Jiang, Yu
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Wei, Jinfeng
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Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Ewart, Susan
28667421-3cf7-43d7-b1c3-ca27564938f7
Rezwan, Faisal I.
203f8f38-1f5d-485b-ab11-c546b4276338
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Arshad, Hasan
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Karmaus, Wilfried
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Jiang, Yu
4fa0a27b-8c06-4988-832a-8f5fb3a3f1ec
Wei, Jinfeng
7fe4fbdd-45da-491a-9964-0caae50b0db3
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Ewart, Susan
28667421-3cf7-43d7-b1c3-ca27564938f7
Rezwan, Faisal I.
203f8f38-1f5d-485b-ab11-c546b4276338
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Arshad, Hasan
917e246d-2e60-472f-8d30-94b01ef28958
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853

Jiang, Yu, Wei, Jinfeng, Zhang, Hongmei, Ewart, Susan, Rezwan, Faisal I., Holloway, John W., Arshad, Hasan and Karmaus, Wilfried (2019) Epigenome wide comparison of DNA methylation profile between paired umbilical cord blood and neonatal blood on Guthrie cards. Epigenetics, 1-8. (doi:10.1080/15592294.2019.1699983).

Record type: Article

Abstract

DNA methylation (DNAm) in blood (umbilical cord blood and capillary blood collected after birth on Guthrie cards) during the perinatal period is being increasingly studied with the aim of identifying epigenetic markers of in utero environmental exposures or later disease development. However, the comparability in DNAm between these two sources is unknown. To this end, DNAm from the cord blood and capillary blood of 34 subjects in the Isle of Wight 3rd Generation Birth Cohort (68 samples) were included to assess the comparability. Differences in average DNAm (overall agreement), correlations in DNAm, and intra-class correlation coefficients (ICC) in DNAm between the two sources, at each of the 430,742 CpG sites, were evaluated. The results showed that a high proportion (70.1%) of the CpGs DNAm agreed between cord blood and neonatal blood on Guthrie cards. A small portion of CpGs showed high correlation (correlation ≥0.5) or high ICC (ICC ≥0.5) in DNAm of the whole genome. This proportion increased dramatically in differentially methylated regions (DMRs) that are associated with exposure to maternal smoking, between the two sources.

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AAM Jiang et al. 2019 - Accepted Manuscript
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More information

Accepted/In Press date: 22 November 2019
e-pub ahead of print date: 9 December 2019

Identifiers

Local EPrints ID: 436677
URI: http://eprints.soton.ac.uk/id/eprint/436677
ISSN: 1559-2294
PURE UUID: 8e4fc419-3b0a-4e17-9476-328b56b7238b
ORCID for Faisal I. Rezwan: ORCID iD orcid.org/0000-0001-9921-222X
ORCID for John W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 20 Dec 2019 17:54
Last modified: 26 Nov 2021 05:54

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Contributors

Author: Yu Jiang
Author: Jinfeng Wei
Author: Hongmei Zhang
Author: Susan Ewart
Author: Faisal I. Rezwan ORCID iD
Author: Hasan Arshad
Author: Wilfried Karmaus

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